Browsing by Subject "Progesterone"
Now showing 1 - 8 of 8
Results Per Page
Sort Options
- PublicationOpen AccessDifferential expression of FGF family members in a progestin-dependent BT-474 human breast cancer xenograft model(F. Hernandez y JuanF. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología., 2012) López Pérez, Franklin R.; Liang, Yayun; Besch-Williford, Cynthia L.; Mafuvadze, Benford; Hyder, Salman M.Members of the fibroblast growth factor (FGF) family have been associated with tumor progression and angiogenesis, though the mechanism through which they affect the progression of breast cancer remains elusive. We recently showed that progestins increase the production of the potent angiogenic factor VEGF in an in vivo BT-474 human breast cancer cell-derived xenograft model. In this study we sought to determine the effect of progesterone (P) on regulation of specific FGF family members (FGF-2, FGF-4 and FGF-8) in the same model. Using immunohistochemistry we found that treatment with P significantly reduced FGF-2 and FGF-8 levels, while modestly increasing the levels of FGF-4 in tumors collected at the termination of the study or soon after P treatment began. The in vivo observations with FGF-2 were confirmed in cultured BT-474 cells, though the P-mediated reduction in FGF-2 was not blocked by the anti-progestin RU-486, suggesting that classical progesterone receptors (PR) are not involved in FGF-2 down-regulation. Also, P did not affect levels of FGF-2 mRNA in BT-474 cells, indicating that P exerts its effects on FGF-2 post-transcriptionally. Our observations suggest that the in vivo stimulation of BT-474 cell growth by P is associated with down-regulation of FGF-2 and FGF-8. Furthermore, since FGF-4 levels increased during P-treatment, FGF-4 may be required for tumor growth and maintenance and might therefore be a potential therapeutic target through which to suppress P-dependent tumor growth.
- PublicationOpen AccessEffect of cumulus cell removal and sperm pre‐incubation with progesterone on in vitro fertilization of equine gametes in the presence of oviductal fluid or cells(2019-05-30) Douet, C; Reigner, F; Goudet, G; Moros Nicolás, Carla; Biología Celular e HistologíaIn spite of many attempts to establish an in vitro fertilization (IVF) technique in the equine, no efficient conventional IVF technique is available. The presence of oviductal fluid or oviductal cells during IVF help to improve embryo production in vitro but is not sufficient to reach high fertilization rates. Thus, our aim was to perform equine IVF either after sperm preincubation with oviductal fluid or in the presence of oviductal cells, and to evaluate the effect of cumulus removal from the oocyte or sperm preincubation with progesterone. In experiment 1 and 2, IVF was performed in the presence of porcine oviduct epithelial cells. The removal of cumulus cells from equine oocytes after in vitro maturation tended to increase the percentage of fertilization when fresh sperm was used (1/33 vs 4/31, p > 0.05) but had no effect when frozen sperm was used (1/32 vs 1/32). Equine sperm preincubation with progesterone did not significantly influence the fertilization rate when fresh or frozen sperm was used (2/14 vs 2/18 for fresh, 1/29 vs 1/25 for frozen). In experiment 3 and 4, IVF was performed after preincubation of sperm with porcine oviductal fluid. The removal of cumulus cells tented to increase the percentage of fertilization when fresh sperm was used (1/24 vs 3/26, p>0.05). Sperm preincubation with progesterone did not significantly influence the fertilization rate when fresh or frozen sperm was used (2/39 vs 2/36 for fresh, 2/37 vs 1/46 for frozen), but two 3-4 cells stage zygotes were obtained with fresh sperm preincubated with progesterone. This is an encouraging result for the setting up of an efficient IVF procedure in equine.
- PublicationOpen AccessEffects of progesterone on the ultrastructure of the golden hamster parathyroid gland(Murcia : F. Hernández, 1995) Emura, S.; Hayakawa, D.; Yamahira, T.; Terasawa, K.; Tamada, A.; Arakawa, M.; Isono, H.; Shoumura, S.The ultrastructure of the parathyroid glands in golden hamsters after administration of progesterone was investigated. In the parathyroid glands of the female hamsters after administration of progesterone, the mean serum calcium concentration was significantly higher when compared to that of the control hamsters. In the male experimental hamsters, the percentage area occupied by Golgi complexes and lipid droplets was significantly increased when compared to that of the control hamsters, respectively. In the female hamsters after administration of progesterone, the percentage area occupied by large vacuolar bodies was significantly decreased when compared to that of the control hamsters. In the male and female experimental hamsters, the mean number of secretory granules per 100 pm2 of cytoplasm showed a significant increase compared with that of the male and female control hamsters, respectively. These findings suggest that the secretory activity of the parathyroid glands may be stimulated in response to hypocalcemia induced by progesterone.
- PublicationRestrictedEffect of Estradiol and Progesterone on Metabolic Biomarkers in Healthy Bitches(Wiley , 2013) Carrillo Sánchez, J. D.; Tvarijonaviciute, Asta; Cerón Madrigal, José Joaquín; Medicina y Cirugía Animal; Facultades de la UMU::Facultad de VeterinariaEl objetivo de este estudio fue evaluar la posible asociación entre los cambios hormonales que ocurren durante el estro y los biomarcadores relacionados con el metabolismo de la glucosa (glucosa e insulina), el metabolismo lipídico (perfil lipídico y BChE) y las adipocinas (adiponectina y grelina) en perras sanas. Con este propósito, se midieron estos analitos en suero de perras en dos momentos: antes (T1) y después (T2) del pico de LH, establecidos según las concentraciones de progesterona. Se observaron niveles aumentados de colesterol total (p < 0,01), colesterol unido a lipoproteínas de alta densidad (HDL-C) (p < 0,01), colesterol unido a lipoproteínas de baja densidad (LDL-C) (p < 0,01), adiponectina (p < 0,01) y grelina (p < 0,05) en T2 en comparación con T1. No se observaron cambios estadísticamente significativos en la glucosa sérica, insulina, modelo de evaluación de la homeostasis para la sensibilidad a la insulina (HOMA), triglicéridos ni en la BChE. Cuando se analizaron conjuntamente los datos de T1 y T2, la adiponectina sérica mostró correlación positiva con la progesterona (r = 0,353; p = 0,022) y con HDL-C (r = 0,307; p = 0,048), y correlación negativa con la insulina (r = -0,429; p = 0,005), con HOMA (r = -0,446; p = 0,003) y con BChE (r = -0,522; p < 0,001). La grelina mostró correlación negativa con el estradiol (r = -0,701; p = 0,004). La BChE se correlacionó negativamente con el estradiol (r = -0,441; p = 0,018) y con la glucosa (r = -0,343; p = 0,028), y positivamente con la insulina (r = 0,460; p = 0,003) y con HOMA (r = 0,505; p < 0,001). En conclusión, tras el pico de LH se producen cambios en los biomarcadores metabólicos en perras, caracterizados por un aumento de los lípidos (colesterol total, colesterol HDL y colesterol LDL) sin cambios en la actividad de BChE, así como un incremento en las concentraciones de adiponectina y grelina, sin cambios significativos en la glucosa ni en la insulina.
- PublicationOpen Accesslmmunohistochemical localization of prolactin in functioning and regressing corpus luteum of pituitary autotransplanted rats(Murcia : F. Hernández, 1986) Martín de las Mulas, J.; Aguilar, E.; Sánchez-Criado, J.E.In an attempt to shed light on the intimate mechanism by which prolactin (PRL) switches from supporting corpus luteum (CL) progesterone secretion (P) to promote structural regression of the CL, day 2 (metestrous) autopituitary transplanted (APTr) rats were used. In APTr rats the CL is under the only control of PRL since an almost complete absence of LH and FSH exist. The experimental group was given bromocriptine (CB-154: 0.4 mg/day) on days 12, 13 and 14 of the cycle and 0.25 ml of ethanol from day 15 to day 21. The control group was given CB-154 from day 12 to day 21. Rats were hemiovariectomized on day 12 to assess the morphological characteristics of the active CL. PRL and P were determined by RIA on days 12, 15 and 22. On day 12, both PRL and P levels were higher than 80ng/ml (luteotrophic action of PRL). On day 15, due to treatment with CB- 154, the levels of both hormones had fallen below 7 ng/ml (functional luteolysis). On day 22, PRL levels were again high ( > 50 ng/ml) in the shortly CB-154-treated rats and low ( < 5 ng/ml) in the controls; the P levels were lower than 5 ng/ml in both groups. PRL-induced structural luteolysis in the experimental group (hyperprolactinemic) was assessed by the structural characteristics and by the CL weight loss on day 22 in comparison with that exhibited by control rats. The immunohistochemical staining of both endogenous and total PRL in the lutein cells showed that the internalization of PRL is not modified by the functional state of the CL, nevertheless the intracellular redistribution of the internalized hormone varied in relation with the PRL action on the CL (luteotrophic, day 12vs luteolytic, day 22).These results seem to indicate that intracellular mechanisms rather than receptor content determine CL response to PRL.
- PublicationOpen AccessParticular functions of estrogen and progesterone in establishment of uterine receptivity and embryo implantation(Murcia : F. Hernández, 2010) Ozturk, Saffet; Demir, RamazanThe process of embryo implantation requires synchronized development of blastocyst and timely establishment of uterine receptivity. Establishment of uterine receptivity, preimplantation embryo development and embryo implantation events are mainly regulated by certain factors, including cytokines, chemokines, growth factors and steroid hormones. Recent studies suggest that steroid hormones, especially estrogen and progesterone, play important roles in supporting endometrial preparations to establish endometrial receptivity. Timely establishment of endometrial receptivity is a crucial process for providing successful embryo implantation. Although many investigations until now have been performed to precisely understand the effects of estrogen and progesterone on acquiring uterine receptivity and embryo implantation in humans and rodents, there are limited numbers of studies that largely focus on this subject. Therefore, in this article we discuss the studies associated with significant functions of estrogen and progesterone in establishing receptive endometrium and the process of embryo implantation in humans and rodents.
- PublicationOpen AccessPresente y futuro del diagnóstico de gestación en el ganado bovino(Universidad de Murcia. Servicio de publicaciones, 2022) Sice, Margaux; Gómez Martín, Ángel; Gomis Almendro, JesúsPara llevar a cabo el diagnóstico de gestación en el ganado bovino, se debe utilizar un método preciso, seguro, económico y que se pueda realizar de manera temprana. Aunque varias técnicas están actualmente disponibles en el mercado, otras siguen todavía en desarrollo, siendo posibles herramientas diagnósticas a tener en cuenta en un futuro. Por ello, el presente trabajo tiene como objetivo hacer una revisión sobre las diversas técnicas de diagnóstico de gestación (DG) y su potencial uso a nivel comercial, tanto en el presente como en el futuro, de la ganadería bovina. Los métodos directos para el DG, como son la palpación directa o la ecografía del tracto reproductor vía transrectal, siguen siendo los más empleados en la actualidad durante el control gestacional en el ganado vacuno. Son técnicas diagnósticas con buenos resultados y son interesantes a nivel económico, pero son herramientas invasivas y requieren una cierta experiencia por parte del técnico que las realiza. En general, se aplican a partir de la 3ª-4ª semana posterior a la inseminación artificial. Por otro lado, los métodos indirectos de DG son menos invasivos que los directos. Mediante este tipo de diagnósticos vía indirecta se puede detectar la presencia o ausencia de un embrión, sin visualizar directamente estructuras gestacionales. Existen métodos indirectos basados en signos clínicos, como la vigilancia del retorno al estro, así como técnicas indirectas bioquímicas, que permiten evaluar mediante el uso de kits rápidos ciertas sustancias como la progesterona (P4) o las Glicoproteínas Asociadas a la Gestación (GPAG), producidas durante la gestación de forma temprana. Otras técnicas novedosas y prometedoras, pero que todavía se encuentran en desarrollo, sonla evaluación de moléculas como el interferón tau (IFNτ), los micro-ARN (miARN) y/o los Factores de Gestación Temprana (FGT).
- PublicationOpen AccessThe antiprogestins mifepristone and onapristone reduce cell proliferation in the canine mammary carcinoma cell line CMT-U27(F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología, 2014) Guil Luna, Silvia; Hellmén, Eva; Sánchez Céspedes, Raquel; Millán, Yolanda; Martín de las Mulas, JuanaCanine mammary tumours (CMTs) represent nearly half of all tumours in female dogs and some 50% have malignant behaviour. Simple epithelial carcinomas have shorter disease free periods after surgery and a higher reduction of the proliferation index reduction after antiprogestin aglepristone treatment in vivo related to the expression of progesterone receptors (PR). These findings make simple carcinomas good candidates for endocrine therapy. To further explore this possibility, the effects of the antiprogestins mifepristone (RU486) and onapristone (ZK299) on cell viability and PR expression of the canine mammary carcinoma cell line isolated from a simple epithelial carcinoma CMT-U27 were studied. Twenty five percent of CMT-U27 control cells expressed PR. RU486 (p<0.05) and ZK299 (p<0.05) reduced the number of viable cells (WST-8 test) at 24h but only the latter treatment reduced significantly PR expression in viable tumour cells at 24h of incubation. The results suggest that both RU486 and ZK299 induce a decrease in the number of viable CMT-U27 tumour cells with different effects on PR expression. The canine mammary carcinoma cell line CMT-U27 is sensitive to the effects of antiprogestins and may serve to further explore the role of these drugs in canine mammary carcinomas.