Histology and histopathology Vol.34, nº2 (2019)
Ir a Estadísticas
Permanent URI for this collection
Browse
Recent Submissions
- PublicationOpen AccessCO2 laser therapy accelerates the healing of ulcers in the oral mucosa by inducing the expressions of heat shock protein-70 and tenascin C(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Gang, Luo; Oka, Kyoko; Ohki, Shirabe; Rikitake, Mihoko; Itaya, Satoshi; Tamura, Shougo; Toda Nakamura, Masako; Ogata, Kayoko; Kira Tatsuok, Michiko a; Ozaki, MasaoThe treatment of ulceration or stomatitis with laser therapy is known to accelerate healing and relieve pain, but the underlying biological mechanism is not fully understood. The present study used a mouse model of ulceration to investigate the molecular mechanisms by which CO2 laser therapy accelerated the wound healing process. An ulcer was experimentally created in the palatal mucosa of the mouse and irradiated with light from a CO2 laser. Compared with controls (no irradiation), laser irradiation induced the proliferation of epithelial cells and faster re-epithelialization of the wound area. Immunohistochemistry experiments showed that heat shock protein-70 (HSP70) was expressed mainly in the epithelium of normal palatal tissue, whereas there was little tenascin C (TnC) expression in the epithelium and mesenchyme under normal conditions. Laser irradiation induced HSP70 mRNA and protein expression in the lamina propria as well as TnC expression in the mesenchyme underlying the renewing epithelium. Epithelial cells and fibroblasts were exposed to heated culture medium or laser irradiation to establish whether hyperthermia mimicked the effect of laser irradiation. Culture of fibroblasts in heated medium increased the expressions of both TnC and TGF-β1, whereas laser irradiation induced only TnC expression. The present study indicates that CO2 laser irradiation exerts a photobiogenic effect to up-regulate TnC expression without inducing TGF-β1 expression. We suggest that CO2 laser therapy has an advantage over thermal stimulation.
- PublicationOpen AccessExpression of glutamine metabolism-related proteins in Hürthle cell neoplasm of thyroid: Comparison with follicular neoplasm(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Cha, Yoon Jin; Jang, Haerin; Koo, Ja SeungPurpose. We evaluated the expression of glutaminolysis-related proteins in Hurthle cell neoplasms (HCN) and follicular neoplasms (FN) of the thyroid, and investigated its clinical implication. Methods. Tissue microarrays were constructed from 264 FNs (112 follicular carcinomas [FCs] and 152 follicular adenomas [FAs]) and 108 HCNs (27 Hurthle cell carcinomas [HCCs] and 81 Hurthle cell adenomas [HCAs]. The immunohistochemical staining result of 3 glutaminolysis-related proteins (Glutaminase 1 [GLS1], glutaminate dehydrogenase [GDH] and alanine- serine, cysteine-preferring transporter 2 [ASCT2]) was analyzed. Results. GLS1 and GDH showed significantly higher expression rates in HCN compared to FN (P<0.001). More HCN cases showed co-positivity of multiple glutaminolysis-related proteins than those of FN cases (P<0.001). In silico analysis, both GLUD1 and GLUD2 showed higher expression rate in HCA compared to FA (P=0.027 and P=0.018, respectively). SLC1A5 expression was highest in HCA, followed by FC and FA (HCA vs FC, P=0.023; FC vs FA, P=0.002). Conclusion. FN and HCN exhibit a different expression pattern for glutaminolysis-related proteins, and GLS1 and GDH have higher expression rates in HCN and FN.
- PublicationOpen AccessInterleukin-6: a molecule with complex biological impact in cancer(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Lacina, Lukáš; Brábek, Jan; Král, Vladimír; Kodet, Ondřej; Smetana Jr, KarelInterleukin-6 is a multifaceted cytokine, usually reported as a pro-inflammatory molecule. However, certain anti-inflammatory activities were also attributed to IL-6. The levels of IL-6 in serum as well as in other biological fluids are elevated in an agedependent manner. Notably, it is consistently reported also as a key feature of the senescence-associated secretory phenotype. In the elderly, this cytokine participates in the initiation of catabolism resulting in, e.g. sarcopenia. It can cross the blood-brain barrier, and so it is in causal association with, e.g. depression, bipolar disorder, schizophrenia, and anorexia. In the cancer patient, IL-6 is produced by cancer and stromal cells and actively participates in their crosstalk. IL-6 supports tumour growth and metastasising in terminal patients, and it significantly engages in cancer cachexia (including anorexia) and depression associated with malignancy. The pharmacological treatment impairing IL-6 signalling represents a potential mechanism of antitumour therapy targeting cancer growth, metastatic spread, metabolic deterioration and terminal cachexia in patients.
- PublicationOpen AccessMature congenital intraneural teratoma in cerebellum of pig(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Soto Domíngue, Adolfo; Rodríguez Rocha, Humberto; García García, Aracely; Saucedo Cárdenas, Odila; Rodríguez Tovar, Luis E.; Castillo Velázquez, UzielThe biological behavior of teratomas depends on several interdependent clinical and epidemiological variables such as age at diagnosis, sex, tumor microenvironment, and tumor morphology, among others. All these variables are correlated to different cytogenetic and molecular aberrations (Harms et al., 2006). There are null reports of teratomas in pigs. The aim of this study was to characterize the tissues present in a mature congenital intraneural teratoma in the cerebellum area of a Landrace female pig of 6-7 weeks old. In this study, tissue control samples were used to validate each staining method. Sections from the teratoma showed normal histology of the cerebellum, including rounded Purkinje neurons with abundant cytoplasm, euchromatic nuclei, and prominent nucleoli; glial cells with a scarce amount of cytoplasm and small and highly basophile-nuclei (compact chromatin) and axonal tracts (white matter). Interestingly, we also observed areas with tissues different from the nervous tissue, including bundles of well-defined skeletal muscle fibers with a striated pattern and peripheral nuclei; hyaline cartilage plaques, with prominent presence of chondrocytes in their lagoons forming isogenous groups surrounded by a territorial and interterritorial matrix; trabeculated bone tissue; and adipocytes, which are ringshaped cells with peripheral flattened nuclei, as a result of the presence of a central large lipid droplet. To our knowledge, this study is the first to describe a congenital intraneural mature teratoma in the cerebellum of a pig.
- PublicationOpen AccessExpression of WIF-1 in inflammatory bowel disease(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Terry, Robert; Chintanaboina, JayaKrishna; Patel, Deep; Lippert, Brittany; Haner, Matthew; Price, Kimberly; Tracy, Amanda; Lalos, Alexander; Wakeley, Michelle; Gutierrez, Linda S.The WNT/β-catenin cellular network has been extensively studied in numerous diseases including inflammatory bowel disease (IBD). IBD is a condition that increases the risk of developing colorectal cancer. WIF-1 is an inhibitory protein that acts by blocking the interactions of WNT with its receptor complex, thus leading to downregulation of end products of this pathway. While WIF-1 has been characterized in several cancers, its relationship with IBD has yet to be elucidated. In this study, the expression of WIF-1 in patients with IBD was analyzed in order to provide insights into the pathophysiology and rationale for alternative therapies. Biopsies of both normal and inflamed colonic mucosa from patients with Crohn’s disease or ulcerative colitis were histologically examined for the degree of morphologic changes, immune cell infiltration and presence of WIF-1 through immunohistochemistry. No differences were observed in WIF-1 expression linked to a particular condition, but WIF-1 stain was significantly enhanced in the crypts and lamina propria as inflammation increased in biopsies from patients with both, ulcerative colitis and Crohn’s disease. These findings could give guidance to new therapeutic applications of the WNT/β-catenin system and WIF-1 in IBD.