Publication: Targeted metabolic profiling of pomegranate polyphenols and urolithins in plasma, urine and colon tissues from colorectal cancer patients
Loading...
Date
2014-02-16
Authors
Núñez Sánchez, María Angeles ; García-Villalba, Rocío ; Monedero Saiz, Tamara ; García-Talavera, Noelia V. ; Gómez Sánchez, María B. ; Sánchez-Álvarez, Carmen ; García-Albert, Ana M. ; Rodríguez-Gil, Francisco J. ; Ruiz-Marín, Miguel ; Pastor Quirante, Francisco A. ; Martínez Díaz, Francisco ; Yáñez-Gascón, María J. ; González-Sarrías, Antonio ; Tomás-Barberán, Francisco A. ; Espín, Juan C.
item.page.secondaryauthor
item.page.director
Publisher
Wiley, Wiley-VCH Verlag
publication.page.editor
publication.page.department
DOI
https://doi.org/10.1002/mnfr.201300931
item.page.type
info:eu-repo/semantics/article
Description
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This document is the Published version of a Published Work that appeared in final form in Molecular Nutrition & Food Research. To access the final edited and published work see https://doi.org/10.1002/mnfr.201300931
Abstract
Scope: Urolithins are bioactive metabolites produced by the gut microbiota from ellagitannins (ETs) and ellagic acid (EA). We investigated whether urolithins could be detected in colon tissues from colorectal cancer (CRC) patients after pomegranate extract (PE) intake. Methods and results:
CRC patients (n = 52) were divided into controls and PEs consumers (900 mg/day for 15 days) before surgical resection. PEs with low (PE-1) and high (PE-2) punicalagin:EA ratio were administered. Twenty-three metabolites, but no ellagitannins, were detected in urine, plasma, normal (NT) or malignant (MT) colon tissues using UPLC-ESI-QTOF-MS/MS (UPLC, ultra performance liquid chromatography; QTOF, quadrupole TOF). Free EA, five EA conjugates, gallic acid and 12 urolithin derivatives were found in colon tissues. Individual and total metabolites levels were higher in NT than in MT, independently of the PE consumed. The maximal mean concentration (1671 ± 367 ng/g) was found in NT after consumption of PE-1 and the lowest concentration (42.4 ± 10.2 ng/g) in MT with PE-2. Urolithin A or isourolithin A were the main urolithins produced (54 and 46% patients with urolithin A or isourolithin A phenotype, respectively). High punicalagin content (PE-2) hampered urolithins formation. Conclusion: Significant levels of EA derivatives and urolithins are found in human colon tissues from CRC patients after consumption of pomegranate. Further studies are warranted to elucidate their biological activity.
publication.page.subject
Citation
Mol. Nutr. Food Res. 2014, 58, 1199–1211
item.page.embargo
Collections
Ir a Estadísticas
Sin licencia Creative Commons.