Sola Martínez, Rosa Alba

Person:
Sola Martínez, Rosa Alba

Name

Sola Martínez, Rosa Alba

publication.page.department

Universidad de Murcia. Departamento de Bioquímica y Biología Molecular"B" e Inmunología

Full item page

Search Results

Now showing 1 - 10 of 19

Open Access
Bacterial sirtuins overview: an open niche to explore
(Frontiers, 2021-10-26) Lozano Terol, Gema; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Ortega Retuerta, Álvaro; Sola Martínez, Rosa Alba; Bioquímica y Biología Molecular B e Inmunología
Restricted
Engineering of microbial cell factories for production of plant-based natural products
(Elsevier, 2021) Gallego Jara, Julia; Lozano Terol, Gema; Sola Martínez, Rosa Alba; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Bioquímica y Biología Molecular B e Inmunología; Facultades de la UMU::Facultad de Medicina
Biotechnology has become a promising alternative to produce highly valuable products. Production using microorganisms competes with chemical synthesis and extraction from natural sources, leading to cheaper and more sustainable production. To produce natural plant products using microorganisms, the development of molecular biology techniques that allow us to genetically and metabolically modify host microorganisms is essential. Thus many biotechnological processes have been developed to obtain terpenes, alkaloids, or polyphenols with interesting applications in the pharmaceutical, food, or cosmetic industries. The increase in our knowledge regarding the metabolism of host organisms, together with the continuous development of genetic and metabolic engineering techniques, will allow, in the coming years, biotechnology to be positioned as the main way to obtain high-value plant natural products.
Open Access
Data preprocessing workflow for exhaled breath analysis by GC/MS using open sources
(2020) Pastor Hernández, José María; Lozano-Terol, Gema; García-Marcos Álvarez, Luis Vicente; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Sola Martínez, Rosa Alba; Bioquímica y Biología Molecular B e Inmunología
The noninvasive diagnosis and monitoring of high prevalence diseases such as cardiovascular diseases, cancers and chronic respiratory diseases are currently priority objectives in the area of health. In this regard, the analysis of volatile organic compounds (VOCs) has been identified as a potential noninvasive tool for the diagnosis and surveillance of several diseases. Despite the advantages of this strategy, it is not yet a routine clinical tool. The lack of reproducible protocols for each step of the biomarker discovery phase is an obstacle of the current state. Specifically, this issue is present at the data preprocessing step. Thus, an open source workflow for preprocessing the data obtained by the analysis of exhaled breath samples using gas chromatography coupled with single quadrupole mass spectrometry (GC/MS) is presented in this paper. This workflow is based on the connection of two approaches to transform raw data into a useful matrix for statistical analysis. Moreover, this workflow includes matching compounds from breath samples with a spectral library. Three free packages (xcms, cliqueMS and eRah) written in the language R are used for this purpose. Furthermore, this paper presents a suitable protocol for exhaled breath sample collection from infants under 2 years of age for GC/MS.
Open Access
Regulation of the pyrimidine biosynthetic pathway by lysine acetylation of E. coli OPRTase
(2022-08-22) Lozano Terol, G.; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Martínez Vivancos, Adrián; Ortega Retuerta, Álvaro; Sola Martínez, Rosa Alba; Bioquímica y Biología Molecular B e Inmunología
Open Access
Dynamic Lysine Acetylation Disrupts Isocitrate Lyase Function and Enables Metabolic Optimisation
(Wiley, 2026-03-30) Martínez Vivancos, Adrián; Gomariz-Turpin, Beatriz; Ortega Retuerta, Álvaro; Lozano Terol, Gema; Sola Martínez, Rosa Alba; Gallego Jara, Julia; Diego Puente, Teresa de; Bioquímica y Biología Molecular B e Inmunología
Proteomic studies have suggested that Escherichia coli isocitrate lyase (ICL) undergoes multiple acetylation events, partially inhibiting its activity. However, the molecular basis of this regulation and the contribution of individual lysine residues had not been defined. This study demonstrates that acetylation of ICL in E. coli is acetyl-phosphate–dependent and reversible by the CobB deacetylase, establishing a key post-translational regulatory mechanism within the glyoxylate shunt. Site-specific acetylation at K13 and K308 inhibits ICL activity by destabilising the tetrameric assembly and rendering the protein more prone to degradation, whereas lysine-to-arginine substitutions at these positions alleviate this inhibition, enhancing carbon flux distribution, metabolic flexibility and biomass yield without the burden of plasmid-based overexpression. Leveraging this regulatory insight, a KR mutant bearing lysine-to-arginine substitutions at residues 13 and 308, engineered directly into the chromosomal aceA gene, maintained wild-type growth rates while reducing acetate overflow and improving metabolic balance during glucose depletion and acetate assimilation, leading to a 61% increase in lycopene production. These findings highlight regulatory-based metabolic engineering as a powerful strategy to optimise bioproduction and pave the way for extending this approach to other central metabolic enzymes to develop robust microbial cell factories for the sustainable synthesis of biofuels, biochemicals and high-value compounds.
Open Access
Bacterial sirtuins overview: an open niche to explore
(Frontiers Media, 2021-10-26) Lozano Terol, Gema; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Ortega Retuerta, Álvaro; Sola Martínez, Rosa Alba; Bioquímica y Biología Molecular B e Inmunología
Sirtuins are deacetylase enzymes widely distributed in all domains of life. Although for decades they have been related only to histones deacetylation in eukaryotic organisms, today they are considered global regulators in both prokaryotes and eukaryotes. Despite the important role of sirtuins in humans, the knowledge about bacterial sirtuins is still limited. Several proteomics studies have shown that bacterial sirtuins deacetylate a large number of lysines in vivo, although the effect that this deacetylation causes in most of them remains unknown. To date, only the regulation of a few bacterial sirtuin substrates has been characterized, being their metabolic roles widely distributed: carbon and nitrogen metabolism, DNA transcription, protein translation, or virulence. One of the most current topics on acetylation and deacetylation focuses on studying stoichiometry using quantitative LC-MS/MS. The results suggest that prokaryotic sirtuins deacetylate at low stoichiometry sites, although more studies are needed to know if it is a common characteristic of bacterial sirtuins and its biological significance. Unlike eukaryotic organisms, bacteria usually have one or few sirtuins, which have been reported to have closer common ancestors with the human Sirt5 than with any other class. In this work, in addition to carrying out an in-depth review of the role of bacterial sirtuins in their physiology, a phylogenetic study has been performed that reveals the evolutionary differences between sirtuins of different bacterial species and even between homologous sirtuins.
Open Access
Análisis del aire exhalado "off-line" para la búsqueda de biomarcadores de enfermedades respiratorias en la clínica pediátrica
(Universidad de Murcia, 2022-07-11) Sola Martínez, Rosa Alba; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Escuela Internacional de Doctorado
En el aire exhalado humano es posible distinguir una gran variedad de compuestos orgánicos volátiles (VOCs). Sin embargo, el conjunto de VOCs, “volatiloma”, presentes en el aire exhalado y su concentración en cada individuo pueden depender de diversos factores, como diferencias en el metabolismo, presencia de patologías o exposición a contaminantes ambientales, entre otros. Por todo ello, en los últimos años, el aire exhalado humano ha sido considerado una fuente prometedora de biomarcadores para el diagnóstico y la monitorización de numerosas patologías como enfermedades respiratorias de alta prevalencia. Asimismo, también se ha sugerido que el análisis del aire exhalado podría ser útil para controlar las exposiciones a contaminantes ambientales y para el seguimiento del estrés oxidativo. Una de las principales ventajas de este enfoque, es que se trata de una metodología no invasiva. La estrategia más extendida para la determinación de VOCs en el aliento humano es el análisis del aire exhalado fuera de línea u off-line mediante tecnologías basadas en espectrometría de masas. El objetivo principal de esta Tesis Doctoral ha sido aplicar y avanzar en el desarrollo del análisis del aire exhalado off-line para la búsqueda de biomarcadores de enfermedades respiratorias y/o atópicas en población pediátrica y en mujeres en edad fértil. Gran parte de la presente Tesis Doctoral se enmarca dentro del estudio NELA (Nutrition in Early Life and Asthma) que ha reclutado una cohorte de 738 parejas madre/hijo de la Región de Murcia desde la semana 20 de embarazo, con la finalidad de esclarecer el origen del asma durante la infancia y determinar qué factores influyen en su desarrollo. Así, en esta tesis se ha recogido y analizado las muestras de aire exhalado de las parejas de madres e hijos de la cohorte NELA a los 3 meses de edad del niño. En el Capítulo 3, se ha realizado una revisión sistemática de los estudios previos donde se ha aplicado el análisis del aire exhalado en población pediátrica. En el Capítulo 4, se expone el desarrollo de un flujo de trabajo de código abierto para el preprocesamiento de datos brutos obtenidos mediante espectrometría de masas. Además, en este capítulo se muestra un protocolo de recogida de aire exhalado para adultos y niños de corta edad, y un protocolo de análisis del aire exhalado off-line mediante TD-GC/q-MS (desorción térmica acoplada a cromatografía de gases-espectrometría de masas de cuadrupolo simple). En el Capítulo 5, se han identificado perfiles de VOCs en el aire exhalado de mujeres en edad fértil que permiten discriminar entre asmáticas con otras enfermedades atópicas coexistentes y no asmáticas. Asimismo, también se evaluó el impacto de la época del año cuando se realizaron las determinaciones de VOCs, sobre el proceso de descubrimiento de biomarcadores de enfermedades mediante el análisis del aire exhalado off-line, y se desarrollaron estrategias para afrontar los posibles sesgos introducidos. En el Capítulo 6, se estudió la relación entre los VOCs exhalados y la función pulmonar en lactantes sanos. En el Capítulo 7, se determinó la influencia de la exposición a la humedad en los hogares sobre los VOCs en el aire exhalado. Por último, en el Capítulo 8, se evaluó el efecto del almacenamiento de muestras de aire exhalado antes de su análisis mediante sistemas analíticos on-line, concretamente SESI-HRMS (ionización secundaria por electrospray-espectrometría de masas de alta resolución). En base a los resultados obtenidos, las principales conclusiones que se pueden extraer de la presente Tesis Doctoral son: 1) La introducción de las prácticas típicas del campo de la metabolómica es fundamental para alcanzar la traslación clínica del análisis del aire exhalado; 2) Se ha diseñado un protocolo para la recogida y análisis de muestras de aire exhalado mediante TD-GC/q-MS adecuado para población adulta y pediátrica; 3) Se ha desarrollado un flujo de trabajo de código abierto para el preprocesamiento de datos obtenidos en el análisis del aire exhalado off-line mediante TD-GC/q-MS; 4) El análisis del aire exhalado off-line mediante TD-GC/q-MS en mujeres en edad fértil permite diferenciar entre asmáticas con otras enfermedades atópicas coexistentes y no asmáticas (con o sin enfermedades atópicas); 5) Se ha caracterizado la varianza causada por los factores experimentales por implementación de ASCA, que combina el análisis de varianza (ANOVA) y el análisis de componentes simultáneos y se ha solventado el sesgo causado por la estación del año en la que se recolectaban las muestras de aliento; 6) Existe una asociación entre los VOCs exhalados y la función pulmonar en lactantes sanos; 7) La exposición prolongada a humedad en el hogar puede influir en el volatiloma exhalado, concretamente en los niveles del contaminante 2-etil-1-hexanol en mujeres en edad fértil y 8) Las muestras de aire exhalado pueden ser almacenadas durante horas en bolsas de muestreo de gas sin una pérdida determinante de intensidad de los compuestos, y posteriormente ser analizadas mediante SESI-HRMS.
Open Access
Engineering protein production by rationally choosing a carbon and nitrogen source using E. coli BL21 acetate metabolism knockout strains.
(Springer Nature, 2019-09-04) Lozano Terol, Gema; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Sola Martínez, Rosa Alba; Bioquímica y Biología Molecular B e Inmunología
Background: Escherichia coli (E. coli) is a bacteria that is widely employed in many industries for the production of high interest bio-products such as recombinant proteins. Nevertheless, the use of E. coli for recombinant protein production may entail some disadvantages such as acetate overfow. Acetate is accumulated under some culture conditions, involves a decrease in biomass and recombinant protein production, and its metabolism is related to protein lysine acetylation. Thereby, the carbon and nitrogen sources employed are relevant factors in cell host metabolism, and the study of the central metabolism of E. coli and its regulation is essential for optimizing the production of biomass and recombinant proteins. In this study, our aim was to fnd the most favourable conditions for carrying out recombinant protein production in E. coli BL21 using two diferent approaches, namely, manipulation of the culture media composition and the deletion of genes involved in acetate metabolism and Nε-lysine acetylation. Results: We evaluated protein overexpression in E. coli BL21 wt and fve mutant strains involved in acetate metabolism (Δacs, ΔackA and Δpta) and lysine acetylation (ΔpatZ and ΔcobB) grown in minimal medium M9 (inorganic ammonium nitrogen source) and in complex TB7 medium (peptide-based nitrogen source) supplemented with glucose (PTS carbon source) or glycerol (non-PTS carbon source). We observed a dependence of recombinant protein production on acetate metabolism and the carbon and nitrogen source employed. The use of complex medium supplemented with glycerol as a carbon source entails an increase in protein production and an efcient use of resources, since is a sub-product of biodiesel synthesis. Furthermore, the deletion of the ackA gene results in a fvefold increase in protein production with respect to the wt strain and a reduction in acetate accumulation. Conclusion: The results showed that the use of diverse carbon and nitrogen sources and acetate metabolism knockout strains can redirect E. coli carbon fuxes to diferent pathways and afect the fnal yield of the recombinant protein bioprocess. Thereby, we obtained a fvefold increase in protein production and an efcient use of the resources employing the most suitable strain and culture conditions.
Open Access
Data preprocessing workflow for exhaled breath analysis by GC/MS using open sources
(2020-12-15) Pastor Hernandez, J. M.; Lozano Terol, G.; García‑Marcos, Luis; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Sola Martínez, Rosa Alba; Bioquímica y Biología Molecular B e Inmunología
Open Access
Impact of the Expression System on Recombinant Protein Production in Escherichia coli BL21
(Frontiers Media, 2021-06-21) Lozano Terol, Gema; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Martínez Vivancos, Adrián; Sola Martínez, Rosa Alba; Bioquímica y Biología Molecular B e Inmunología
Recombinant protein production for medical, academic, or industrial applications is essential for our current life. Recombinant proteins are obtained mainly through microbial fermentation, with Escherichia coli being the host most used. In spite of that, some problems are associated with the production of recombinant proteins in E. coli, such as the formation of inclusion bodies, the metabolic burden, or the inefficient translocation/transport system of expressed proteins. Optimizing transcription of heterologous genes is essential to avoid these drawbacks and develop competitive biotechnological processes. Here, expression of YFP reporter protein is evaluated under the control of four promoters of different strength (PT7lac, Ptrc, Ptac, and PBAD) and two different replication origins (high copy number pMB10 and low copy number p15A). In addition, the study has been carried out with the E. coli BL21 wt and the ackA mutant strain growing in a rich medium with glucose or glycerol as carbon sources. Results showed that metabolic burden associated with transcription and translation of foreign genes involves a decrease in recombinant protein expression. It is necessary to find a balance between plasmid copy number and promoter strength to maximize soluble recombinant protein expression. The results obtained represent an important advance on the most suitable expression system to improve both the quantity and quality of recombinant proteins in bioproduction engineering.