Publication: Analytical validation of an automated assay for the measurement of adenosine deaminase (ADA) and its isoenzymes in saliva and a pilot evaluation of their changes in patients with SARS-CoV-2 infection
Authors
Franco Martínez, Lorena ; Tecles, Fernando ; Torres Cantero, Alberto ; Bernal, Enrique ; San Lázaro, Indra ; Alcaraz, María José ; Vicente Romero, Rosario ; Lamy, Elsa ; Sánchez Resalt, Cristina ; Rubio, Camila P. ; Tvarijonaviciute, Asta ; Martínez Subiela, Silvia ; Cerón, José J.
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Publisher
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DOI
https://doi.org/10.1515/cclm-2021-0324
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info:eu-repo/semantics/article
Description
©2021. This document is the published, version of a Published Work that appeared in final form in Clinical Chemistry and Laboratory Medicine (CCLM). To access the final edited and published work see https://doi.org/10.1515/cclm-2021-0324
Abstract
Objectives:
The aim of the present study was to validate a commercially available automated assay for the measurement of total adenosine deaminase (tADA) and its isoenzymes (ADA1 and ADA2) in saliva in a fast and accurate way, and evaluate the possible changes of these analytes in individuals with SARS-CoV-2 infection.
Methods:
The validation, in addition to the evaluation of precision and accuracy, included the analysis of the effects of the main procedures that are currently being used for SARS-CoV-2 inactivation in saliva and a pilot study to evaluate the possible changes in salivary tADA and isoenzymes in individuals infected with SARS-CoV-2.
Results:
The automated assay proved to be accurate and precise, with intra- and inter-assay coefficients of variation below 8.2%, linearity under dilution linear regression with R2 close to 1, and recovery percentage between 80 and 120% in all cases. This assay was affected when the sample is treated with heat or SDS for virus inactivation but tolerated Triton X-100 and NP-40. Individuals with SARS-CoV-2 infection (n=71) and who recovered from infection (n=11) had higher mean values of activity of tADA and its isoenzymes than healthy individuals (n=35).
Conclusions:
tADA and its isoenzymes ADA1 and ADA2 can be measured accurately and precisely in saliva samples in a rapid, economical, and reproducible way and can be analyzed after chemical inactivation with Triton X-100 and NP-40. Besides, the changes observed in tADA and isoenzymes in individuals with COVID-19 open the possibility of their potential use as non-invasive biomarkers in this disease.
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Citation
Clinical Chemistry and Laboratory Medicine (CCLM) 59( 9) (2021): 1592–1599
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