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Scaled-up biotechnological production of individualbetalains in a microbial system

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dc.contributor.authorGuerrero Rubio, María Alejandra
dc.contributor.authorLópez-Llorca, Rosalía
dc.contributor.authorHenarejos Escudero, Paula
dc.contributor.authorGandía Herrero, Fernando
dc.contributor.authorGarcía Carmona, Francisco
dc.contributor.departmentBioquímica y Biología Molecular "A"es
dc.date.accessioned2025-06-11T12:01:10Z
dc.date.available2025-06-11T12:01:10Z
dc.date.issued2019-07-04
dc.description© 2019, The Authors. This manuscript version is made available under the CC-BY-NC 4.0 license http://creativecommons.org/licenses/by-nc/4.0/. This document is the Published version of a Published Work that appeared in final form in Microbial Biotechnology. To access the final edited and published work see https://doi.org/10.1111/1751-7915.13452es
dc.description.abstractThe recent interest in plant pigment betalains asbioactive compounds and chemopreventive agents has led to the search for a reliable and scalable process to obtain them. The cloning of the novel andefficient enzyme 4,5-DOPA-extradiol dioxygenasefrom Gluconacetobacter diazotrophicus in an expres-sion vector, and the subsequent heterologous expression in Escherichia coli cultures has led to the start-up of a biotechnological production system of individual pigments. The aim of this study was to search for the optimal conditions for the production of betalamic acid in microbial factories and the scaled-up obtention of the derived pigments. Four different betaxanthins and two betacyanins were obtained after the addition of non-transformable amines and amino acids and their condensation with thebetalamic acid produced by the dioxygenase. The scaled-up obtention and purification of betalains improved the yields of the previous methodologies reaching quantities by up to 150 mg of pure compounds.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.citationMicrobial Biotechnology, 12, 993–1002
dc.identifier.doihttps://doi.org/10.1111/1751-7915.13452
dc.identifier.issnElectronic: 1751-7915
dc.identifier.urihttp://hdl.handle.net/10201/155803
dc.languageenges
dc.publisherWiley
dc.relationThis work was supported by the Spanish Ministry of Economy and Competitiveness (MEC-FEDER, Spain) (Projects AGL2014-57431 and AGL2017-86526) and by the ‘Programa de Ayudas a Grupos de Excelencia de la Region de Murcia, Fundacion Seneca, Agencia de Ciencia y Tecnologıa de la Region de Murcia’ (Project 19893/ GERM/15). M.A.G.-R. holds a contract financed by MEC-FEDER (Spain).es
dc.relation.publisherversionhttps://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1751-7915.13452
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución-NoComercial 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísicaes
dc.titleScaled-up biotechnological production of individualbetalains in a microbial systemes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublicationdb7a1a52-fec7-48eb-91e0-abe5e715b483
relation.isAuthorOfPublication77e02ea7-d1b7-4992-bc4a-c6e8c0cce1a8
relation.isAuthorOfPublication.latestForDiscoverydb7a1a52-fec7-48eb-91e0-abe5e715b483
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