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Contreras de Vera, Antonio

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Contreras de Vera, Antonio
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Universidad de Murcia. Departamento de Sanidad Animal
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    Isolation of Mycoplasma auris from milk of goats with clinical mastitis
    (Elsevier, 2020-03-07) García-Galán Pérez, Ana; García Romero, Edgar; Sánchez López, Antonio; Corrales Romero, Juan Carlos; Contreras de Vera, Antonio; Fé Rodríguez, David Christian de la; Sanidad Animal; Facultades de la UMU::Facultad de Veterinaria
    The unexpected presence of Mycoplasma auris was evidenced in mastitis samples collected from a dairy goat herd under surveillance programmes of contagious agalactia (CA), a serious disease affecting Mediterranean small ruminant herds. Thus, control programs based on permanent analysis of milk from bulk tank and clinical mastitis, as well as ear swabs, are currently conducted in affected areas. In order to analyze the presence of CAcausing species, three, 11 and 18 of these samples were respectively collected from the same goat herd. Mycoplasmas were isolated in four milk samples from clinical mastitis and two ear swabs samples and no other pathogen was detected. The presence of CA-causing mycoplasmas was discarded by PCR. However, M. auris was identified in all the positive samples after the amplification and posterior PCR product sequencing of a partial fragment of their 16S-ribosomal ARN gene. As far as we know, these are the first isolations of M. auris from milk of goats with clinical mastitis.
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    Testicular ultrasound patterns, sperm quality, seminal microbiota, caprine arthritis encephalitis virus and Coxiella burnetii infection in bucks
    (Elsevier, 2026-04-25) Bailón-Larrañaga, N.; Gomis, J.; Contreras de Vera, Antonio; Toledo-Perona, R.; Toquet, M.; Reyes, L.E.; Quereda, J.J.; Gonzalez-Torres, P.; Gñpmez-Marín, A.; Sanidad Animal
    This study assesses the frequency and severity of ultrasound-detected testicular alterations across age groups (young and adult) in 219 bucks. Sperm quality and testicular echotexture were evaluated, and their associations with reproductive pathogens (Coxiella (C.) burnetii, caprine arthritis encephalitis virus (CAEV), Chlamydia (C.) abortus and Mycoplasma (M.) agalactiae) were analyzed. Additionally, qPCR was also performed on ejaculates from seropositive bucks. Seminal microbiota was characterized in three groups: 1 (seronegative without lithiasis), 2 (seropositive with lithiasis), and 3 (qPCR-CAEV-positive). A 68.5% of bucks showed testicular microlithiasis (50.2% bilateral and 18.2% unilateral). Moreover, frequency of bilateral lesions differed according to age, being higher in adults, who also showed larger microlithiasis area (P < 0.05). Age was negatively correlated with sperm concentration (P < 0.05). Seropositivity was 35.8% for C. burnetii and 58.8% for CAEV. Adults showed higher CAEV seropositivity (P < 0.001), and 4.2% of ejaculates tested qPCR positive for this pathogen. Bacterial richness was greater (P < 0.001) in group 2 than in group 1. Firmicutes dominated groups 1 and 2, whereas Fusobacteriota prevailed in group 3. The most frequent genus in all three experimental groups was Oceanivirga, with higher abundance in group 1 than in group 2 (P < 0.05). These findings suggest that ejaculate microbiota may be influenced by ultrasound abnormalities and exposure to CAEV and C. burnetii, supporting the use of testicular ultrasound to detect bucks prone to reproductive problems and associated health risks, while also highlighting the need to include males in CAE and Q fever control programs.
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