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Pérez Silva, Amparo

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Pérez Silva, Amparo
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Universidad de Murcia. Departamento de Dermatología, Estomatología,Radiología y Medicina Física
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  • Publication
    Open Access
    Estudio comparativo del esmalte sano e hipomineralizado de dientes temporales: estudio piloto
    (Arán Ediciones , 2025-02-13) Serna Muñoz, Clara; Botella Pastor, Mar; Gómez Ríos, Inmaculada; Pérez Silva, Amparo; Ortiz Ruiz, Antonio José; Dermatología, Estomatología, Radiología y Medicina Física; Facultad de Medicina
    Introducción: el esmalte dental, compuesto principalmente por hidroxiapatita puede presentar hipomineralización, un defecto cualitativo observado tanto en dentición permanente (MIH) como temporal (DMH o HSPM). Objetivo: el objetivo de este estudio fue realizar un estudio macroscópico, microscópico y de composición elemental del esmalte de dientes sanos e hipomineralizados. Material y métodos: este estudio comparó dientes temporales sanos e hipomineralizados mediante observación macroscópica, microscopia electrónica de barrido (SEM) y espectroscopía de rayos X de energía dispersiva (EDX). Resultados: se encontraron diferencias en morfología, microestructura presentando los dientes con HSPM un esmalte desestructurado, poroso y fracturado. Con respecto a la composición química presentan significativamente menor contenido de Ca (14,38 vs 29,45) y P (8,89 vs 15,13), y mayor de C (30,79 vs 12,37). Conclusiones: el esmalte hipomineralizado presentó unas características macro- y microscópicas diferentes al esmalte sano, así como un menor contenido en Ca y P y un ratio Ca/P reducida.
  • Publication
    Open Access
    Knowledge, practices, and attitudes towards silver diamine fluoride therapy among dentists and students in south-eastern Spain
    (MDPI, 2025-01-02) Serna Muñoz, Clara; Lucas-Porras, Marina; Martínez Beneyto, Yolanda; Pérez Silva, Amparo; Ibáñez López, Francisco Javier; Ortiz Ruiz, Antonio José; Dermatología, Estomatología, Radiología y Medicina Física; Facultad de Medicina
    The use of silver diamine fluoride (SDF) has increased in recent years for the management of caries lesions in children and adults. The aim of this study is to determine the level of knowledge and the attitude of Spanish dentists (GDPs) and final-year dental students (DSs) regarding the use of SDF. Methods: A cross-sectional survey (questionnaire) was carried out aimed at final-year dental students (DSs) (n = 43) and registered dentists (GDPs) (n = 1050) in the autonomous community of the region of Murcia (Spain). Results: the response rates were GDPs 7.7% (n = 81) and DSs 84.5% (n = 38). Only 20.98% of GDP respondents reported having been trained on SDF versus 100% of DSs. Significant differences were observed between the groups (p < 0.05). While 94.7% of the students were aware of the indications for the use of SDF, only 56.8% of the general dentists reported it. Similarly, for hypersensitivity treatment, 71.1% of the students were informed versus 40.7% of the general dentists, and indications for paediatric patients, 100% for the DS group and 59.3% in GDPs. In adult patients, indications vary from GDPs’ (50%) to DSs’ (25.9%) responses. About 94.7% of DSs know the advantages of use and only 50.6% of GDPs. Both groups showed reluctance to use SDF in esthetic zones, with greater acceptance in non-esthetic areas (p < 0.05). In practice, fewer GDPs (27.16%) and DSs (23.68%) had applied SDF, reflecting a gap between knowledge and implementation. Conclusions: Dental students had a significantly higher level of knowledge, a situation that evidences the high level of education and training in the curricular guides of the universities.
  • Publication
    Open Access
    Time-dependent adhesion and fluoride release of resin-modified glass ionomer cements on demineralized enamel, sound enamel and dentine
    (MDPI, 2025-10-11) Gómez Ríos, Inmaculada; Ortiz Ruiz, Antonio José; Martínez Beneyto, Yolanda; Cabello, Inmaculada; Valverde Rubio, Pilar; Cereceda Villaescusa, Pilar; Pérez Silva, Amparo; Dermatología, Estomatología, Radiología y Medicina Física; Facultad de Medicina
    Background: The treatment of cavitated lesions has evolved with minimally invasive dentistry (MID), whereby we can leave demineralized enamel that could potentially be remineralizable with the use of materials such as resin-modified glass ionomer cements (RMGICs) that allow these lesions to be repaired and remineralized while removing less tooth tissue. The aim of our study was to compare the influence of aging on adhesion to sound enamel, demineralized enamel, and the healthy dentin of five RMGICs (Vitremer®, ACTIVA BioACTIVE Restorative, Riva LC, Ionolux®, and GC Fuji II LC) and fluoride release. There are currently no studies on adhesion in demineralized enamel. Method: A total of 1035 bovine incisors were analyzed in 45 groups of 23 teeth each. The groups were established based on three factors: time (24 h, 1 month, and 3 months); substrate (sound enamel, demineralized enamel, and healthy dentin); and type of material. In each group, 20 samples underwent shear bond strength (SBS) and fracture type analysis. Adhesive interfaces were observed in three samples from each group using field emission scanning electron microscopy (FESEM). Daily and cumulative fluoride release rates were calculated. Results: Adhesion improved over time on both demineralized and sound enamel. ACTIVA BioACTIVE Restorative had the highest SBS values (33.63 ± 10.69 MPa), and Vitremer® had the lowest (4.10 ± 4.63). Most fractures were adhesive. Vitremer® and Ionolux® showed the highest daily and cumulative fluoride release rates (Vitremer daily (24 h): 225.30 ± 26.28 ppm/g; Vitremer cumulative (30 days): 635.99 ± 305.38 ppm/g; Ionolux daily (24 h): 207.59 ± 48.43 ppm/g; Ionolux cumulative (30 days): 501.21 ± 138.71 ppm/g) and ACTIVA BioACTIVE Restorative showed the lowest (ACTIVA daily (24 h): 10.50 ± 0.85; ACTIVA cumulative (30 days): 39.10 ± 2.16). Conclusions: ACTIVA BioACTIVE Restorative was the material with the best adhesion values on all substrates and at all times, but it showed the lowest fluoride release rates.
  • Publication
    Open Access
    Application of machine learning for data analysis in paediatric dentistry: a systematic review
    (SIOI, Italian Society of Paediatric Dentistry, 2025-05) Gómez Ríos, Inmaculada; Saura López, Virginia; Pérez Silva, Amparo; Serna Muñoz, Clara; Ortiz Ruiz, Antonio José; Dermatología, Estomatología, Radiología y Medicina Física; Facultad de Medicina
    Aim: The study aims to assess whether the application of machine learning (ML) for database analysis enhances the approach to oral diseases in the paediatric population. Materials: Dental caries affects 514 million children worldwide. Artificial intelligence (AI), particularly ML, has seen increased utilisation in medicine and dentistry, handling data beyond human capacity to discern patterns and make predictions. PubMed, Web of Science, Scopus, and Lilacs databases were searched. Topics covered include the impact of oral health on adolescents' quality of life, predictors of early childhood caries and of the need of second treatment under deep sedation, and the effectiveness of preventive dental services. Methods: Twenty articles meeting eligibility criteria were analyzed for quality using the QUADAS-2 scale. The systematic review adhered to the PRISMA statement, yielding 20 articles out of 1945 initially screened. Fourteen articles focused on caries prediction, highlighting socio-demographic, behavioural, and biological predictors. ML analysis revealed that children with early caries lesions incur higher costs for insurers, with those receiving sealants and fluoride demonstrating greater cost savings. Conclusion: ML algorithms can identify patterns in large datasets, enhancing approaches to paediatric oral diseases. Their integration into research and educational programs is recommended. Methodological guidelines and quality scales specific to such studies are necessary for improved scientific evidence.
  • Publication
    Restricted
    Fluoride levels in saliva after the application of fluoride varnishes in a preventive oral health program in pregnant women: a randomized controlled trial
    (Wiley, 2025-03-11) Fernández Pizarro, Iciar; Ortiz Ruiz, Antonio José; Serna Muñoz, Clara; Pérez Silva, Amparo; Ibáñez López, Francisco Javier; Gallego Reyes, Sandra; Martínez Beneyto, Yolanda; Dermatología, Estomatología, Radiología y Medicina Física; Facultad de Medicina
    Purpose: Gestation is a time in women's lives when many physiological changes occur that have systemic and oral repercussions, especially in the periodontium. The aim of the study is to determine the oral health status, plaque index, oral health related quality of life, and concentration of fluoride in saliva, after the application of fluorinated varnishes, of pregnant women participating in a preventive oral health program. Material and methods: A randomized clinical trial was carried out on pregnant patients involved in an oral health program. Data was collected on socio-demographic aspects, hygiene habits, beliefs, epidemiological indexes such as Decayed, Missing, and Filled Teeth (DMFT); International Caries Detection and Assessment System (ICDAS); Community Periodontal Index (CPI); Caries Management by Risk Assessment (CAMBRA); and the Oral Health Related Quality of Life Index (OHIP-14). Three reviews were carried out during the gestation period (Day 1, 15, and 30). The fluoride varnishes applied in the study were Clinpro White Varnish, Profluorid Varnish, and MI Varnish. Results: A total of 89 women were evaluated. Most patients were Spanish and married, with a mean age of 34.3. The mean DMFT was 7.2 (± 4.51), the plaque index was 52%, and there was a low risk of caries. A poorer quality of life was linked to an increased risk of caries, high DMFT values, high ICDAS codes, and high plaque index. The concentration of fluoride in saliva immediately after the application with MI Varnish reaches values of fluor concentration 238.51 ± 151.87 much higher than the other varnishes (p < 0.05); however, after 30 days the values were reduced without significant differences for the three study groups Clinpro White Varnish (0.21 ± 0.29), MI Varnish (0.34 ± 0.34) and Profluorid Varnish (0.16 ± 0.07). Conclusion: In general, pregnant women had good oral health, which improved after participating in the program, and good oral quality of life, with a negative influence due to caries lesions and plaque. In all study groups concentration values dropped to baseline 15 and 30 days after the application of fluoride.
  • Publication
    Open Access
    Diseño y evaluación de una cartilla de salud oral durante el embarazo
    (Elsevier, 2025) Marina Alcaraz-Orcajada; Elena Sanchez Guerrero; Serna Muñoz, Clara; Pérez Silva, Amparo; Ortiz Ruiz, Antonio José; Martínez Beneyto, Yolanda; Dermatologia, Estomatología y Radiología y Medicina Física; Medicina
    Objetivo: El principal objetivo de este estudio fue dise˜nar y evaluar una cartilla bucodental para gestantes con el fin de mejorar la prevención y el seguimiento de enfermedades bucodentales en este grupo. Los objetivos específicos incluyeron dos fases complementarias: 1) la validación de la cartilla por profesionales del ámbito odontológico, y 2) su implementación en una población de mujeres gestantes para valorar su utilidad práctica en la promoción de la salud oral. Dise˜no: Estudio descriptivo observacional transversal desarrollado en dos fases consecutivas. En la primera fase se elaboró y validó la cartilla mediante la evaluación de 40 profesionales del ámbito odontológico, y en la segunda se implementó en una muestra de 241 gestantes. Emplazamiento: Clínica Odontológica Universitaria del Hospital Morales Meseguer (Región de Murcia), dentro del programa de salud bucodental de la Universidad de Murcia dirigido a gestantes. Participantes: Un total de 241 gestantes entre las 10 y 31 semanas de embarazo, seleccionadas mediante matronas de centros de atención primaria y difusión en redes sociales. Método: Se emplearon cuestionarios, historias clínicas y exploraciones bucodentales para recopilar los datos. Se analizaron hábitos de salud oral, índices de caries, placa y sangrado gingival, y variables sociodemográficas. Los resultados de la validación profesional se utilizaron para perfeccionar el dise˜no final de la cartilla.Resultados: La cartilla fue bien aceptada por los profesionales, quienes destacaron su utilidady claridad. En la población de gestantes se observó una alta prevalencia de caries (CAOD: 7,34)y placa (30,15%), lo que resalta la necesidad de programas preventivos durante el embarazo.Conclusiones: La cartilla constituye un recurso valioso para el seguimiento y la promoción de lasalud bucodental en mujeres embarazadas. Su aplicación en atención primaria facilita la inte-gración de la salud oral en el control prenatal y fomenta la educación sanitaria y la prevenciónde enfermedades