Publication: Gut glycoconjugates in Sparus aurata L. Pisces, Teleostei. A comparative histochemical study in larval and adult ages
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Date
1998
Authors
Domeneghini, C. ; Pannelli Straini, R. ; Veggetti, A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
This study examined the gut of the euryaline
fish Sparus aurata, from the pharynx to the rectum. The
specimens were collected from adult animals, both
sexes, and several larval and juvenile stages, from 4 to
135 days of age. Histochemical methods to distinguish
neutral and acidic glycoconjugates, as well as specific
techniques to identify acidic glycoconjugates which
contained 0-acylated sialic acids were used. The
presence and distribution of sugar residues in the
oligosaccharide side chain of glycoconjugates were
investigated with the use of biotinylated lectins. The
pharynx and oesophagus of adult fishes showed the
presence of abundant secretory cells which synthesized a
large quantity of neutral, as well as sulphated and
sialylated glycoconjugates, with different cellular
combinations of them in the proximal and distal tract.
This may be related to the complex functions carried out
by this end of the gut in a marine euryaline fish.
Epithelia1 secretory cells were found in the developing
oesophagus during larval life (14 days) earlier than in
the stomach and intestine (34 days). The simple
columnar epithelium that lined the gastric mucosa of
adult fish synthesized a mixture of neutral and acidic
glycoconjugates, whereas during larval life it was shown
to contain neutral glycoconjugates only. The intestinal
goblet cells were shown to secrete both neutral and
acidic glycoconjugates, especially sulphated forms. The
adherent mucus gel of the gastric and intestinal mucosa
contained many sugar residues, as revealed by lectin
histochemistry. This work clearly demonstrates that the
quality of gut mucosubstances varies in different ages
and in regions of the fish alimentary canal. This is
possibly caused by changes in environmental conditions
and may in turn sustain functional alterations of the
digestive apparatus.
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