Latorre Reviriego, Rafael Manuel

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Latorre Reviriego, Rafael Manuel

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Latorre Reviriego, Rafael Manuel

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Anatomía y Anatomía Patológica Comparadas

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Open Access
The heart of the killer whale: description of a plastinated specimen and review of the available literature
(MDPI, 2022-01-31) Latorre Reviriego, Rafael Manuel; Graïc, Jean Marie; Raverty, Stephen A.; Soria Gálvez, Federico; Cozzi, Bruno; López Albors, Octavio Miguel; Anatomía y Anatomía Patológica Comparada
The killer whale (Orcinus orca, Linnaeus, 1958) is the largest extant delphinid. Despite its worldwide distribution in the wild and in dolphinariums, its anatomy remains relatively poorly described. In the present study, we describe the detailed morphology of a plastinated killer whale heart. The gross description of the arteries and veins reaching the organ and its coronary vessels are reported. Additional endoscopy and CT (computed tomography) scanning were performed to provide extensive measurements of its parts. In many aspects, the killer whale heart conformed to other delphinid heart descriptions, including position, relative size and shape and specific features such as extensive papillary muscles, trabecular endocardium and trabecula septomarginalis. These characteristics are representative of the delphinid family, suggesting that its functions and capacities are similar to that of other, smaller, dolphins and help understand the conditions in which these predators exert their remarkable physical performance necessary for their survival.
Open Access
Organización morfológica e histoquímica de los distintos tipos de fibras que integran el músculo flexor carporradial del perro
(Universidad de Murcia, 2009-04-06) Latorre Reviriego, Rafael Manuel; Vázquez Autón, José María; Moreno Medina, Francisco; Gil Cano, Francisco; Departamentos y Servicios::Departamentos de la UMU::Anatomía y Anatomía Patológica Comparada
Mediante diferentes técnicas histoquímicas, se analizan los tipos de fibras de la musculatura esquelética (Mm. semitendinoso y flexor carporradial) de perros adultos y cachorros (0-65días). Tomando el músculo semitendinoso como patrón, se establecen los criterios para la correcta identificación de las distintas fibras. En base a la actividad mATPasa se reconocen 4 tipos diferentes, que denominamos: I, IIA, IIp y IIC. Con idéntica metodología y criterios de interpretación, se procede al estudio el M. flexor carporradial. Secciones transversales completas de este músculo obtenidas en criosotato a -20ºC, fueron analizadas desde el punto de vista histoquímico, procediéndose al estudio de los tipos de fibras y distribución de las mismas. Los resultados obtenidos indican que el M. flexor carporradial presenta dos cabezas, radial y cubital, cuya funcionalidad puede ser diferente. La cabeza radial se integra casi de forma exclusiva por fibras tipo I, mientras la cabeza cubital muestra una distribución en mosaico con presencia de fibras I, IIA y IIC. Las fibras IIp no fueron identificadas en ninguna de las dos cabezas. Los resultados parecen sugerir que el M. flexor carporradial del perro no solo participa en la protracción del miembro torácico (flexión del carpo), sino que desarrolla un importante papel en el aplomo del mismo. Los estudios llevados a cabo durante el periodo postnatal (0-65días), confirman la aparición gradual y progresiva de las fibras identificadas en animales adultos, aunque en distintos periodos de tiempo según el músculo y/o cabezas. Las técnicas de m-ATPasa permiten formular una nomenclatura específica para las fibras durante el desarrollo, que puede ser tomada como referencia para futuros estudios en estos periodos. Abstract: By jeans of different histochemical techniques, fibre types of skeletal muscle (Mm. semitendinosus and flexor carpi radialis) in adults and young dogs (0-65 days) were analysed. Histochemical criteria to the right identification of the different fibres were established by using semiteninosus muscle as control. According to their m-ATPase activity, we observed four different fibre types designated as I, IIA, IIp and IIC. To study the flexor carpi radialis muscle the same techniques and histochemical criteria were followed too. Cryostat whole cross serial sections were histochemically analysed, and the fibre types as well as their distribution studied. The results showed that flexor carpi radialis muscle has two heads, radial and cubital, probably with different functionality. Radial head was almost exclusively constituted by type I fibres; on the other hand, cubitl head exhibited mosaic pattern distribution, containing I, IIA and IIC fibres. None of the heads showed IIp fibre types. These findings suggest that flexor carpi radialis muscle in dog not only participates in the protraction of the forelimb (flexes carpal joint) but also takes an important part in its own aplomb. This study which was carried out throughout postnatal period (0-65 days) confirmed that occurrence of the different fibre types is gradual and progressive in time depending on muscle and / or head portion. mATPase techniques allow us the formulation of an specific nomenclature for the fibres during their development thus, it is recommended for further studies of muscle development.
Open Access
Órganos plastinados: recursos para una propuesta de innovación educativa STEM en Educación Secundaria
(Universidad de Murcia, Servicio de Publicaciones, 2023) Delgado-Ruiz, M.ª Carmen; Bernal-Sánchez, Rocío; Latorre Reviriego, Rafael Manuel; López Albors, Octavio Miguel
Los órganos plastinados son piezas anatómicas reales producidas en un laboratorio, imperecederas, carentes de toxicidad y que pueden usarse en cualquier escenario docente. En este trabajo se expone una experiencia educativa novedosa para el ámbito STEM basada en la utilización de órganos plastinados de origen animal. Se diseñó una propuesta didáctica innovadora para el 3er curso de ESO que fue evaluada en cuatro centros de Educación Secundaria durante un periodo de cuatro semanas. El diseño instruccional de la propuesta se apoyó en métodos activos con manipulación directa de los órganos plastinados y de forma complementaria una serie de recursos digitales en forma de vídeos, materiales para actividades gamificadas, cuestionarios y recursos educativos interactivos. Para evaluar la experiencia se utilizó un método mixto que incluyó una recogida de datos cuantitativos y cualitativos mediante cuestionarios y grupos focales con el profesorado y los estudiantes. Los resultados mostraron un alto índice de satisfacción de todos los agentes educativos implicados en la experiencia. Las principales limitaciones señaladas fueron la falta de tiempo, falta de adecuación al calendario y a la programación didáctica de los centros, la experimentación con solo uno de los segmentos del ámbito STEM y la falta de homogeneidad de las muestras participantes. A pesar de todo ello, se puede concluir que el uso de los órganos plastinados en educación secundaria ofrece amplias posibilidades para promover la innovación en educación STEM, a la vez que garantiza unos elevados índices de satisfacción del profesorado y del estudiantado.
Open Access
Tipos de fibras en el músculo esquelético del toro de lidia (bos taurus ibericus): estudio histoquímico y morfométrico.
(Murcia: Universidad de Murcia, Servicio de Publicaciones, 1997) Vázquez Autón, José María; Moreno Medina, Francisco; Gil Cano, Francisco; Latorre Reviriego, Rafael Manuel; López Albors, Octavio Miguel; Martínez Gomariz, Francisco; Ramírez Zarzosa, Gregorio José; Facultad de Veterinaria
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Device for collecting uterine fluid in vivo
(European Patent Office, 2024-07-03) Latorre Reviriego, Rafael Manuel; Canha Gouveia, Analuce; Anatomía y Anatomía Patológica Comparada; Facultades de la UMU::Facultad de Veterinaria
The present invention relates to a device for col-lecting uterine fluid, that can be used in vivo both in an-imals and humans without said use causing dilution ofthe uterine fluid components or damage to the endometrium. ------
Open Access
Museo Anatómico Veterinario de la Universidad de Murcia:Gestión de su información para la planificación,organización, educación y acción cultural
(Murcia: Universidad de Murcia, Servicio de publicaciones, 2000) Granado, J. D.; Chaín Navarro, Celia; Vázquez Autón, José María; Gil Cano, Francisco; Latorre Reviriego, Rafael Manuel; López Albors, Octavio Miguel; Orenes Hernández, Mariano; Ramírez Zarzosa, Gregorio José; Facultad de Veterinaria
Open Access
Anatomía veterinaria: experiencia metodológica en el marco del espacio europeo de educación superior
(Murcia, Universidad de Murcia, Servicio de Publicaciones, 2008) Vázquez Autón, José María; Ayala Florenciano, María Dolores; Gil Cano, Francisco; Latorre Reviriego, Rafael Manuel; López Albors, Octavio Miguel; Martínez Gomariz, Francisco; Ramírez Zarzosa, Gregorio José; Sánchez Collado, Cayetano; Facultad de Veterinaria
Open Access
Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant placental expression of PEG3 and LUM.
(BMC, 2021-02-15) París-Oller, E.; Navarro-Serna, S.; Soriano-Ubeda, C.; Lopes, J.S.; Matás, C.; Ruiz, S.; Cánovas, S.; Coy, P.; Romar, R.; Latorre Reviriego, Rafael Manuel; López Albors, Octavio Miguel; Fisiología; Anatomía y Anatomía Patológica Comparadas
Background: In vitro embryo production (IVP) and embryo transfer (ET) are two very common assisted reproductive technologies (ART) in human and cattle. However, in pig, the combination of either procedures, or even their use separately, is still considered suboptimal due to the low efficiency of IVP plus the difficulty of performing ET in the long and contorted uterus of the sow. In addition, the potential impact of these two ART on the health of the offspring is unknown. We investigated here if the use of a modified IVP system, with natural reproductive fluids (RF) as supplements to the culture media, combined with a minimally invasive surgery to perform ET, affects the output of the own IVP system as well as the reproductive performance of the mother and placental molecular traits. Results: The blastocyst rates obtained by both in vitro systems, conventional (C-IVP) and modified (RF-IVP), were similar. Pregnancy and farrowing rates were also similar. However, when compared to in vivo control (artificial insemination, AI), litter sizes of both IVP groups were lower, while placental efficiency was higher in AI than in RF-IVP. Gene expression studies revealed aberrant expression levels for PEG3 and LUM in placental tissue for C-IVP group when compared to AI, but not for RF-IVP group. Conclusions: The use of reproductive fluids as additives for the culture media in pig IVP does not improve reproductive performance of recipient mothers but could mitigate the impact of artificial procedures in the offspring. Keywords: Assisted reproductive technologies, Embryo transfer, In vitro embryo production, Placenta, Reproductive fluids
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Effects of pneumoperitoneum and body position on the morphology of the caudal cava vein analyzed by MRI and plastinated sections
(Springer Nature, 2012-10-24) Párraga Ros, Ester; López Albors, Octavio Miguel; Sánchez-Margallo, Fco; Moyano-Cuevas, J.L.; Latorre Reviriego, Rafael Manuel; Anatomía y Anatomía Patológica Comparada
Background Pneumoperitoneum and patient positioning are essential factors during laparoscopic surgical procedures. They cause hemodynamic and anatomical changes in several abdominal organs among which the caudal cava vein (CCV) is involved. Hemodynamic changes in this vein (decreased venous return) have been described in the porcine model, but how the vein morphology and size is affected at different abdominal levels is unknown. We sought to assess the morphological and morphometrical changes in the CCV of the pig caused by pneumoperitoneum and the reverse Trendelenburg position by in vivo magnetic resonance imaging (MRI). Methods Six pigs were scanned via MRI under four situations: S1, control (no pneumoperitoneum); S2, control in the reverse Trendelenburg position; S3, pneumoperitoneum (14 mmHg); and S4, pneumoperitoneum in the reverse Trendelenburg position. MRI and plastinated body sections were used to evaluate the topography, morphology and cross-sectional area of the CCV. Results Two portions of the CCV were differentiated: a prehepatic portion (located between the vertebral levels L1–T15) with flat and irregular morphology, and a hepatic portion (between T14–T11) that was almost rounded. The reverse Trendelenburg position caused an increase in the lumen affecting mainly the prehepatic portion, while pneumoperitoneum caused a decrease in the total vascular lumen, exerting a greater effect on the hepatic portion. The combination of both situations resulted in a further decrease in the vascular area and global morphological changes. Conclusions The pneumoperitoneum and reverse Trendelenburg position caused morphological and morphometrical changes in the prehepatic and hepatic portions of the CCV, which should assist in gaining a better understanding of the hemodynamic changes described in the literature.
Open Access
The histochemical profiles of fibre types in porcine skeletal muscle
(Murcia : F. Hernández, 2001) Vázquez, J.Mª; Moreno, F.; Gil Cano, Francisco; Latorre Reviriego, Rafael Manuel; López Albors, Octavio Miguel; Ramírez Zarzosa, Gregorio José
Using a variety of histochemical methods -mATPase staining after alkaline and acid preincubations, NADH-TR and a-MGPDH- we have investigated the fibre types in porcine skeletal muscle. The results reveal that four major fibre types -1, IIA, IIB and II*- can be separated histochemically in Longissimus lumborum muscle of Landrace pigs. The histochemical properties of the muscle fibre type 11* are very similar to that of type IIX described in other mammals. The existence of IIX fibres in pig muscle has been recently demonstrated by molecular biology techniques and our results validate the use of histochemistry (mATPase) as an easy methodology to differentiate the three fast myosins (type 11 fibres) in pig muscle.