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PTPIP51, a positive modulator of the MAPK/Erk pathway, is upregulated in glioblastoma and interacts with 14-3-3ß and PTP1B in situ

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dc.contributor.authorPetri, M.K.
dc.contributor.authorStenzinger, A.
dc.contributor.authorKuchelmeister, K.
dc.contributor.authorNestler, U.
dc.contributor.authorParadowska, A.
dc.contributor.authorSteger, K.
dc.contributor.authorBrobeil, A.
dc.contributor.authorViard, M.
dc.contributor.authorWimmer, M.
dc.date.accessioned2016-05-03T16:34:40Z
dc.date.available2016-05-03T16:34:40Z
dc.date.issued2011
dc.description.abstractGlioblastoma multiforme (GBM) is the most common and most malignant primary brain tumour. Protein tyrosine phosphatase interacting protein 51 (PTPIP51) is an interaction partner of 14-3-3ß, which correlates with the grade of malignancy in gliomas. In this study PTPIP51 and its interacting partners 14-3-3ß, PTP1B, c-Src, Raf-1 as well as EGFR were investigated in human glioblastoma. Twenty glioblastoma samples were analyzed on transcriptional and translational level by immunohisto-chemistry, in situ hybridization and RT-PCR. To compare PTPIP51 expression in gliomas of different malignancies, quantitative RT-PCR for grade II astrocytoma and GBM samples was employed. Additionally, we analyzed the correlation between PTPIP51 and 14-3-3ß transcription, and checked for in situ interaction between PTPIP51 and 14-3-3ß and PTP1B, respectively. PTPIP51 and 14-3-3ß mRNA showed a tumour grade dependent upregulation in gliomas. Glioblastoma cells displayed a strong immunoreaction of PTPIP51, which co-localized with 14-3-3ß and PTP1B. The duolink proximity ligation assay corroborated a direct in situ interaction of PTPIP51 with both proteins, known to interact with PTPIP51 in vitro. The in vitro interacting partners Raf-1 and c-Src showed a partial co-localization. Besides, immune cells located in capillaries or infiltrating the tumour tissue and endothelial cells of pseudoglomerular vessels revealed a high PTPIP51 expression. The upregulation of PTPIP51 and its connection with the EGFR/MAPK pathway by 14-3-3ß via Raf-1 and by PTP1B via c-Src, argue for a functional role of PTPIP51 in the pathogenesis of human glioblastoma.es
dc.formatapplication/pdfes
dc.format.extent13es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/49529
dc.languageenges
dc.publisherF. Hernández y J.F. Madrid. Murcia: Universidad de Murcia, Departamento de Biología Celular e Histología.es
dc.relation.ispartofHistology and histopathology, Vol. 26, nº12 (2011)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectGlioblastomaes
dc.subjectPTPIP51es
dc.subject.other616 - Patología. Medicina clínica. Oncologíaes
dc.titlePTPIP51, a positive modulator of the MAPK/Erk pathway, is upregulated in glioblastoma and interacts with 14-3-3ß and PTP1B in situes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.26,nº12 (2011)