Publication:
Correlation between the high expression levels of cancer-germline genes with clinical characteristics in esophageal squamous cell carcinoma

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Chen-32-793-803-2017.pdf(8.63 MB)
Date
2017
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Authors
Chen, Xinfeng ; Wang, Liping ; Yue, Dongli ; Liu, Jinyan ; Huang, Lan ; Yang, Li ; Cao, Ling ; Qin, Guohui ; Li, Anqi ; Wang, Dan ; Wang, Meng ; Qi, Yu ; Zhang, Bin ; van der Bruggen, Pierre ; Zhang, Yi
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Publisher
Universidad de Murcia. Departamento de BiologĂ­a Celular e HistologĂ­a
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DOI
DOI: 10.14670/HH-11-847
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info:eu-repo/semantics/article
Description
Abstract
Antigens encoded by cancer-germline genes are attractive targets for cancer immunotherapy. In this study, we aimed to evaluate the mRNA expression of cancer-germline genes, expression of the encoded proteins in patients with esophageal squamous cell carcinoma (ESCC) and their correlations with clinical characteristics. In addition, the effects of downregulation cancer-germline genes on ESCC cells were assessed in vitro. Our results showed that cancer-germline genes were frequently expressed in ESCC samples. The positive rates of in ESCC samples were: 87% of MAGEA3, 60% of MAGE-A4, 65% of MAGE-C2, and 20% of NY-ESO-1 at mRNA level. MAGE-A3 expression was associated with age, lymph node metastasis and tumor stage (all P<0.05), while MAGE-C2 expression was only associated with tumor stage (P<0.05). Furthermore, the MAGE-A3 expressing patients had a poorer overall survival (P<0.05). Multivariate analysis identified MAGE-A3 as an independent poor prognostic marker in ESCC. In vitro assay, ESCC cell lines treated with specific siRNAs to down-regulate MAGE-A3 and MAGE-C2 resulted in decreased colony-formation and migration ability (P<0.05). Epithelial marker E-cadherin was up-regulated in siRNA-MAGE-A3/C2 cells compared to controls, whereas mesenchymal markers Vimentin, N-cadherin and Slug were downregulated (all P<0.05), suggesting a role for MAGE-A3/C2 in ESCC metastasis through inducing epithelial-mesenchymal transition. The present study revealed that cancergermline genes and their encoded proteins were frequently expressed in ESCC tumor samples and were related to poor prognosis. Thus, cancer-germline genes may serve as useful biomarkers and potential targets for ESCC patients
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Esophageal squamous cell carcinoma (ESCC) , Cancer-germline genes , Biomarkers , Epithelialmesenchymal transition
Citation
Histology and Histopathology, Vol.32, nÂş8, (2017)
URI
http://hdl.handle.net/10201/117428
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Histology and histopathology Vol.32, nÂş8 (2017)
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