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Muñoz Dávila, María José

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Muñoz Dávila, María José
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Universidad de Murcia. Departamento de Genética y Microbiología
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    Comparative evaluation of Vitek 2 identification and susceptibility testing of urinary tract pathogens directly and isolated from chromogenic media
    (Springer, 2013-01-12) Muñoz Dávila, María José; Roig, M.; Yagüe, G.; Blázquez, A.; Salvador, C.; Segovia, M.; Genética y Microbiología
    We evaluated the use of urine specimens for direct identification and antibiotic testing of urinary tract pathogens using the Vitek system. A total of 343 urine specimens from patients with suspected UTI were selected by pyuria and screened by Gram staining to detect bacteriuria. Of those, 132 were analysed after Gram staining, showing a high number of micro-organisms of a single morphological type. Direct susceptibility testing and identification were performed by using the Vitek system. Results were compared using the standard inoculation method based on the incubation of solid media. After sub-culture, 107 specimens grew a significant count of a single species and were used for the comparative analysis. The direct method correctly identified 88 isolates (82.3 %). When compared according to antibiotic susceptibility testing, the error rate was 2.4 % overall with 0.2 % very major, 0.4 % major and 1.8 % minor errors. 84.7 % of the Gram-negative bacilli had a complete susceptibility report in ≤8 h. This method offers the advantage of prompt processing and earlier reporting of complete results for positive urine specimens.
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    Prevalence of anal infection by human papillomavirus in men who have sex with men and its associated clinical and epidemiological factors
    (Sociedad Española de Quimioterapia, 2024-11-25) Muñoz Dávila, María José; Candel-Pérez, Carmen; García Villalba, Eva; Muñoz Pérez, María Ángeles; Genética y Microbiología
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    Comparative evaluation of Vitek 2 identification and susceptibility testing of Gram-negative rods directly and isolated from BacT/ALERT-positive blood culture bottles
    (Springer, 2011-07-28) Muñoz Dávila, María José; Yagüe, G.; Albert, M.; García-Lucas, T.; Genética y Microbiología
    The performance of Vitek 2 was evaluated for the identification and susceptibility testing of Gram-negative bacilli directly from positive blood cultures bottles. Direct inoculation of the positive blood cultures with the Vitek cards ID-GN and AST-NO58 was compared with the standard inoculation method based on the sub-culture of the positive blood culture to agar. A total of 142 blood cultures were included in the study; of those, 119 were from patients’ clinical samples, while 23 were artificially prepared with strains showing different mechanisms of resistance. A total of 136 (95.8%) strains were correctly identified to the species level, only 2 (1.4%) were mis-identified and 4 (2.8%) were not identified. Susceptibility results were available for all isolates tested against 17 antibiotics, thus, resulting in a total of 2,414 isolate/anti-microbial combinations. The error rate was 2.8% (67/2,414) overall; 0.6% (14/2,414) very major errors, 0.1% (3/2,414) major errors and 2.1% (50/2,414) minor errors. The direct method detected 88.5% (22/25) of the strains producing extended-spectrum beta-lactamases (ESBLs). The susceptibility agreement among the added strains with ESBL, AMPc hyperproduction, resistance to ceftazidime, carbapenems and cefepime was very high. Direct identification and susceptibility testing gave rapid and reliable results, reducing by 24 h the turnaround time of the microbiology laboratory.
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    Open Access
    Workflow for microbiological diagnosis of bacterial gastroenteritis combining a molecular assay as first-line with reflective stool culture
    (MDPI, 2023) Muñoz Dávila, María José; Candel-Pérez, C.; Vicente, M. R.; Piqueras, M.; Artero, J.M.; Genética y Microbiología
    Conventional microbiological methods for bacterial enteric disease diagnosis are time-consuming, labour intensive and provide low sensitivity. The aim of this study was to evaluate the results of a new diagnosis strategy which replaces traditional stool culture with a molecular detection using the BD MAX™ System (BD Life Sciences, Sparks, Maryland, United States) as first-line assay together with reflective culture. A total of 1.590 specimens were prospectively requested for stool culture. The molecular detection included the BD MAX enteric bacterial panel together with the BD MAX extended enteric bacterial panel (BDM GIP) performed simultaneously on the same stool specimen. In 18.8% of specimens (176 of the 936 valid samples) there was one or more than one target positive with the following percent positivity: 9.7% Campylobacter spp., 5.7% Salmonella spp., 1.3% Shiga toxin genes (stx1/stx2), 1.2% Shigella spp./enteroinvasive Escherichia coli (EIEC), 1% Yersinia enterocolitica, 1% Vibrio spp. (V. vulnificus/V. parahaemolyticus/V. cholerae), 0.3% Plesiomonas shigelloides, and 0.2% Enterotoxigenic E. coli (ETEC) enterotoxin LT/ST genes. Positive reflective stool culture noted a correlation of 69.5% with the molecular test, missing 23.9% and 15.4% in the cases of Campylobacter spp., and Salmonella spp., respectively. In conclusion, this clinical study demonstrated very good performance of the BDM GIP. The performance and ease of use may provide advantages to many laboratories, improving the detection of bacterial stool pathogens and time to reporting results.
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