Browsing by Subject "TNF-α"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Publication
    Open Access
    A systematic review of the cardiometabolic benefits of plant products containing mixed phenolics and polyphenols in postmenopausal women: insufficient evidence for recommendations to this specific population
    (MDPI, 2021-11-27) Sánchez-Martínez, Lorena; Periago, María Jesús; García-Alonso, Javier; García-Conesa, María Teresa; González-Barrio, Rocío; Tecnología de Alimentos, Nutrición y Bromatología
    Menopause is a critical stage in a woman’s life in which cardiometabolic alterations appear, such as insulin resistance or a predisposition to visceral fat deposits, leading to an increased risk of cardiometabolic diseases (R-CMBs). New strategies to reduce the R-CMBs in postmenopausal women using natural compounds without adverse effects are desirable. In this sense, plant-based diets rich in fruits and vegetables could play a fundamental role due to the high content of bioactive compounds found in these diets, such as (poly)phenols, known for their antioxidant, anti-inflammatory and vasodilator properties. The aim of this research was to carry out a dietary trial to evaluate the effect of the daily intake of different (poly)phenol-rich foods (PP-rich foods) for 2 months on the modulation of the main cardiometabolic risk biomarkers of postmenopausal women. The results showed a slight improvement in blood pressure (BP), lipid profile and oxidative stress, endothelial function and inflammatory biomarkers. These findings suggest that daily consumption of PP-rich foods alleviated the R-CMBs of postmenopausal women by reducing the oxidative stress and, thus, the risk of cardiovascular events; however, the magnitude of the cardioprotective effect of (poly)phenols depends on inter-individual variability.
  • Thumbnail Image
    Publication
    Open Access
    Optimizing the preparation of silk fibroin nanoparticles and their loading with polyphenols: towards a more efficient anti-inflammatory effect on macrophages
    (MDPI, 2023-01-12) Ruiz Alcaraz, Antonio José; Núñez-Sánchez, María Ángeles; Asensio Ruiz, María Alejandra; Martínez-Sánchez, María Antonia; Oliva-Bolarín, Alba; Martínez Martínez, Teresa; Pérez Cuadrado, José Julián; Ramos-Molina, Bruno; Lozano-Pérez, Antonio Abel; Bioquímica y Biología Molecular B e Inmunología; Facultad de Biología
    Silk fibroin nanoparticles (SFN) have become a promising tool in drug delivery systems due to their physicochemical characteristics. SFN have shown their outstanding properties as an active vehicle for polyphenols, enhancing their antioxidant and anti-inflammatory effects on macrophages; therefore, it becomes necessary to have an easy, reproducible and scalable production method. In order to improve the production of nanoparticles, we performed direct precipitation of non-dialyzed silk fibroin solutions and evaluated the reproducibility of the method using dynamic light scattering. We also studied the loading efficiency of three different natural polyphenols using propylene glycol as a solvent. The loaded nanoparticles were fully characterized and used to treat human macrophage cells to assess the anti-inflammatory activity of these nanoparticles. The measured hydrodynamic characteristics of the SFN and the overall yield of the process showed that the new preparation method is highly reproducible and repeatable. Thus, we not only present a new scalable method to prepare silk nanoparticles but also how to improve the loading of natural polyphenolic compounds to the SFN, as well as the important anti-inflammatory effects of these loaded nanoparticles in a cell model of human macrophage cells.
  • Thumbnail Image
    Publication
    Open Access
    Yolkin tempers inflammatory mediator release and liver pathology in experimental endotoxemia in mice
    (2026) Jolanta Artym; Maja Kocięba; Ewa Zaczyńska; Kaleta-Kuratewicz; Jan P. Madej; Piotr Kuropka; Aleksandra Zambrowicz; Łukasz Bobak; Michał Zimecki; Biología Celular e Histología; Universidad de Murcia, Departamento de Biologia Celular e Histiologia
    Yolkin is an egg yolk-derived protein with immunoregulatory properties. In this work, yolkin was evaluated as a protective agent in endotoxemic BALB/c mice. The mice were pretreated with yolkin either orally in drinking water or intraperitoneally (i.p.) before i.p. injection of E. coli lipopolysaccharide (LPS). Circulating blood leukocyte number, blood cell composition, serum levels of tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and haptoglobin, as well as histological changes in the spleen and the liver, were examined. Yolkin differentially regulated the values of these parameters, depending on the administration protocol; however, the serum levels of TNF-α and IL-6 were generally decreased, and the level of haptoglobin, an acute-phase protein, was elevated. The pretreatment of mice with yolkin led to improved histological architecture in the investigated organs of endotoxemic mice, particularly in the liver, where yolkin diminished an increased level of vascular permeability and reversed a decreased number of Kupffer cells. These changes were independent of the route of yolkin administration. In conclusion, yolkin proved effective in the amelioration of pathogenic consequences of LPS administration and may be considered a potential protective measure for patients at risk of endotoxemia. Histol Histopathol