Histology and histopathology Vol.25,nº10 (2010)
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- PublicationOpen AccessUltrastructural morphology of equine adipose-derived mesenchymal stem cells(Murcia : F. Hernández, 2010) Pascucci, Luisa; Mercati, Francesca; Marini, Carla; Ceccarelli, Piero; Dall’Aglio, C.; Pedini, Vera; Gargiulo, Anna MaríaMesenchymal stem cells are a virtually ubiquitous population of adult stem cells, able to differentiate into various tissue lineages. As they are multipotent and easy to grow in culture, they are at present considered very attractive candidates for tissue repair and gene therapy. With the exception of a few reports, mesenchymal stem cell morphology has been widely disregarded in the past years. In this paper we discuss the establishment of mesenchymal stem cell cultures from equine adipose tissue and describe their fine structure by transmission electron microscopy. The cultured cells revealed a fibroblastoid appearance and were characterized by an eccentric nucleus with multiple nucleoli, dense cytoplasm rich in ribosomes, a rough endoplasmic reticulum with dilated cisternae, elongated mitochondria and heterogeneous vacuolar inclusions. In addition, they were often interconnected by adhesion structures located on the cell body and on cytoplasmic processes contacting other cells. The features observed are evocative of an undifferentiated cellular phenotype and of an intense synthetic and metabolic activity.
- PublicationOpen AccessComparison of ultrastructure and lectin histochemistry on the anterior medial gland of nasal septum in rat and gerbil(Murcia : F. Hernández, 2010) Chang, Chi-Fen; Chau, Yat-Pang; Lu, Kuo-ShyanThe anterior medial gland (AMG), located in the submucosa of rodent nasal septum, is suggested to provide fluid for humidification of inspired air. Tremendous variation of the environmental air humidity, on which rats and gerbils depend to live, leads us to expect a multiplicity on ultrastructure and various subcellular glycoconjugate distribution within the AMG acinar cells between these two species. Electron microscopy revealed that: (1) The nucleus of AMG acinar cells in rat was irregular-shaped, but that in gerbil was round or elliptical; (2) Secretory granules in rat AMG acinar cells contained homogenous content with various electron density. However, two types of secretory granules in gerbil AMG acinar cells were found: one with lamellated-structure and high electron density, while the others had particulate materials; (3) Myoepithelial cells were present in the acinus of medial and posterior regions in rat AMG, but absent in gerbil; and (4) Nerve terminals were present only in the medial and posterior rat AMG, but in all three regions of the gerbil AMG. Lectin histochemistry demonstrated that: (1) Rat and gerbil AMG acinar cells expressed strong affinity toward Con A and WGA, but neither showed any reactivity toward UEA and PNA; and (2) Varying degrees of reactivity toward different lectins, including DBA, PNA, SBA and EBL, were recognized in rat and gerbil AMG acinar cells. We confirm the species variation on the ultrastructure and lectin histochemistry of AMG in rats and gerbils, and speculate that these variations may be due to the different living environment.
- PublicationOpen AccessThe cellular expression of GABAA receptor a1 subunit during spermatogenesis in the mouse testis(Murcia : F. Hernández, 2010) Kanbara, Kiyoto; Okamoto, Keiko; Nomura, Sakashi; Kaneko, Takeshi; Watanabe, Masahito; Otsuki, YoshinoriGABAA receptors are pentamers in structure and are mainly composed of α, ß and γ subunits. These receptors are known to function as chloride channels. We observed α5, ß1 and γ3 subunit immunoreactivity in the mouse testes, specifically in the cytoplasm surrounding the nucleus in the spermatocytes and spermatids. In the current study, α1 subunit immunoreactivity was located in the nucleus of spermatogonia, spermatocytes and round spermatids. Immunoelectron microscopy revealed that the α1 subunit was localized within the nucleus of pachytene and diplotene spermatocytes in the area of condensed chromatin rather than extended chromatin. Protein sequence analysis revealed that the α1 subunit included DM DNA binding domains that were related to transcription factors involved in testicular differentiation in adult mice. These findings suggest that the α1 subunit may undertake a gene transcription function during the maturation of germ cells. α1 immunoreactivity was also detected within the mitochondria of spermatocytes and in the acrosome of round and elongated spermatids. Although the precise physiological role of the GABAA receptor α1 subunit in mitochondria remains unknown, we hypothesize that its function in the acrosome may be related to the acrosome reaction during fertilization or during spermatogenesis.
- PublicationOpen AccessLung carcinoma with rhabdoid component. A series of seven cases associated with uncommon types of non-small cell lung carcinomas and alveolar entrapment(Murcia : F. Hernández, 2010) Izquierdo Garcia, Francisco M.; Moreno Mata, Nicolás; Herranz Aladro, María Luisa; Cañizares, Miguel Ángel; Álvarez-Fernández, EmilioRhabdoid tumor, included in the WHO classification among large cell carcinomas of the lung, is an uncommon type of lung carcinoma with poor prognosis. We report a series of 7 cases of lung carcinomas with rhabdoid component in 10% and 80% of the tumor. The associated tumor was adenocarcinoma in 3 cases - one of them with focal micropapillary pattern - large cell carcinoma in 2 cases, squamous cell carcinoma in 1 case and pleomorphic carcinoma in 1 case. Two adenocarcinomas showed a focal spindle cell component. Micropapillary and pleomorphic types had not been reported before as a component associated with rhabdoid carcinomas. All cases were positive for vimentin, and AE1/AE3 cytokeratin and 5 cases for cytokeratin 7. All cases were negative for muscle and endothelial markers and for chromogranin A. Synaptophysin was focally positive only in one case. Alveolar trapping inside the tumor was present in 3 cases - a phenomenon not well studied in lung carcinomas and also not reported in tumors with rhabdoid component. Five patients died because of the tumor within 2 to 31 months after diagnosis, one of myocardial infarction and only one is alive and disease free 123 months after the diagnosis. In summary, we describe 7 new cases of this uncommon lung tumor with aggressive clinical course, associated with infrequent histological types in nonrhabdoid component and with alveolar trapping, a nondescribed finding.
- PublicationOpen AccessIntermediate conductance Ca2+ activated K+ channels are expressed and functional in breast adenocarcinomas: correlation with tumour grade and metastasis status(Murcia : F. Hernández, 2010) Haren, Nathalie; Khorsi, Hafida; Faouzi, Malika; Ahidouch, Ahmed; Sevestre, Henri; Ouadid-Ahidouch, HalimaK+ channels are key molecules in the progression of several cancer types and are considered to be potential targets for cancer therapy. In this study, we investigated the intermediateconductance Ca2+-activated K+ channels (hKCa3.1) expression in both breast carcinoma (BC) specimens and human breast cancer epithelial primary cell cultures (hBCE) using immuno-histochemistry (60 samples), quantitative Real-Time RT-PCR (30 samples) and Western blot assay (30 samples). We also looked at whether or not the expression of these channels is correlated with breast carcinomas grade tumours and metastasis status. Furthermore, we characterized the hKCa3.1 channel activity in hBCE cells by using the Whole Cell Patch Clamp Technique. We found that hKCa3.1 transcripts and proteins were expressed in both BC samples and hBCE cells. Clinicopathologic evaluation indicated a significant correlation between hKCa3.1-expression and tumour grade. hKCa3.1 mRNA and protein were more highly expressed in grade III tumours than in both grades I and II. However, the hKCa3.1 expression-increase according to grade was only observed in tumours with negative metastasis status. Moreover, the hKCa3.1 channels expressed in hBCE cells are functional. This was attested by patch-clamp recordings showing typical hKCa3.1-mediated currents in these cells. In conclusion, these data suggest that hKCa3.1 might contribute to breast tumour-progression and can serve as a useful prognostic marker for breast cancer
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