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Decalcification by perfusion. A new method for rapid softening of temporal bones

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dc.contributor.authorNilsson, Magnuses
dc.contributor.authorHellström, Stenes
dc.contributor.authorAlbiin, Nils
dc.date.accessioned2011-02-01T08:45:52Z
dc.date.available2011-02-01T08:45:52Z
dc.date.issued1991
dc.description.abstractWe describe a new technique, decalcification by perfusion, for the softening of bony tissue. The blood circulatory system was perfused in 16 rats via a cannula through the left heart ventricle with a fixative followed by New DecalcR (an acidic demineralizer) for 30-240 minutes. Perfusion decalcification for 120 minutes softened al1 heads and middle ear specimens could be easily sampled and prepared for studies by both light and electron microscope. For comparison, a conventional immersion technique required 72 hours of decalcification to accomplish softening. The perfusion technique considerably reduced the time needed to decalcify the tissue and preserved the morphology better than did the immersion procedure.es
dc.formatapplication/pdfes
dc.format.extent6es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/18244
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectDecalcificationes
dc.subjectPerfusiones
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::611 - Anatomíaes
dc.titleDecalcification by perfusion. A new method for rapid softening of temporal boneses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol. 6, nº 3 (1991)