Person: Peres Rubio, Camila
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PublicationOpen AccessEdU_Saliva_Horse. Learn, Discover. Innovate - Vídeos(2026-04) Contreras Aguilar, María Dolores; Tvarijonaviciute, Asta; López Jornet, María Pía; Cerón Madrigal, José Joaquín; Monkeviciene, Ingrida; Pina-Alburquerque, Jose A.; Lamy, Elsa; Vazquez, Mercedes; Peres Rubio, Camila; Martínez Subiela, Silvia; Medicina y Cirugía Animal; Muñoz, Héctor; Villanueva, Sergi; Facultades de la UMU::Facultad de VeterinariaMaterial educativo sobre colección de las muestras de saliva desarrollado durante el proyecto Material Digital Inclusivo sobre Ciencia de la Saliva para los Planes de Estudios de las Instituciones Europeas de Educación Superior (EdU_Saliva)- PublicationOpen AccessEdU_Saliva_Sheep. Learn, Discover. Innovate - Vídeos(2026-04) Llamas Amor, Eva; Tvarijonaviciute, Asta; López Jornet, María Pía; Cerón Madrigal, José Joaquín; Monkeviciene, Ingrida; Pina-Alburquerque, Jose A.; Lamy, Elsa; Vazquez, Mercedes; Peres Rubio, Camila; Martínez Subiela, Silvia; Medicina y Cirugía Animal; Muñoz, Héctor; Villanueva, SergiMaterial educativo sobre colección de las muestras de saliva desarrollado durante el proyecto Material Digital Inclusivo sobre Ciencia de la Saliva para los Planes de Estudios de las Instituciones Europeas de Educación Superior (EdU_Saliva)
- PublicationOpen AccessEdU_Saliva_Baby. Learn, Discover. Innovate - Vídeos(2026-04) López Jornet, María Pía; Contreras Aguilar, María Dolores; Peres Rubio, Camila; Franco Martínez, Lorena; Tvarijonaviciute, Asta; Cerón Madrigal, José Joaquín; Monkeviciene, Ingrida; Pina-Alburquerque, Jose A.; Lamy, Elsa; Vazquez, Mercedes; Martínez Subiela, Silvia; Medicina y Cirugía Animal; Muñoz, Héctor; Villanueva, Sergi; Facultades de la UMU::Facultad de VeterinariaMaterial educativo sobre colección de las muestras de saliva desarrollado durante el proyecto Material Digital Inclusivo sobre Ciencia de la Saliva para los Planes de Estudios de las Instituciones Europeas de Educación Superior (EdU_Saliva)
- PublicationOpen AccessEdU_Saliva_Elderdly. Learn, Discover. Innovate - Vídeos(2026-04) López Jornet, María Pía; Tvarijonaviciute, Asta; Cerón Madrigal, José Joaquín; Monkeviciene, Ingrida; Pina-Alburquerque, Jose A.; Lamy, Elsa; Vazquez, Mercedes; Peres Rubio, Camila; Martínez Subiela, Silvia; Medicina y Cirugía Animal; Muñoz, Héctor; Villanueva, Sergi; Facultades de la UMU::Facultad de VeterinariaMaterial educativo sobre colección de las muestras de saliva desarrollado durante el proyecto Material Digital Inclusivo sobre Ciencia de la Saliva para los Planes de Estudios de las Instituciones Europeas de Educación Superior (EdU_Saliva)
- PublicationOpen AccessEdU_Saliva_Dog. Learn, Discover. Innovate - Vídeos(2026-04) Tvarijonaviciute, Asta; Cañadas Vidal, Esmeralda; Carrillo Sánchez, J. D.; López Jornet, María Pía; Cerón Madrigal, José Joaquín; Monkeviciene, Ingrida; Pina-Alburquerque, Jose A.; Lamy, Elsa; Vazquez, Mercedes; Peres Rubio, Camila; Martínez Subiela, Silvia; Medicina y Cirugía Animal; Muñoz, Héctor; Villanueva, Sergi; Facultades de la UMU::Facultad de VeterinariaMaterial educativo sobre colección de las muestras de saliva desarrollado durante el proyecto Material Digital Inclusivo sobre Ciencia de la Saliva para los Planes de Estudios de las Instituciones Europeas de Educación Superior (EdU_Saliva)
- PublicationOpen AccessEdU_Saliva_Pig. Learn, Discover. Innovate - Vídeos(2026-04) Llamas Amor, Eva; Tvarijonaviciute, Asta; López Jornet, María Pía; Cerón Madrigal, José Joaquín; Monkeviciene, Ingrida; Pina-Alburquerque, Jose A.; Lamy, Elsa; Vazquez, Mercedes; Peres Rubio, Camila; Martínez Subiela, Silvia; Medicina y Cirugía Animal; Muñoz, Héctor; Villanueva, Sergi; Facultades de la UMU::Facultad de VeterinariaMaterial educativo sobre colección de las muestras de saliva desarrollado durante el proyecto Material Digital Inclusivo sobre Ciencia de la Saliva para los Planes de Estudios de las Instituciones Europeas de Educación Superior (EdU_Saliva)
- PublicationOpen AccessSeminal plasma antioxidants are related to sperm cryotolerance in the horse(MDPI, 2022-06-28) Catalán, Jaime; Yánez Ortiz, Iván; Tvarijonaviciute, Asta; González Aróstegui, Luis Guillermo; Peres Rubio, Camila; Barranco Cascales, Isabel; Yeste, Marc; Miró, Jordi; Medicina y Cirugía AnimalThe objective of this study was to determine the relationship of enzymatic (superoxide dismutase, SOD; glutathione peroxidase, GPX; catalase, CAT; and paraoxonase type 1, PON1) and non-enzymatic antioxidants (measured in terms of: Trolox equivalent antioxidant capacity, TEAC; cupric-reducing antioxidant capacity, CUPRAC; and ferric-reducing ability of plasma, FRAP), as well as the oxidative stress index (OSI) in seminal plasma (SP) with the resilience of horse sperm to freeze-thawing. Twenty-one ejaculates (one per individual) were collected and split into two aliquots: the first was used to harvest the SP and assess the activity levels of antioxidants and the OSI, and the second one was cryopreserved. The following post-thaw sperm quality parameters were evaluated: sperm motility, plasma membrane and acrosome integrity, mitochondrial membrane potential, intracellular levels of reactive oxygen species (ROS), and plasma membrane lipid disorder. Based on post-thaw total motility (TM) and plasma membrane integrity (SYBR14+/PI−), ejaculates were hierarchically (p < 0.001) clustered into two groups of good (GFE) and poor (PFE) freezability. The SP activity levels of PON1, SOD, and TEAC were higher (p < 0.05) in GFE than in PFE, showing a positive relationship (p < 0.05) with some sperm motility parameters and with plasma membrane (PON1 and TEAC) and acrosome (SOD and TEAC) integrity. In contrast, OSI was higher (p < 0.05) in the SP of PFE than in that of GFE, and was negatively correlated (p < 0.05) to some sperm motility parameters and to plasma membrane and acrosome integrity, and positively (p < 0.05) to the percentage of viable sperm with high plasma membrane lipid disorder. In conclusion, enzymatic (PON1 and SOD) and non-enzymatic (TEAC) antioxidants of SP are related to horse sperm cryotolerance. In addition, our results suggest that PON1 could be one of the main antioxidant enzymes involved in the control of ROS in this species. Further investigation is needed to confirm the potential use of these SP-antioxidants and OSI to predict sperm cryotolerance in horses.
- PublicationOpen AccessProtective role of extracellular vesicles against oxidative DNA damage(BioMed Central, 2025-03-13) Ribas Maynou, Jordi; Parra, Ana; Martínez Díaz, Pablo; Peres Rubio, Camila; Lucas Arjona, Xiomara; Yeste, Marc; Roca, Jordi; Barranco Cascales, Isabel; Medicina y Cirugía Animal; Facultad de VeterinariaBackground: Oxidative stress, a source of genotoxic damage, is often the underlying mechanism in many functional cell disorders. Extracellular vesicles (EVs) have been shown to be key regulators of cellular processes and may be involved in maintaining cellular redox balance. Herein, we aimed to develop a method to assess the effects of EVs on DNA oxidation using porcine seminal plasma extracellular vesicles (sEVs). Results: The technique was set using a cell-free plasmid DNA to avoid the bias generated by the uptake of sEVs by sperm cells, employing increasing concentrations of hydrogen peroxide (H2O2) that generate DNA single-strand breaks (SSBs). Because SSBs contain a free 3'-OH end that allow the extension through quantitative PCR, such extension -and therefore the SYBR intensity- showed to be proportional to the amount of SSB. In the next stage, H2O2 was co-incubated with two size-differentiated subpopulations (small and large) of permeabilized and non-permeabilized sEVs to assess whether the intravesicular content (IC) or the surface of sEVs protects the DNA from oxidative damage. Results obtained showed that the surface of small sEVs reduced the incidence of DNA SSBs (P < 0.05), whereas that of large sEVs had no impact on the generation of SSBs (P > 0.05). The IC showed no protective effect against DNA oxidation (P > 0.05). Conclusions: Our results suggest that the surface of small sEVs, including the peripheral corona layer, may exert a protective function against alterations that are originated by oxidative mechanisms. In addition, our in vitro study opens path to detect, localize and quantify the protective effects against oxidation of extracellular vesicles derived from different fluids, elucidating their function in physiopathological states.
- PublicationOpen AccessEdU_Saliva_Cat. Learn, Discover. Innovate - Vídeos(2026-04) Cañadas Vidal, Esmeralda; Carrillo Sánchez, J. D.; López Jornet, María Pía; Cerón Madrigal, José Joaquín; Monkeviciene, Ingrida; Pina-Alburquerque, Jose A.; Lamy, Elsa; Vazquez, Mercedes; Peres Rubio, Camila; Martínez Subiela, Silvia; Tvarijonaviciute, Asta; Medicina y Cirugía Animal; Muñoz, Héctor; Villanueva, Sergi; Facultades de la UMU::Facultad de VeterinariaMaterial educativo sobre colección de las muestras de saliva desarrollado durante el proyecto Material Digital Inclusivo sobre Ciencia de la Saliva para los Planes de Estudios de las Instituciones Europeas de Educación Superior (EdU_Saliva)
- PublicationOpen AccessEffects of Commercial Antioxidants in Feed on Growth Performance and Oxidative Stress Status ofWeaned Piglets(MDPI AG, 2021-01-21) Orengo Femenia, Juan; Hernández Ruipérez, Fuensanta; Martínez Miró, Silvia; Sánchez Parra, Cristian Jesús; Peres Rubio, Camila; Madrid Sánchez, Josefa; Producción AnimalThis work aimed to evaluate the effect of adding two different commercial antioxidants (AOX) products to pre-starter and starter diets using low vitamin E (Vit E as DL- -tocopheryl acetate) levels on the growth performance and oxidative stress of piglets for the first six weeks post-weaning (PW). They were sorted by initial body weight (BW: 6.175 0.931 kg) and randomly allotted to four dietary treatments (with six replicates per treatment): a positive control (PC) and a negative control (NC) diet, with normal and low dose of vitamin E (80 and 15 mg kg1, respectively), both without AOX; the other two experimental diets with a low dose of vitamin E (LVE) plus LOXIDAN VD100 (LVE + AOX1) or LOXIDAN E Ros (LVE + AOX2). Growth data were recorded, and blood samples were taken, at the beginning (day 0) and at the end of each feeding period: pre-starter and starter (at days 14 and 42, respectively). No differences among dietary treatments were found with respect to growth performance in the pre-starter period (p 0.05). However, at the end of the starter period, a lower BW was found in piglets fed the NC diet compared to the other dietary treatments. Differences in daily gain and feed conversion ratio were also found either for the starter period or when the whole period was considered (p < 0.05), whereby piglets fed PC or LVE diets supplemented with AOX showed better growth performance compared to piglets fed the NC diet. Regarding Vit E ( -tocopherol) serum levels, there were no differences among treatments at day 0; but the serum values of this vitamin decreased in LVE diets at 14 and 42 days, but not in the PC. On day 42, the highest levels of -tocopherol in liver were also found in piglets fed PC (p < 0.05). Nevertheless, in general, from a metabolic point of view and after checking the serum biochemical profile of piglets, there were no differences in other oxidative stress markers (p 0.05). The results showed that the AOX products used were able to compensate for the lower Vit E supply with respect to growth performance in the starter phase. The use of AOXs or usual levels of Vit E in feed constitutes a key factor in achieving optimal growth performance of piglets in the PW period.
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