Publication:
Correlation between the high expression levels of cancer-germline genes with clinical characteristics in esophageal squamous cell carcinoma

Simple item page
dc.contributor.authorChen, Xinfeng
dc.contributor.authorWang, Liping
dc.contributor.authorYue, Dongli
dc.contributor.authorLiu, Jinyan
dc.contributor.authorHuang, Lan
dc.contributor.authorYang, Li
dc.contributor.authorCao, Ling
dc.contributor.authorQin, Guohui
dc.contributor.authorLi, Anqi
dc.contributor.authorWang, Dan
dc.contributor.authorWang, Meng
dc.contributor.authorQi, Yu
dc.contributor.authorZhang, Bin
dc.contributor.authorvan der Bruggen, Pierre
dc.contributor.authorZhang, Yi
dc.date.accessioned2022-02-28T08:44:32Z
dc.date.available2022-02-28T08:44:32Z
dc.date.issued2017
dc.description.abstractAntigens encoded by cancer-germline genes are attractive targets for cancer immunotherapy. In this study, we aimed to evaluate the mRNA expression of cancer-germline genes, expression of the encoded proteins in patients with esophageal squamous cell carcinoma (ESCC) and their correlations with clinical characteristics. In addition, the effects of downregulation cancer-germline genes on ESCC cells were assessed in vitro. Our results showed that cancer-germline genes were frequently expressed in ESCC samples. The positive rates of in ESCC samples were: 87% of MAGEA3, 60% of MAGE-A4, 65% of MAGE-C2, and 20% of NY-ESO-1 at mRNA level. MAGE-A3 expression was associated with age, lymph node metastasis and tumor stage (all P<0.05), while MAGE-C2 expression was only associated with tumor stage (P<0.05). Furthermore, the MAGE-A3 expressing patients had a poorer overall survival (P<0.05). Multivariate analysis identified MAGE-A3 as an independent poor prognostic marker in ESCC. In vitro assay, ESCC cell lines treated with specific siRNAs to down-regulate MAGE-A3 and MAGE-C2 resulted in decreased colony-formation and migration ability (P<0.05). Epithelial marker E-cadherin was up-regulated in siRNA-MAGE-A3/C2 cells compared to controls, whereas mesenchymal markers Vimentin, N-cadherin and Slug were downregulated (all P<0.05), suggesting a role for MAGE-A3/C2 in ESCC metastasis through inducing epithelial-mesenchymal transition. The present study revealed that cancergermline genes and their encoded proteins were frequently expressed in ESCC tumor samples and were related to poor prognosis. Thus, cancer-germline genes may serve as useful biomarkers and potential targets for ESCC patientses
dc.formatapplication/pdfes
dc.format.extent11es
dc.identifier.citationHistology and Histopathology, Vol.32, nÂş8, (2017)
dc.identifier.doiDOI: 10.14670/HH-11-847
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/117428
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de BiologĂ­a Celular e HistologĂ­aes
dc.relationSin financiaciĂłn externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectEsophageal squamous cell carcinoma (ESCC)es
dc.subjectCancer-germline geneses
dc.subjectBiomarkerses
dc.subjectEpithelialmesenchymal transitiones
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - PatologĂ­a. Medicina clĂ­nica. OncologĂ­aes
dc.titleCorrelation between the high expression levels of cancer-germline genes with clinical characteristics in esophageal squamous cell carcinomaes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Chen-32-793-803-2017.pdf
Size:
8.63 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.39 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Histology and histopathology Vol.32, nÂş8 (2017)