Publication: The kinetics and distribution of different macrophage populations in the developing rat skin
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Date
2010
Authors
Juniantito, Vetnizah ; Izawa, Takeshi ; Yamamoto Emi ; Kuwamura, Mitsuru ; Yamate, Jyoji
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Macrophages play important roles in host
defense and homeostasis. In contrast to adulthood, far
less is known about macrophage populations in fetuses
and neonates. Macrophages were evaluated in the
developing rat skin at different anatomical sites (head,
anterior dorsal, posterior dorsal, and abdomen) of F344
rats obtained on gestational days 18 and 20, on neonatal
days 1-21, and at adult weeks 5-15. The numbers of
macrophages in the epidermis, dermis or perifollicular
areas that were positive for ED1 (exudative
macrophages with activated phagocytosis), ED2
(resident macrophages), and OX6 (antigen-presenting
cells) were evaluated. There were no differences in
macrophage numbers among the anatomical sites. In the
epidermis, only OX6 cells were seen, with gradually
increased numbers in neonates and adults. In the dermis,
many ED1 cells were already seen in fetuses, and the
number peaked on neonatal day 4, and remained at that
level until adulthood. By contrast, ED2 and OX6 cells
began to be seen after birth and their numbers continued
to increase until adulthood; ED2 cells were distributed
diffusely in the dermis, whereas ED1 and OX6 cells
were present exclusively in the upper dermis. In
perifollicular areas, ED1, ED2 and OX6 cells began to
be seen after birth, and their numbers gradually
increased until adulthood. Some macrophages in dermal
and perifollicular areas gave double-positive reactions to
ED1+ED2+, ED1+OX6+ or OX6+ED2+. Increased
mRNA levels of colony stimulating factor-1 and
monocyte chemoattractant protein-1 appeared to
correspond to the emergence of rat macrophages. Skin
macrophages were shown to be heterogeneous in
distribution and function; the information from this study should be very useful for future investigations of
experimentally induced rat skin lesions.
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