Publication:
Decalcification by perfusion. A new method for rapid softening of temporal bones

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Decalcification by perfusion. A new method for rapid softening of temporal bones.pdf(3.43 MB)
Date
1991
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Authors
Nilsson, Magnus ; Hellström, Sten ; Albiin, Nils
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Publisher
Murcia : F. Hernández
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DOI
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Description
Abstract
We describe a new technique, decalcification by perfusion, for the softening of bony tissue. The blood circulatory system was perfused in 16 rats via a cannula through the left heart ventricle with a fixative followed by New DecalcR (an acidic demineralizer) for 30-240 minutes. Perfusion decalcification for 120 minutes softened al1 heads and middle ear specimens could be easily sampled and prepared for studies by both light and electron microscope. For comparison, a conventional immersion technique required 72 hours of decalcification to accomplish softening. The perfusion technique considerably reduced the time needed to decalcify the tissue and preserved the morphology better than did the immersion procedure.
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Decalcification , Perfusion
Citation
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http://hdl.handle.net/10201/18244
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Histology and histopathology Vol. 6, nº 3 (1991)
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