Browsing by Subject "Pulpotomy"

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    Open Access
    Effect of N-Hexyl Cyanoacrylate, Calcium Hydroxide Plus Iodoform and Two Calcium-Silicate-Based Restorative Cements on Pulp Repair
    (OMICS international, 2018-09-28) Pérez Silva, Amparo; Serna Muñoz, Clara; Casimiro de Andrade, JD.; Pinhao Ferreira, A.; Ortiz Ruiz, Antonio José; Dermatología, Estomatología, Radiología y Medicina Física
    Objective: The objective of this study was to evaluate the histological response of rat molars to four materials placed on the dental pulp. Methods: We used 24 female Wistar rats (6 per group). The pulp chamber roof of the first and second upper molars was perforated. After control of bleeding (<5 minutes), a pulp coating material (ProRoot®MTA, IfabondTM, Calcipast+I or BiodentineTM) was placed. After 30 days of treatment, the molars were observed under an optical microscope. Results: ProRoot®MTA produced no inflammation of any pulp (p<0.0005). With Calcipast+I, inflammation was absent only in 18.7% of the pulps. All samples in all groups presented pulpal vitality. The ProRoot®MTA, IfabondTM and BiodentineTM groups showed a dentin bridge and reparative dentin in all pulps. Only ProRoot®MTA showed a completely regular odontoblastic layer (p<0.0005). IfabondTM and BiodentineTM generated an irregular odontoblastic layer in all pulps (p<0.00l) and Calcipast+I showed 50% regular and 50% irregular layers. All samples treated with ProRoot®MTA (p<0.0005), 81.25% with Calcipast+I and only 37.5% of those treated with IfabondTM and BiodentineTM (p<0.0005) showed fibrosis. In all groups, calcifications appeared in areas outside the dentin bridge (p=0.027). Conclusion: Although ProRoot®MTA had the best performance, the response of the pulp of rat molars to the materials used was similar according to the histological criteria of degree of inflammation and pulpal vitality. The regularity or irregularity of the odontoblastic layer did not influence the presence of a dentin bridge.
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    Open Access
    Histologic evaluation of the effect of grape seed oil and ferric sulfate in pulpotomy applied to molar teeth of rats: An in vivo study
    (2026) Günay Yapici Yavuz; Ebru Elibol Annaç; Aydın Keskinrüzgar; Muhammed Baybatmaz; İlknur Öz; Mesut Tozar; Osman Küçükkelepçe; Kamile Nur Tozar; Biología Celular e Histología; Universidad de Murcia, Departamento de Biologia Celular e Histiologia
    Background. The objective of this study was to examine the healing potential of Grape Seed Oil (GSO) on the pulp tissue that remains following pulpotomy treatment. Materials and Methods. The upper first molars of 18 two-month-old male Wistar albino rats were divided into two groups according to the material used in pulpotomy treatment. In the pulpotomy treatment group, GSO was used, while ferric sulfate (FS) was used in the control group. The rats were euthanized at 24 hours, 15 days, and 30 days following treatment. Histopathological evaluation of the samples was conducted using the hematoxylin-eosin staining method. The evaluation was conducted to ascertain alterations in connective tissue, vascular changes (i.e., angiopathic findings), inflammation, and hemorrhagic findings in the pulp. The data obtained in this study were analyzed with the IBM SPSS Statistics Version 21 software program. Results. In the present study, it was observed that the inflammatory response in the GSO group was higher than that in the FS group at 24 hours. The investigation revealed that the vascular response exhibited a higher magnitude in the GSO group compared to the FS group at 24 hours and at day 15. However, this response exhibited a decline at day 30. Despite an increase in hemorrhaging on the 15th day in the GSO group, this phenomenon decreased over time. Conclusions. It has been observed that GSO is a biocompatible material that can be used as an alternative to FS in pulpotomy treatment.