Browsing by Subject "Resveratrol"
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- PublicationOpen AccessAntioxidant activity of resveratrol compared with common food additives(Elsevier, International Association for Food Protection, 2001-03) Murcia Tomás, María Antonia; Martínez Tomé, Magdalena; Tecnología de Alimentos, Nutrición y BromatologíaResveratrol is a phenolic compound of the stilbene family present in wines and various parts of the grape, including the skin. In this study, the antiox.idant and prooxidant activities of resveratrol were compared with other antiox.idants (butylated hydroxytoluene [BHT]. burylated hydroxyacetone (BHA). phenol, propyl gallate (PG), sodium tripolyphosphate [TPP], atocopherol, and vanillin) widely used in foods. Toe ability of these compounds to in!úbit lipid perox.idation was as follows: BHA > resveratrol > PG > tripolyphosphate > vanillin > phenol > BHT > a-tocopherol, the first three inlúbiting the perox.idation in a concentration-dependent manner. Toe order of OH· scavenger activity of the tested compounds was BHA > TPP > BHT Resveratrol and vanillin produced between 10 to 7% and 16 to 10% inhibition of the deoxyribose attack, respectively, but they do not scavenge OH·. Neither the resveratrol analyzed nor PG or the rest of compounds reacted with H and must be considered inefficient in catalyzing any subsequent ox.idation. The ability to scavenge HOCI was, in decreasing order, PG > resveratrol > cc.-tocopherol > phenol. The other compounds did not scavenge HOCI.
- PublicationOpen AccessAntioxidant properties of mediterranean spices compared with common food additives.(Elsevier, International Association for Food Protection, 2001-09) Martínez Tomé, Magdalena; Jiménez Monreal, Antonia M.; Ruggieri, Silverio; Frega, Natale; Strabbioli, Rosanna; Murcia, M. Antonia; Tecnología de Alimentos, Nutrición y BromatologíaIn 1his study, thc antioxid:mt propcrtics of Mcditcrranean food spiccs (annano, cumin. orcgano, swc.c.: and hot paprik.:i. rosemJJy. aqd saffron) at $% concenttation and of common food addi1ivt$ (butylatoo hydmxyaoisole (BHAI, butylntcd hy· c::iroxytolt1cne [OHT]. and propyl gallate) at 100 µ.g/g are compared. Thc abili1y of thesc compoonds to inhibit lipid peroxid:uion was in docrcasing order. roscmary > onJ:g.:ino >. propyl gallate > :rnmmo > BHA > swcct paprika > cumin > hot paprikaprolec:tivc actlon. Toe rest ofthc spices al.so protecc dooxyr!bose bctter than 1he BHA and i3HT us.cd·in \lle :issay. Finally. the resulLS obtaincd· in thc assay point to rhe pCO,Oxid:ml effcct of propyl gall.ate. lfydrogen i>Croxidc sc:avcnging activity is mea.sured i,y using peloxicbsc·bascd ass3y systems. In aqucous tnedium, lhc s::,icc extracts shOw lower antioxidain activity tha.n propyl ga.llate. thC d.:creasing ordcr bcing cumin > orcgano > anl'.l-3no > cosem.uy > hOl paprilfa > swcet paprik.a. 8HA and SHT did not scavenge H20:!.. Spio::s,ace :a.ble to scavcngc HOCL :tnd protecl arantiproteinase. Toe results indic:i.te th.:t rosemary :and o,egano 1rc more effccti\'t: HOCJ scavcng.crs tMn the othc.r subst:anccs analyzcd. wich. in decrc:.\Sing o
- PublicationOpen AccessBiotechnological approach to increase oxyresveratrol production in mulberry in vitro plants under elicitation(MDPI, 2023-01-25) Nicolás Sánchez, Isabel; Almagro Romero, Lorena; Pedreño García, María Ángeles; Sabater Jara, Ana Belén; Biología VegetalMorus alba L. is used for a range of therapeutic purposes in Asian traditional medicine, and its extracts are reported to be effective against lipidemia, diabetes, and obesity, as well as being hepatoprotective and tyrosinase-inhibitory. They are also included in cosmetic products as anti-aging and skin-whitening agents. Stilbenes, the major bioactive compounds found in M. alba, have received renewed attention recently because of their putative activity against COVID-19. In this study M. alba plants were established in vitro, and the effect of elicitation on plant growth and stilbene accumulation, specifically oxyresveratrol and trans-resveratrol, was investigated. Different concentrations of the elicitors including methyl jasmonate and cyclodextrins were applied, and stilbene levels were determined in leaves, roots, and the culture medium. Elicitation of the M. alba plants with 5 mM cyclodextrins, alone or in combination with 10 µM methyl jasmonate, significantly increased the total phenolic content in the culture medium and leaves after 7 days of treatment. The higher total phenolic content in the roots of control plants and those treated only with methyl jasmonate indicated that cyclodextrins promoted metabolite release to the culture medium. Notably, the cyclodextrin-treated plants with the highest levels of oxy- and trans-resveratrol also had the highest total phenolic content and antioxidant capacity. These results indicate that elicited M. alba in vitro plants constitute a promising alternative source of bioactive stilbenes to supply pharmaceutical and cosmeceutical industries.
- PublicationOpen AccessChemoprevention of Experimental Periodontitis in Diabetic Rats with Silk Fibroin Nanoparticles Loaded with Resveratrol(MDPI, 2020-01-19) Giménez-Siurana, Ana; Gomez Garcia, Francisco; Pagan Bernabeu, Ana; Lozano-Pérez, Abel; Aznar-Cervantes, Salvador; Cenis, Luis; Lopez Jornet, Pia; Dermatología, Estomatología, Radiología y Medicina FísicaObjective: the objective of the present work is to study the e ectiveness of treatment with silk fibroin nanoparticles loaded with resveratrol in experimental periodontitis in a diabetic rat model. Introduction: Periodontitis is an inflammatory pathology highly related to other diseases, such as type II diabetes. Both diseases have a specific inflammatory condition, with Interleukin (IL)-6, IL-1 and Transforming Grow Factor (TGF)-1 being the most relevant proinflammatory factors. Silk fibroin (SF) nanoparticles loaded with resveratrol (Res-SFN) are a new alternative as a treatment. Methods: 40 diabetic Sprague Dawley male rats were used and periodontitis was induced by ligation. The animals were divided into 5 treatment groups, and 1 mL of treatment was administered once a day for 4 weeks. The groups were: I: Carboxymethyl cellulose (CMC) 0.8%, II: CMC 0.8% + SF 1%, III: CMC 0.8% + RES-SFN 3 mg/mL, IV: CMC 0.8% + SF 1% + RES-SFN 3 mg/mL, V: Water. A peripheral blood sample was taken every week to quantify the inflammatory profile by ELISA (IL-6, IL-1 and TGF-1 ). After 4 weeks the sacrifice was carried out and biopsies of the gum were taken. Results: Treatment with SF and RES-SFN reduced the amount of chemical inflammation mediators (with the exception of IL-1 in comparisons I-IV and II-IV (p > 0.05)), as well as the anatomopathological variables linked to it, in a significant way (p < 0.05). Conclusion: treatment with RES-SFN has reduced local inflammation in this experimental periodontitis model
- PublicationRestrictedComparative study of the antioxidant capacity of four stilbenes using ORAC, ABTS+, and FRAP Techniques(Springer, 2017-03-17) Rodríguez-Bonilla, Pilar; Matencio Durán, Adrián; Gandía Herrero, Fernando; López Nicolás, José Manuel; García Carmona, Francisco; Bioquímica y Biología Molecular "A"Researchers use several different analytical techniques, such as ORAC, ABTS·+, or FRAP, for measuring the antioxidant capacity of bioactive compounds. However, many authors do not take into account that these three techniques have different objectives. This contribution reports on the use of two types of tests to evaluate the antioxidant activity of four stilbene tests based first on the hydrogen atom transfer reaction (ORAC method) and the second on the single electron transfer reaction (ABTS·+ and FRAP assays). For the ORAC assay, the greatest antioxidant activity was shown by resveratrol, followed by oxyresveratrol, pterostilbene, and pinosylvin, while in the ABTS.+ assay, the highest antioxidant capacity was presented by oxyresveratrol, followed by resveratrol, pinosylvin, and finally pterostilbene. In the FRAP assay, the reducing activity shown by all the stilbenes was below that obtained for trolox. The role of phenolic hydroxyl groups was studied. The technique used should be selected taking into account the objectives and the conditions of the medium.
- PublicationOpen AccessConferencia: 'Resveratrol: nuevos horizontes para el tratamiento de enfermedades'(2011-02-24) Urbina, LuisConferencia: 'Resveratrol: nuevos horizontes para el tratamiento de enfermedades', a cargo de Mª de los Ángeles Pedreño García. Departamento de Biología Vegetal. Salón de Grados de la Facultad de Biología. Campus de Espinardo.
- PublicationOpen AccessEffects of four polyphenols on mouse wound healing and the gene expression profile of resveratrol action(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2024) Hu, Xiao; Zhang, Hanbin; Chen, ZhenguoStudies have demonstrated the potent effects of polyphenols on cutaneous wound healing. However, the molecular mechanisms underlying polyphenol activity are incompletely understood. Herein, mice were experimentally wounded, intragastrically treated with four polyphenols, resveratrol, tea polyphenols, genistein, and quercetin; and monitored for 14 days. Resveratrol was the most effective compound, promoting wound healing starting at day 7 after wounding, by enhancing cell proliferation and reducing apoptosis and subsequently promoting epidermal and dermal repair, collagen synthesis and scar maturation. RNA sequencing was performed in control and resveratrol-treated tissues on day 7 after wounding. Resveratrol treatment upregulated 362 genes and downregulated 334 genes. Gene Ontology enrichment analysis showed that differentially expressed genes (DEGs) were associated with different biological processes (keratinization, immunity, and inflammation), molecular functions (cytokine and chemokine activities), and cellular components (extracellular region and matrix). Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that DEGs were predominantly enriched in inflammatory and immunological pathways, including cytokine-cytokine receptor interaction, chemokine signaling, and tumor necrosis factor (TNF) signaling. These results show that resveratrol accelerates wound healing by promoting keratinization and dermal repair and attenuating immune and inflammatory responses.
- PublicationOpen AccessNanotechnology-based formulations for resveratrol delivery: Effects on resveratrol in vivo bioavailability and bioactivity(Elsevier Sci Ltd, 2019-04-15) Collado-González, Mar; Pereira, Irina; Pereira-Silva, Miguel; Ferreira, Laura; Caldas, Mariana; Magalhães, Mariana; Figueiras, Ana; Ribeiro, António J.; Veiga, Francisco; Santos, Ana Claudia; Biología Celular e HistologíaResveratrol (RES), also known as 3,5,4′-trihydroxystilbene, is a polyphenolic phytoalexin that has been widely researched in the past decade due to its recognized numerous biological activities. Despite the potential benefits of RES, its effective use is limited due to its poor solubility, photosensitivity and rapid metabolism, which strongly undermine RES bioavailability and bioactivity. Thereby, recently, nanotechnology appeared as a po- tential strategy to circumvent RES physicochemical and pharmacokinetics constrains. However, only few studies have addressed the crucial in vivo suitability of the developed delivery systems to improve RES efficacy. Facing this scenario, in the present review, it is intended to present and discuss the in vivo resveratrol bioavailability and bioactivity, following its encapsulation or conjugation in nanotechnology-based carriers, contemplating their pharmacokinetics effectiveness.
- PublicationOpen AccessOptimizing the preparation of silk fibroin nanoparticles and their loading with polyphenols: towards a more efficient anti-inflammatory effect on macrophages(MDPI, 2023-01-12) Ruiz Alcaraz, Antonio José; Núñez-Sánchez, María Ángeles; Asensio Ruiz, María Alejandra; Martínez-Sánchez, María Antonia; Oliva-Bolarín, Alba; Martínez Martínez, Teresa; Pérez Cuadrado, José Julián; Ramos-Molina, Bruno; Lozano-Pérez, Antonio Abel; Bioquímica y Biología Molecular B e Inmunología; Facultad de BiologíaSilk fibroin nanoparticles (SFN) have become a promising tool in drug delivery systems due to their physicochemical characteristics. SFN have shown their outstanding properties as an active vehicle for polyphenols, enhancing their antioxidant and anti-inflammatory effects on macrophages; therefore, it becomes necessary to have an easy, reproducible and scalable production method. In order to improve the production of nanoparticles, we performed direct precipitation of non-dialyzed silk fibroin solutions and evaluated the reproducibility of the method using dynamic light scattering. We also studied the loading efficiency of three different natural polyphenols using propylene glycol as a solvent. The loaded nanoparticles were fully characterized and used to treat human macrophage cells to assess the anti-inflammatory activity of these nanoparticles. The measured hydrodynamic characteristics of the SFN and the overall yield of the process showed that the new preparation method is highly reproducible and repeatable. Thus, we not only present a new scalable method to prepare silk nanoparticles but also how to improve the loading of natural polyphenolic compounds to the SFN, as well as the important anti-inflammatory effects of these loaded nanoparticles in a cell model of human macrophage cells.
- PublicationOpen AccessPolifenoles y diabetes: regulación fisiológica del metabolismo glucémico, el estrés oxidativo y la inflamación(2026-05-08) Victoria Montesinos, Desirée ; Mateo Orcajada, Adrián; FisiologíaLa fisiología de los polifenoles en la diabetes estudia cómo estos compuestos bioactivos presentes en alimentos vegetales pueden influir en el metabolismo de la glucosa y en la sensibilidad a la insulina. Los polifenoles pueden contribuir a reducir el estrés oxidativo y la inflamación, dos procesos implicados en el desarrollo y progresión de la diabetes. Además, pueden modular la función endotelial, la microbiota intestinal y algunas vías metabólicas relacionadas con la captación de glucosa y el equilibrio energético. En conjunto, su estudio permite comprender cómo ciertos componentes de la dieta podrían ayudar a mejorar la regulación metabólica y apoyar la prevención o el manejo de alteraciones glucémicas.
- PublicationOpen AccessResveratrol attenuates hepatic inflammation and oxidative stress in collagen-induced arthritis (CIA) mice via the Nrf2/Keap1 pathway(2026) Suhuan Chen; Haomiao Liu; Mengyan Zhang; Mengmeng Chen; Wuqi Chen; Guangyi Chen; Weilu Gao; Tao Yao; Xiaoyu Chen; Xuefei Fan1; Biología Celular e HistologíaBackground. Rheumatoid arthritis can affect extra-articular organs such as the liver, and the problem of drug-induced liver injury caused by traditional antirheumatic drugs for improving the condition cannot be ignored. This study aims to investigate the therapeutic effects of resveratrol (Res) on hepatic inflammation and oxidative stress in mice with collagen-induced arthritis (CIA) and to elucidate the relationship between the regulatory mechanisms of the nuclear factor erythroid 2 related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1) signaling pathway. Methods. In this study, we used chicken type II collagen in combination with complete Freund's adjuvant to induce arthritis in a mouse model, and Res was administered by tube feeding to detect the serum biochemical liver function and inflammation levels, oxidative stress, and apoptosis in the livers of mice. An in vitro cellular model of liver inflammation and oxidative stress was established by treating mouse primary hepatocytes (MPHs) with tumor necrosis factor α (TNF-α) and H2O2. The intrinsic mechanism of Res in attenuating hepatic inflammation and oxidative stress in CIA mice was explored by treating MPHs with an Nrf2 inhibitor and Keap1 overexpression plasmid. Results. Res significantly reduced the levels of inflammation and oxidative stress in liver tissues of CIA mice as well as in MPHs treated with TNF-α and H2O2 and activated the Nrf2/Keap1 signaling pathway. Inflammation and oxidative stress levels in MPHs were exacerbated by the use of Nrf2 inhibitors and Keap1 overexpression, which promoted apoptosis. Conclusion. This study demonstrated the intrinsic mechanism of Res of attenuating hepatic inflammation and oxidative stress in CIA mice through the Nrf2/Keap1 pathway, which provides a new idea for finding hepatoprotective treatments for rheumatoid arthritis.
- PublicationOpen AccessResveratrol can prevent CCl4-induced liver injury by inhibiting Notch signaling pathway(Universidad de Murcia. Departamento de Biología Celular e Histología, 2016) Tanriverdi, Gamze; Kaya-Dagistanli, Fatma; Ayla, Sule; Demirci, Sibel; Eser, Mediha; Unal, Seda; Cengiz, Mujgan; Oktar, HuseyinWe investigated whether Notch signaling was increased in an experimental liver fibrosis model and examined the effects of resveratrol on Notch expression. Rats were divided into four groups: the control group, injected with physiological saline; the CCl 4 group; the CCl 4 plus resveratrol group; and the resveratrol group. After treatment, immunostaining was performed to detect Notch1, Notch3, Notch4, transforming growth factor (TGF)-beta, alpha-smooth muscle actin (SMA), glial fibrillary acidic protein (GFAP), and proliferating cell nuclear antigen (PCNA), and TUNEL assays were performed to evaluate apoptosis. Sirius red staining was used to detect fibrosis. Samples were also biochemically evaluated for glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), lipid peroxidation, and protein oxidation. GSH, GPx, and catalase activities were significantly decreased (p<0.001) in the CCl 4 group. Distinct collagen accumulation was detected around the central vein and portal areas, and numbers of Notch1-, Notch3-, and Notch4-positive cells were significantly increased (p<0.001) in fibrotic areas in the CCl 4 group. Increased expression of Notch proteins in fibrotic areas may support the role of Notch in mediating signaling associated with liver fibrosis through activation of hepatic stellate and progenitor cells. In contrast, resveratrol prevented liver fibrosis by decreasing lipid peroxidation and may be effective for inhibiting Notch signaling.
- PublicationOpen AccessResveratrol decreases FoXO protein expression through PI3K-Akt-dependent pathway inhibition in H2O2-treated synoviocytes(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Xu, Bin; Wang, Gaoyuan; Zhang, Junqiang; Cao, Wei; Chen, XiaoyuThe aim of this study was to investigate the effects of resveratrol (Res) on hydrogen peroxide (H2O2)- treated fibroblast-like synoviocytes (FLSs) in vitro. We studied the phosphoinositide 3-kinase (PI3K)-Akt pathway inhibition-mediated effects of Res on forkhead box O (FoXO) mRNA expression levels. FLS viability was determined by Cell Counting Kit-8 (CCK-8) assay, and FLS apoptosis was measured by terminal deoxyribo nucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining and flow cytometry. FoXO1, FoXO3 and FoXO4 mRNA expression levels in FLSs were determined by RT-PCR, and p-Akt, Akt, p-FoXO1, FoXO1, p-FoXO3, FoXO3, Bcl-2 and Bax protein expression levels were determined by western blotting. Our results showed that low H2O2 concentrations (20 μM) can promote FLS growth and that Res significantly inhibited FLS activity. Moreover, Res significantly increased the number of apoptotic cells and the ratio of Bax/Bcl-2 protein expression in the group treated with Res compared with the group treated with H2O2 and LY294002, a PI3K inhibitor. Res also decreased FoXO1, FoXO3 and FoXO4 mRNA expression levels and pAkt/Akt, p-FoXO1/FoXO1, p-FoXO3/FoXO3 protein expression levels. Taken together, these findings indicate that Res can induce apoptosis in H2O2-treated FLSs in part by inhibiting the PI3K-Akt signaling pathway.
- PublicationOpen AccessThe inclusion complex of oxyresveratrol in modified cyclodextrins: a thermodynamic, structural, physicochemical, fluorescent and computational study(Elsevier, 2017-04-06) Matencio Durán, Adrián; López Nicolás, José Manuel; García Carmona, Francisco; Bioquímica y Biología Molecular A; Facultad de BiologíaThe interaction between oxyresveratrol (a type of stilbene with high biological activity) and modified cyclodextrins (CDs) was studied. Using HPLC-RP, was seen to form a 1:1 complex with all the CDs tested. The best CD in this respect was MβCD (KF = 606.65 ± 30.18 M−1), the complexation showing a strong dependence on pH and temperature: The complexation constant (KF) decreased as the pH and temperature increased. The thermodynamic parameters studied (ΔH°, ΔS° and ΔG°) showed negative entropy, enthalpy and Gibbs free energy change at 25 °C. In addition, fluorescence signal of oxyresveratrol increased when MβCD was added. The oxyresveratrol emission and excitation spectra were obtained for first time. A 1H NMR was carried out to study the structure of the complex and, DSC studied demonstrated the complexation. A computational study by molecular docking was made to complement the structural study.