Person:
Abril Parreño, Laura

Profile Picture
Name
Abril Parreño, Laura
publication.page.department
Universidad de Murcia. Departamento de Fisiología
Repository logoRepository logo

Filters

20251
1
1
Reset filters

Settings

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    Publication
    Open Access
    Deciphering differences in DNA methylation and transcriptome profiles of oocytes from pigs with high and low developmental competence
    (Oxford University Press, 2025-06-03) Abril Parreño, Laura; Lopes, Jordana S.; Romero Aguirregomezcorta, Jon; Galvao, Antonio; Kelsey, Gavin; Coy Fuster, Pilar; Fisiología
    In vitro maturation (IVM) is a critical step in animal in vitro embryo production, yet oocytes matured in vitro often exhibit lower developmental competence than their in vivo counterparts. However, the molecular mechanisms behind this observation remain unclear. This study investigated the gene expression and DNA methylation profiles in porcine oocytes with different developmental competencies. To study these differences, we used as model oocytes from prepubertal gilts (IVM) and sows (in vivo matured) and assessed their developmental competence up to the blastocyst stage. We also examined their gene expression and DNA methylation profiles at single-cell resolution using RNA-sequencing and bisulfite-sequencing. Oocytes were obtained by aspiration of either ovarian follicles between 3-6mm diameter, and the subsequent IVM, or ovarian follicles from 8-10 mm diameter, with no need for maturation (in vivo matured oocytes). Cleavage rates (58.2±3.0 and 45.7±4.4) and blastocyst rates (31.4±3.7 and 47.5±6.6) for IVM and in vivo groups differed significantly. Using the in vivo group as a reference, IVM oocytes had 1297 down-regulated and 476 up-regulated differentially expressed genes (DEGs), with up-regulated DEGs associated with organelle organization and cell cycle processes, and down-regulated genes involved in protein synthesis and metabolomic processes. While global DNA methylation levels were similar between groups, a few differentially methylated regions were found in CpG islands, promoters, and coding regions. Our integrative analysis identified key methylated regions and genes that distinguish each group, suggesting that both donor age and maturation conditions significantly influence gene expression regulation in oocytes with different developmental competencies.
Ir a Estadísticas