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Analytical validation of an automated assay for the measurement of adenosine deaminase (ADA) and its isoenzymes in saliva and a pilot evaluation of their changes in patients with SARS-CoV-2 infection

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dc.contributor.authorFranco Martínez, Lorena
dc.contributor.authorTecles, Fernando
dc.contributor.authorTorres Cantero, Alberto
dc.contributor.authorBernal, Enrique
dc.contributor.authorSan Lázaro, Indra
dc.contributor.authorAlcaraz, María José
dc.contributor.authorVicente Romero, Rosario
dc.contributor.authorLamy, Elsa
dc.contributor.authorSánchez Resalt, Cristina
dc.contributor.authorRubio, Camila P.
dc.contributor.authorTvarijonaviciute, Asta
dc.contributor.authorMartínez Subiela, Silvia
dc.contributor.authorCerón, José J.
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2024-02-09T09:36:11Z
dc.date.available2024-02-09T09:36:11Z
dc.date.issued2021-04-28
dc.description©2021. This document is the published, version of a Published Work that appeared in final form in Clinical Chemistry and Laboratory Medicine (CCLM). To access the final edited and published work see https://doi.org/10.1515/cclm-2021-0324
dc.description.abstractObjectives: The aim of the present study was to validate a commercially available automated assay for the measurement of total adenosine deaminase (tADA) and its isoenzymes (ADA1 and ADA2) in saliva in a fast and accurate way, and evaluate the possible changes of these analytes in individuals with SARS-CoV-2 infection. Methods: The validation, in addition to the evaluation of precision and accuracy, included the analysis of the effects of the main procedures that are currently being used for SARS-CoV-2 inactivation in saliva and a pilot study to evaluate the possible changes in salivary tADA and isoenzymes in individuals infected with SARS-CoV-2. Results: The automated assay proved to be accurate and precise, with intra- and inter-assay coefficients of variation below 8.2%, linearity under dilution linear regression with R2 close to 1, and recovery percentage between 80 and 120% in all cases. This assay was affected when the sample is treated with heat or SDS for virus inactivation but tolerated Triton X-100 and NP-40. Individuals with SARS-CoV-2 infection (n=71) and who recovered from infection (n=11) had higher mean values of activity of tADA and its isoenzymes than healthy individuals (n=35). Conclusions: tADA and its isoenzymes ADA1 and ADA2 can be measured accurately and precisely in saliva samples in a rapid, economical, and reproducible way and can be analyzed after chemical inactivation with Triton X-100 and NP-40. Besides, the changes observed in tADA and isoenzymes in individuals with COVID-19 open the possibility of their potential use as non-invasive biomarkers in this disease.es
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dc.identifier.citationClinical Chemistry and Laboratory Medicine (CCLM) 59( 9) (2021): 1592–1599
dc.identifier.doihttps://doi.org/10.1515/cclm-2021-0324
dc.identifier.issnPrint: 1434-6621
dc.identifier.issnElectronic: 1437-4331
dc.identifier.urihttp://hdl.handle.net/10201/139072
dc.languageenges
dc.relationSeneca Foundation 19894/GERM/15 Portuguese Science Foundation CEECIND/04397/2017es
dc.relation.publisherversionhttps://www.degruyter.com/document/doi/10.1515/cclm-2021-0324/htmles
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAnalytical validationes
dc.subjectAssayes
dc.subjectBiomarkerses
dc.subjectCOVID-19es
dc.subjectSARS-CoV-2es
dc.titleAnalytical validation of an automated assay for the measurement of adenosine deaminase (ADA) and its isoenzymes in saliva and a pilot evaluation of their changes in patients with SARS-CoV-2 infectiones
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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