Browsing by Subject "Stabilization"

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    Highly selective biocatalytic synthesis of monoacylglycerides in sponge-like ionic liquids
    (RSC, 2017) Gómez, Celia; Sánchez-Gómez, Gregorio; García-Verdugo, Eduardo; Luis, Santiago V.; Lozano Rodríguez, Pedro; Nieto Cerón, Susana; Bioquímica y Biología Molecular B e Inmunología
    The biocatalytic synthesis of monoacylglycerides (MAGs) was carried out by the direct esterification of fatty acids (i.e. oleic, palmitic, myristic and lauric acids, respectively) with glycerol in different ionic liquids (ILs) based on cations with long alkyl side-chains (i.e. 1-hexadecyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide [C16mim][NTf2], 1-dodecyl-3-methylimidazolium tetrafluoroborate [C12mim][BF4]). Although all ILs have been shown as suitable reaction media for Novozym 435-catalyzed esterification of glycerol with free fatty acid, a highly selective selectivity of MAGs was only observed for the [C12mim][BF4] case (i.e up to 99% selectivity and yield for the monolaurin case). Furthermore, as these ILs are temperature switchable ionic liquid/solid phases that behave as sponge-like system, a straightforward protocol for IL-free MSGs recovery, based on iterative centrifugations at controlled temperature, has been developed.
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    Nanoparticles of betalamic acid derivatives with cyclodextrins. Physicochemistry, production characterization and stability
    (Elsevier, 2020-07-14) Guerrero Rubio, Alejandra; Matencio Durán, Adrián; Gandía Herrero, Fernando; López Nicolás, José Manuel; García Carmona, Francisco; Bioquímica y Biología Molecular A
    The use of betalains as bioactive compounds has been widely investigated. However, their use as a functional food requires a more exhaustive study because of their photosensitivity and poor stability. For this reason, this works studies the stabilization of two highly bioactive betalain derivatives, phenylethylamine-betaxanthin (Ph- Bx) and indoline-betacyanin (In-Bc) by nanoencapsulation in natural a modified cyclodextrins (CDs). Methyl- β-CD (Mβ-CD) showed the best results for Ph-Bx while Hydroxypropyl-β-CD (HPβ-CD) was better in the case of In- Bc. In addition, the effect of pH and temperature on encapsulation was studied finding that the encapsulation constant (KF) value increased as the pH decreased in the case of Ph-Bx but not in the case of In-Bc. The KF decreased as the temperatures increased. The thermodynamic parameters, ΔH◦, ΔS◦ and ΔG◦, were also calculated. Molecular docking provided further insight into how the different interactions influenced the encapsulation constant. The results show that the presence of CDs during the biotechnological synthesis of Ph-Bx and In- Bc increased production by 51 and 26%, respectively, and also increased the stability of both derivatives. This study represents a first step towards increasing the production of this type of compound for introduction into functional foods.