Publication: Stigmasterol inhibits the progression of lung cancer by regulating retinoic acid-related orphan receptor C
Authors
Dong, Yuan ; Chen, Congcong ; Chen, Chen ; Zhang, Chunxia ; Zhang, Lei ; Zhang, Yan ; Li, Yongliang ; Dong, Zhiqiang
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-388
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info:eu-repo/semantics/article
Description
Abstract
Objective. This study aims to investigate the
role of stigmasterol in lung cancer. The study aims to
investigate the role of stigmasterol in lung cancer and
further explore its possible mechanisms.
Methods. Cell Counting Kit-8 assay, 5-ethynyl-2-
deoxyuridine (EdU), TUNEL and Flow cytometry were
conducted to detect the proliferation and apoptosis of
lung cancer cell lines. qRT-PCR and western blot were
conducted to detect mRNA and protein levels of
caspase-3 and caspase-9. In addition, Gene Ontology,
STRING, SWISSMODEL, cellular thermal shift assay
(CETSA) and Swiss Target Prediction were used to
predict the targets of stigmasterol.
Results. Behavioral studies showed that stigmasterol
inhibited the proliferation and promoted the apoptosis of
lung cancer cells. Further research revealed that retinoic
acid-related orphan receptor C (RORC) directly targeted
stigmasterol in lung cancer. Interestingly, rescue
experiments indicated that RORC overexpression
reversed the inhibitory effect of stigmasterol on lung
cancer.
Conclusion. In our study, we confirmed the
functional role of the stigmasterol-RORC axis in lung
cancer progression, which provides a latent target for
lung cancer treatment.
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Citation
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