Histology and histopathology Vol. 5, nº 3 (1990)
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- PublicationOpen AccessThe autonomic innervation of the liver and gallbladder of Podarcis hispanica(Murcia : F. Hernández, 1990) Azanza, M.J.; Aisa, J.; Junquera, C.The innervation of the liver and gallbladder of the lizard Podarcis hispanica has been studied by the following methods: a) demonstration of cholinesterase activity; b) FIF method for catecholamines; and c) immunohistochemistry for VIP. The hepatic parenchyma of the reptile's liver show hzpatocytes arranged in regular rows of hepatic cords, the portal triad being typical of higher vertebrates (birds and mammals). Nerve fibers are found in the scarce connective tissue distributed among the hepatocytes. The innervation is restricted to the big branches of blood vessels and biliary ducts. It is represented by cholinergic, noradrenergic and VIPergic fibers. The gallbladder shows a well developed cholinergic plexus with pyramidal cells in the interconnection points of the fiber network. The noradrenergic and VIPergic plexuses are also more widely distributed in the gallbladder thali in the liver.
- PublicationOpen AccessHigh resolution SEM analysis of acellular glomerular basement membrane following pepsin digestion, intrinsic fibrillar structures(Murcia : F. Hernández, 1990) Berger, Walter J.; Carlson, E. C.Microdissection of acellular rat renal cortex with pepsin was carried out to investigate the morphological substructure of glomerular basement membrane (GBM) by high resolution SEM. Renal cortical blocks (< 5 mm ) from adult male Sprague Dawley rats were rendered acellular by sequential detergent extraction and digested up to 184 hrs with 5 mglml pepsin (185 U/ mg) in 0.5 M acetic acid (pH 2) at 10-15°C. Samples were conventionally prepared for SEM, and observed at original magnifications of 500-100,000 diameters. At low magnifications (500-5,00Ox), acellular GBM surfaces appeared smooth at al1 digestion times. At higher magnifications (50,000-100,00Ox), control GBM surfaces were finely granular. Granule diameter ranged from 20-80 nm, with most between 30-40 nm. Pepsin digestion did not affect average granule size. Beginning at 44 hrs of digestion, intrinsic fibrillar structures comprised of linear arrays of 20-40 nm granules were observed onlin GBM surfaces. At later incubation times, this component of GBM became more extensive. At 160 hrs, the fibrillar arrays frequently bifurcated and showed distinctive «forked» termini, some of which comprised two sides of a triangle (120-150 nm on a side). Fork «handles» (310-350 nm in length) radiated from each angle of the triangle. These sometimes terminated in large granules (approximately 100 nm in diameter), two of which appeared to connect fibrillar arrays end-toend. Together with other arrays, the interconnected triangles appeared to comprise a three-dimensional meshwork extending into the GBM and possibly providing support for, its granular components.
- PublicationOpen AccessVasopressinergic neurons in periependymal and periventricular areas of the rostral third ventricle(Murcia : F. Hernández, 1990) Roy Choudhury, S.; Ray, P.K.On the lateral wall of the rostral third ventricle, an area separates the ependyma from the neurosecretory PVN neurons. Since VP from the latter discharges into the vasculature, the above area may be regarded as constituting an interface between the ventncular and vascular compartments of the CNS. As VP release into the two compartments is integrated, the interface region has been explored for possible existence of a neural infrastructure that would allow such an integration. Immunohistochemical staining for VP following colchicine treatment reveals the presence of an elaborate vasopressinergic network in the interface region that is divisible into a media1 periependymal and a lateral periventricular area. A closer examination indicates that the ependymal, periependymal, periventricular and PVN areas (in that order medio-laterally) are al1 interconnected through this network. The media1 area appears to be receptive in nature, while the connectivity of the lateral area points to an effector function. All in all, such a neural network would provide a sound morphological basis for integration of neuroendocrine mechanisms modulating VP release into the ventricular and vascular compartments of the CNS.
- PublicationOpen AccessHuman pulmonary dirofilariasis, report of a new European case(Murcia : F. Hernández, 1990) Fabbretti, G.; Fedeli, F.; Alessi, A.; Boaron, M.; Salpietro, V.; Brisigotti, M.We report a new European case of pulmonary dirofilariasis occurring in an Italian patient. The paper emphasizes the peculiar pathological features of Pulmonary Dirofilariasis, that, on clinical and radiological grounds, closely irriitates primary or secondary neoplasms. The disease characteristically presents itrelf as a solitary subpleural coin-like lesion, histologically corresponding to a well demarcatec,, roughly spherical infarct, centered by a medium-sized thrombosed artery whose lumen contains the parasite, i.e. a Dirofilaria nematode.
- PublicationOpen AccessUltrastructural characteristics of anterior gut innervation of Gallus gallus(Murcia : F. Hernández, 1990) Aisa, J.; Parra, P.; Azanza, M.J.The enteric nervous system of the bird's anterior gut is very well developed. Myelin fibres are seen accompanying the nervous trunks up to the mucous layer. Glial cells duplicate the number of neurons in the myenteric plexuses. Their number decreases at the submucous plexuses, but it is always higher than the neurons. Isolated neurons are widely spread in the circular muscle coat accompanying the nervous trunks which can be inter and intrafascicularly located. Direct synaptic contacts with the soma neurona1 membranes are very often seen. We have never observed synaptic specializations. The most prominent varicosities either in the peripheric nervous trunk axons or directly laying on the soma membranes are those containing peptidergic or mixed vesicles of cholinergic and peptidergic types. The neurons show big nuclei of different size and shape. Neighbouring smooth muscle cells show abundant caveolae near the nervous elements. Although we have not observed close contacts with glands, thin axon bundles spread near the glandular cells of the mucous layer .