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Scaled-up biotechnological production of individualbetalains in a microbial system

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Microbial Biotechnology - 2019 - Guerrero‐Rubio - Scaled‐up biotechnological production of individual betalains in a (1).pdf(1.21 MB)
Date
2019-07-04
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Authors
Guerrero Rubio, María Alejandra ; López-Llorca, Rosalía ; Henarejos Escudero, Paula ; Gandía Herrero, Fernando ; García Carmona, Francisco
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Publisher
Wiley
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Bioquímica y Biología Molecular "A"
DOI
https://doi.org/10.1111/1751-7915.13452
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Description
© 2019, The Authors. This manuscript version is made available under the CC-BY-NC 4.0 license http://creativecommons.org/licenses/by-nc/4.0/. This document is the Published version of a Published Work that appeared in final form in Microbial Biotechnology. To access the final edited and published work see https://doi.org/10.1111/1751-7915.13452
Abstract
The recent interest in plant pigment betalains asbioactive compounds and chemopreventive agents has led to the search for a reliable and scalable process to obtain them. The cloning of the novel andefficient enzyme 4,5-DOPA-extradiol dioxygenasefrom Gluconacetobacter diazotrophicus in an expres-sion vector, and the subsequent heterologous expression in Escherichia coli cultures has led to the start-up of a biotechnological production system of individual pigments. The aim of this study was to search for the optimal conditions for the production of betalamic acid in microbial factories and the scaled-up obtention of the derived pigments. Four different betaxanthins and two betacyanins were obtained after the addition of non-transformable amines and amino acids and their condensation with thebetalamic acid produced by the dioxygenase. The scaled-up obtention and purification of betalains improved the yields of the previous methodologies reaching quantities by up to 150 mg of pure compounds.
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Citation
Microbial Biotechnology, 12, 993–1002
URI
http://hdl.handle.net/10201/155803
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