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Stigmasterol inhibits the progression of lung cancer by regulating retinoic acid-related orphan receptor C

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dc.contributor.authorDong, Yuan
dc.contributor.authorChen, Congcong
dc.contributor.authorChen, Chen
dc.contributor.authorZhang, Chunxia
dc.contributor.authorZhang, Lei
dc.contributor.authorZhang, Yan
dc.contributor.authorLi, Yongliang
dc.contributor.authorDong, Zhiqiang
dc.date.accessioned2023-02-03T09:44:50Z
dc.date.available2023-02-03T09:44:50Z
dc.date.issued2021
dc.description.abstractObjective. This study aims to investigate the role of stigmasterol in lung cancer. The study aims to investigate the role of stigmasterol in lung cancer and further explore its possible mechanisms. Methods. Cell Counting Kit-8 assay, 5-ethynyl-2- deoxyuridine (EdU), TUNEL and Flow cytometry were conducted to detect the proliferation and apoptosis of lung cancer cell lines. qRT-PCR and western blot were conducted to detect mRNA and protein levels of caspase-3 and caspase-9. In addition, Gene Ontology, STRING, SWISSMODEL, cellular thermal shift assay (CETSA) and Swiss Target Prediction were used to predict the targets of stigmasterol. Results. Behavioral studies showed that stigmasterol inhibited the proliferation and promoted the apoptosis of lung cancer cells. Further research revealed that retinoic acid-related orphan receptor C (RORC) directly targeted stigmasterol in lung cancer. Interestingly, rescue experiments indicated that RORC overexpression reversed the inhibitory effect of stigmasterol on lung cancer. Conclusion. In our study, we confirmed the functional role of the stigmasterol-RORC axis in lung cancer progression, which provides a latent target for lung cancer treatment.es
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dc.format.extent15es
dc.identifier.doihttps://doi.org/10.14670/HH-18-388
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/128145
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLung canceres
dc.subjectStigmasteroles
dc.subjectRetinoic acidrelated orphan receptor Ces
dc.subjectNetwork pharmacologyes
dc.subjectCaspasees
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleStigmasterol inhibits the progression of lung cancer by regulating retinoic acid-related orphan receptor Ces
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.36,nº12 (2021)