Browsing by Subject "Platelet rich plasma"

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    Influence of age and sex on platelet count: implications for optimizing growth factor-rich plasma preparation
    (Medicina Oral, 2025-07-22) Sánchez Pérez, Arturo Joaquín; Palma Sánchez, Ana; Jornet García, Alfonso Felipe; Moya Villaescusa, María José; Montoya Carralero, José María; Dermatología, Estomatología, Radiología y Medicina Física
    Background: Growth factor-rich plasma (GFRP) is a biomedical procedure used to promote tissue regeneration. Platelet-rich plasma (PRP) contains higher-than-average platelet concentrations and includes platelet-derived growth factors. As an autologous blood derivative, it is widely used to enhance healing and tissue regeneration . In dentistry, GFRP accelerates soft tissue healing and bone regeneration in procedures such as complex extractions, bone grafts, cyst treatments, and to improve dental implant osseointegration. Objective: To assess the potential influence of age and sex on platelet count, with the aim of optimizing the standardization of therapies involving growth factors. Material and Methods: A cross-sectional, observational, and comparative study was conducted, including a total of 384 patients. Participants were stratified into six groups based on age. From their medical records, the platelet counts obtained from analyses meeting the established inclusion criteria were recorded. Subsequently, the distribution of platelet levels was analyzed according to age groups and sex. Additionally, the potential correlation between platelet count and age was evaluated. Results: The mean platelet count was higher in women (260.9 ± 65.9 x10³/μL) than in men (250.4 ± 62.0 x10³/μL), although the difference was not statistically significant (p = 0.113). A statistically significant negative correlation was found between age and platelet count (r = -0.175; p < 0.01), indicating that platelet count decreases with age, although the correlation coefficient was low. Conclusions: Platelet count varies across the population; therefore, blood extraction for PRP preparation should be adjusted based on age group and sex.
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    PRP and MSCs on tenocytes artificial wound healing: an in vitro study comparing fresh and frozen PRP
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2018) Veronesi, Francesca; Pagani, Stefania; Torricelli, Paola; Filardo, Giuseppe; Cavallo, Carola; Grigolo, Brunella; Fini, Milena
    Tendon tissue has poor regenerative capacity due to its low vascularization, cell density and extracellular matrix (ECM) production. Therefore, tendon injuries are an increasing clinical problem because of the formation of scar tissue with traditional therapies. Regenerative medicine aims at triggering a healing response through the use of biological treatments such as mesenchymal stromal cells (MSCs) and growth factors (GFs). MSCs show several advantages in tendon clinical setting, while platelet rich plasma (PRP) has gained popularity because of its high GF concentration, although its applications in the tendon clinical setting are still controversial. The aim of the present study was to evaluate a combined treatment of MSCs and PRP in an in vitro microwound model of tendon injuries. In addition, fresh and frozen PRP were compared. Single human tenocytes cultures or co-cultures with bone marrow derived MSCs (BMSCs) were set up with or without human PRP, fresh or frozen. After 24 hours of culture, it was observed that MSCs alone significantly increased tenocyte migration speed, microwound healing rate, fibronectin, collagen I and aggrecan production. These effects were enhanced by the combination with PRP, fresh being more effective than frozen PRP. In addition, the number of MSCs and tenocytes inside the microwound was significantly increased, especially with fresh PRP. In conclusion, the combination of MSCs and PRP, especially the fresh one, increases tenocytes and MSC migration speed, as well as ECM protein production compared to the use of MSCs alone