Browsing by Subject "Rat aorta"

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    Enhanced expression of endothelin-1 and endothelinconverting enzyme-1 in acute hypoxic rat aorta
    (Murcia : F. Hernández, 2002) Doi, Y.; Kudo, H.; Nishino, T.; Yamamoto, O.; Nagata, T.; Nara, S.; Morita, M.; Fujimoto, S.
    Deeply anesthetized male Wistar rats were perfused by Hanks’ balanced salt solution bubbled with either 95%air and 5%CO2 (normoxic group) or 95%N2 and 5%CO2 (hypoxic group) from the thoracic aorta for 30 min, and the isolated abdominal aortae from both groups were used for electron microscopy, immunocytochemistry of endothelin (ET)-1 and ETconverting enzyme (ECE)-1, and in situ hybridization of p r e p r o E T-1 mRNA. A remarkable increase in the number of Weibel-Palade (WP) bodies, storage sites of E T-1 and ECE-1, occurred in the hypoxic group when compared to the normoxic group. Immunoreactivities for E T-1 and ECE-1, and signals for preproET-1 mRNA were seen along the endothelia of both groups, but the intensities were significantly elevated in the hypoxic group. The increase in the number of ECE-1 immunoreactive gold particles was noticed especially in WP bodies in the hypoxic group. These findings indicate the enhancement of preproET-1 synthesis in the aortic endothelial cells as well as the acceleration of ET- 1 processing in increased WP bodies in such cells in an experimentally hypoxic condition of the rat aortae.