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Madrid Sánchez, Josefa

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Madrid Sánchez, Josefa
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Universidad de Murcia. Departamento de Producción Animal
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    Effects of Commercial Antioxidants in Feed on Growth Performance and Oxidative Stress Status ofWeaned Piglets
    (MDPI AG, 2021-01-21) Orengo Femenia, Juan; Hernández Ruipérez, Fuensanta; Martínez Miró, Silvia; Sánchez Parra, Cristian Jesús; Peres Rubio, Camila; Madrid Sánchez, Josefa; Producción Animal
    This work aimed to evaluate the effect of adding two different commercial antioxidants (AOX) products to pre-starter and starter diets using low vitamin E (Vit E as DL- -tocopheryl acetate) levels on the growth performance and oxidative stress of piglets for the first six weeks post-weaning (PW). They were sorted by initial body weight (BW: 6.175 0.931 kg) and randomly allotted to four dietary treatments (with six replicates per treatment): a positive control (PC) and a negative control (NC) diet, with normal and low dose of vitamin E (80 and 15 mg kg􀀀1, respectively), both without AOX; the other two experimental diets with a low dose of vitamin E (LVE) plus LOXIDAN VD100 (LVE + AOX1) or LOXIDAN E Ros (LVE + AOX2). Growth data were recorded, and blood samples were taken, at the beginning (day 0) and at the end of each feeding period: pre-starter and starter (at days 14 and 42, respectively). No differences among dietary treatments were found with respect to growth performance in the pre-starter period (p 0.05). However, at the end of the starter period, a lower BW was found in piglets fed the NC diet compared to the other dietary treatments. Differences in daily gain and feed conversion ratio were also found either for the starter period or when the whole period was considered (p < 0.05), whereby piglets fed PC or LVE diets supplemented with AOX showed better growth performance compared to piglets fed the NC diet. Regarding Vit E ( -tocopherol) serum levels, there were no differences among treatments at day 0; but the serum values of this vitamin decreased in LVE diets at 14 and 42 days, but not in the PC. On day 42, the highest levels of -tocopherol in liver were also found in piglets fed PC (p < 0.05). Nevertheless, in general, from a metabolic point of view and after checking the serum biochemical profile of piglets, there were no differences in other oxidative stress markers (p 0.05). The results showed that the AOX products used were able to compensate for the lower Vit E supply with respect to growth performance in the starter phase. The use of AOXs or usual levels of Vit E in feed constitutes a key factor in achieving optimal growth performance of piglets in the PW period.
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    Open Access
    Physicochemical characteristics of fibre sources and their effects on in vitro Escherichia coli infection in IPEC-J2 cells
    (Taylor & Francis, 2025-05-09) Ruiz, Eduardo; Martínez Miró, Silvia; Hernández Ruipérez, Fuensanta; Ayala García, Lucía; Madrid Sánchez, Josefa; Ortega Hernández, Nieves; Producción Animal
    Feed-related strategies, such as adding fibre to weaned-piglets’ feed, have been suggested to reduce the impact of Escherichia coli (E. coli) in swine production. We performed physicochemical analysis of fibre sources (wheat bran, commercial insoluble fibre (commercial IF), almond shell, beet pulp, dry olive cake, and oat hull) and examined the effects of their soluble fractions on fimbriated (F4) and non-fimbriated (NF) E. coli. Intestinal porcine epithelial (IPEC-J2) cells were used as an in vitro model. Almond shell and commercial IF contained more insoluble and total dietary fibre (p < 0.001), while wheat bran contained the least total dietary fibre (p < 0.001). Beet pulp contained the most soluble fibre and had the highest water holding capacity (p < 0.001). Commercial IF had the highest swelling capacity (p < 0.001) and dry olive cake the highest cold solubility (p < 0.001). Based on adherence assays, wheat bran, oat hull, almond shell, and dry olive cake significantly prevented E. coli F4 from adhering to IPEC-J2 cells, whereas for NF E. coli, only dry olive cake reduced bacterial infection (p < 0.05). Dry olive cake was the most effective in reducing expression of proinflammatory cytokines, predominantly interleukin 8 (IL-8) and tumour necrosis factor-α (TNF-α) (p < 0.001). In conclusion, aqueous extracts of wheat bran, oat hull, almond shell, and dry olive cake contain compounds that interfere with E. coli F4 adherence to IPEC-J2 cells.
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    Open Access
    Effect of sow body weight at first service on body status and performance during first parity and lifetime
    (MDPI, 2022-12-02) Carrión López, María José; Orengo Femenia, Juan; Madrid Sánchez, Josefa; Vargas, Antonio; Martínez Miró, Silvia; Producción Animal
    In this retrospective study, we aimed to assess the effect of body weight (BW) at first service on body status development and sow performance during first parity and lifetime efficiency. A total of 360 DanBred gilts were used, which we categorized into three groups according to their BW: (1) Small sows (<135 kg BW; n = 108); (2) Medium sows (135–150 kg BW; n = 155); (3) Large sows (>150 kg BW; n = 63). We studied the gilts from first insemination to first weaning, and then monitored until culling. Sow body status, litter size and weight, farrowing rate, weaning-to-conception interval, lifetime performance, and hormones linked to metabolism were recorded. Sows in the Small group had the lowest body weight, backfat thickness, and loin depth during gestation. Moreover, they had the smallest number of total piglets born and longest weaning-to-conception interval at first parity. The Small sows also had, on average, one less piglet born during their productive life, and they tended to have a higher culling rate over three parities. For immunoglobulins, insulin, cortisol, and IGF-I levels, no differences were found. In conclusion, achieving optimal body weight at first service is essential for maximizing the sow’s lifetime performance.
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