Publication: Differential expression of calretinin in the developing and regenerating zebrafish visual system
Authors
García-Crespo, D. ; Vecino, E.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Calretinin is a calcium-binding protein which
participates in a variety of functions including calcium
buffering and neuronal protection. It also serves as a
developmental marker of retinal ganglion cells (RGCs).
In order to study the role of calretinin in the
development and regeneration of RGCs, we have studied
its pattern of expression in the retina at different
developmental stages, as well as during optic nerve
regeneration by means of immunohistochemistry. During
development, calretinin is found for the first time in
RGCs when they connect with the optic tectum. Optic
nerves from adult zebrafish were crushed and after
different survival times, calretinin expression in the
retina, optic nerve tract and optic tectum was studied.
From the day of crushing to 10 days later, calretinin
expression was found to be downregulated within RGCs
and their axons, as was also observed during the early
developmental stages of RGCs, when they are not
committed to a definite cell phenotype. Moreover, 13
days after lesion, when the regenerating axons arrived at
the optic tectum, a recovery of calretinin
immunoreactivity within the RGCs was observed. These
results indicate that calretinin may play an important role
during optic nerve regeneration, Thus, the downregulation
of Calretinin during the growth of the RGC
axons towards the target during development as well as during their regeneration after injury, indicates that an
increase the availability of cytosolic calcium is integral
to axon outgrowth thus recapitulating the pattern
observed during development.
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