Browsing by Subject "Ovine"

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    Associated risk factors influencing ovine Eimeria infections in southern Spain
    (Elsevier, 2018) Carrau Garreta, Tessa; Silva, L.M.R.; Pérez, D.; Failing, K.; Martínez Carrasco-Pleite, Carlos; Macías, J.; Taubert, A.; Hermosilla, C.; Ruiz de Ybáñez Carnero, María del Rocío; Sanidad Animal
    Eimeria infections are common in sheep industry worldwide nonetheless very little is known on infection dynamics and associated risk factors influencing the outcome of clinical ovine coccidiosis. The aim of the present work was to analyse intrinsic (e. g. host age) and extrinsic (flock size, farm size and animal density) risk factors influencing dynamics of Eimeria spp. infections in sheep farms of the semi-arid region of Murcia in southern Spain. Overall, 64 ovine flocks in Murcia were surveyed and a total of 396 faecal samples were collected between May and July 2015. Flotation and McMaster techniques were performed for parasitological investigation. Excreted oocysts were identified on the basis of morphological as well as morphometric characteristics. In all analysed samples Eimeria spp. oocysts (100%) were found and 11 different Eimeria species were identified. Most prevalent species was E. ovinoidalis (97%) followed by E. parva/E.pallida (97%), E. weybridgensis (90.9%) and E. crandallis (89.4%), respectively. Concomitant infections with more than one Eimeria species were observed in all analysed samples. Analysis of intrinsic/extrinsic risk factor revealed age, herd/farm size and animal density to be the main factors significantly influencing Eimeria oocysts per gram of faeces counts (OPG) and overall prevalence of Eimeria spp. infections in sheep.
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    Cestodosis larvarias en el ganado ovino de la región de Murcia (sureste de España)
    (2005-01-01) Garijo Toledo, M. Magdalena; Alonso de Vega, Francisco; Martínez Carrasco-Pleite, Carlos; Ruiz de Ybáñez Carnero, María del Rocío; Sanidad Animal
    El presente estudio se realiza con la intención de conocer el estado parasitario del ganado ovino adulto de la región de Murcia (Sureste español) en relación a las cestodosis larvarias. Para tal fin, se examinaron un total de 151 ovejas adultas sacrificadas en un matadero regional, de las cuales tres (1,67%) presentaban Coenurus cerebralis en el hemisferio cerebral izquierdo, un total de 18 animales (9,94%) mostraron la presencia de Cysticercus tenuicollis, vesiculas siempre localizadas en membranas serosas de la cavidad abdominal y en la superficie hepática, mientras que ningún animal demostró la presencia de quiste hidatídico alguno.
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    Comparison of chromaffin cells from several animal sources for their use as an in vitro model to study the mechanism of organophosphorous toxicity
    (Elsevier, 2006-04-28) Romero, D.; Quesada, E.; Sogorb, M. A.; Vilanova, E.; Carrera, V.; García Fernández, Antonio Juan; Ciencias Sociosanitarias
    It had been observed that the chromaffin cells of bovine adrenal medulla contain high levels of neuropathy target esterase (NTE), the esterase whose inhibition and aging is associated with induction of the organophosphorous induced delayed neuropathy. In this study, total esterase and NTE activities, and their inhibition kinetics by OPs are characterized in adrenal medulla of several species in order to find the best source for chromaffin cells. Total esterase activity in membrane fraction of bovine, equine, porcine, ovine and caprine were 6100±840, 4200±270, 5000±120, 28800±3000, and 10800±2400 mU/g tissue, respectively (mean±S.D., n = 3–4). NTE represented around 70%, 24%, 58%, 10% and 24% of the total esterases in the same tissues, respectively. It was deduced that NTE represents between 69% and 89% of the “B-activity” (activity resistant to 40 M paraoxon) in the membrane fraction of all species. The mipafox I50 calculated for 30-min inhibition of NTE at 37 ◦C ranged between 7.4 and 12 M. These values are in the range of that for brain NTE in hen (the usual model for testing OP delayed neurotoxicity). Considering that bovine adrenal medulla contains high NTE activity, that it represents a high proportion of total activity, it is easier to dissect than adrenal medulla from equine, caprine or ovine, and is more readily available than species cited previously, and that its inhibitory properties are similar to the classical hen brain model, it is deduced that bovine adrenal medulla is the most appropriate source of chromaffin cells to study OP toxicity, with porcine as the second alternative. The kinetic properties of chromaffin cell cultures from bovine and porcine were in accordance with their properties in homogenate and subcellular fractions, and they displayed an appropriate stability and viability of the primary culture to be used in in vitro toxicological studies for both mechanistic and testing purposes.
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    Empleo de ivermectina como parasiticida en ovino: posibles efectos tóxicos y repercusiones ambientales
    (Murcia: Servicio de Publicaciones de la Universidad de Murcia, 2011) García Salazar, B.; Hernández Moreno, D.; Soler Rodríguez, F.; Pérez López, M.
    El profesional veterinario debe conocer no sólo los efectos terapéuticos derivados del empleo de ciertos agentes farmacológicos, sino también los posibles riesgos medioambientales que su actividad puede ocasionar. A través del presente trabajo se repasan las enormes posibilidades terapéuticas de la ivermectina como antiparasitario en el ganado ovino, pero también se hace hincapié en sus efectos ambientales, intentando concienciar al veterinario acerca de su importante papel como profesional implicado en la protección del medio ambiente.
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    First description of an in vitro culture system for Eimeria ovinoidalis macromeront formation in primary host endothelial cells
    (Elsevier, 2016-05-25) Carrau Garreta, Tessa; Machado Ribeiro Silva, Liliana; Pérez, David; Ruiz de Ybáñez Carnero, María del Rocío; Taubert, Anja; Hermosilla, Carlos; Sanidad Animal
    The apicomplexan parasite Eimeria ovinoidalis is distributed worldwide and causes clinical ovine coccidiosis. As one of the most pathogenic species in sheep, the principal clinical sign is profuse diarrhoea in young animals, which leads to important economic losses in the ovine industry. We here aimed to establish an in vitro culture system for the development of E. ovinoidalis macromeronts, as no suitable systems are currently available for any ovine Eimeria species. Faecal samples containing more than 90% of E. ovinoidalis oocysts were collected from naturally infected lambs and ewes in Murcia Region (Spain). E. ovinoidalis oocysts were collected, left to sporulate in potassium dichromate and stored at 4 °C until further studies were conducted. Moreover, a suitable excystation protocol was effectively established, resulting in the release of viable sporozoites, which were allowed to infect primary bovine umbilical vein endothelial cells (BUVEC) and permanent bovine colonic epithelial cells (BCEC). In vitro first merogony was successfully accomplished exclusively in BUVEC leading to macromeront formation (up to 100 μm) and the release of fully developed and viable merozoites I stages. Given that we were able to establish a suitable in vitro system for the first merogony of such pathogenic Eimeria species in sheep, advances might be further made not only on studies regarding the control of ovine coccidiosis, such as drug screenings, but also on the better understanding of molecular parasite–host cell interactions as already demonstrated for other ruminant Eimeria species.
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    Lesions in lambs experimentally infected
    (Murcia : F. Hernández, 1995) Masot, A.J.; Gázquez, A.; Regodón, S.; Franco, A.; Redondo, E.
    An experimental model was designed to characterize lesions in the lung of lambs inoculated with bovine respiratory syncytial virus (BRSV). 25 Merino lambs of both sexes, with a live weight of 17+3 Kg, received an intratracheal inoculation of 20 m1 saline solution containing 1 . 2 6l~o 6 TCIDSOB RSV (strain NMK-7) per ml. Lambs were laughtered 1, 3, 7, 11 and 15 postinoculation days (PID), and histopathological, immunohistochemical and electron microscopic studies were performed. Results reflected a series of lesions, the most noteworthy of which were bronchiolitis obliterants with destruction of the mucociliary apparatus, the presence of syncytial cells in alveoli and a progressive interstitial reaction. BRSV antigen was detected in lung samples. These changes might be expected to decrease the efficiency of respiratory tract defence mechanisms, rendering the lung parenchyma susceptible to opportunist bacteria1 infection.
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    Nematodosis broncopulmonares en el ganado ovino de la región de Murcia (sureste de España).
    (2007-01-01) Garijo Toledo, M. Magdalena; Alonso de Vega, Francisco; Martínez Carrasco-Pleite, Carlos; Ruiz de Ybáñez Carnero, María del Rocío; Sanidad Animal
    El presente trabajo analiza la presencia de nematodos broncopulmonares en el ganado ovino adulto de la Región de Murcia (Sureste español). Se examinaron un total de 181 ovejas adultas sacrificadas en un matadero regional. El 72,4% de los animales estaban parasitados por alguna de las siguientes especies, citadas por orden de prevalencia: Cystocaulus ocreatus (67,7%), Neostrongylus linearis (63%), Muellerius capillaris (42,5%), Dictyocaulus filaria (7,7%) y Protostrongylus rufescens (4,7%). Se discute la prevalencia de estas nematodosis entre las diferentes comarcas ganaderas de la Región de Murcia.
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    Particularidades etiológicas y epidemiológicas de la agalaxia contagiosa ovina: similitudes y diferencias con el caprino
    (Murcia: Servicio de Publicaciones de la Universidad de Murcia, 2013) Prats-van der Ham, M.; Gómez-Martín, A.; Tatay-Dualde, J.; Paterna, A.; Amores Iniesta, Joaquín; Corrales Romero, Juan Carlos; Contreras de Vera, Antonio; Sánchez, A.; De la Fe Rodríguez, Christian; Sanidad Animal
    Este trabajo describe las diferencias existentes en la presentación de la agalaxia contagiosa, síndrome infectocontagioso causado por varias especies del genero Mycoplasma spp., en el ganado ovino y caprino. Las particularidades etiológicas y epidemiológicas de la infección crónica en la cabra, y la ausencia de datos similares en el ovino, evidencian la necesidad de realizar nuevos trabajos que determinen si las diferencias observadas en referencia a la presencia de portadores auriculares en los rebaños o la participación de los sementales en la difusión de la enfermedad son fruto de la escasez de trabajos científicos o por el contrario, establecen verdaderas diferencias sobre las que desarrollar estrategias dirigidas de control en función de la especie de rumiante afectada.
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    Presence of pigment in the ovine pineal gland during embryonic development
    (F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología, 1998) Regodón, S.; Franco, A. J.; Gazquez, A.; Redondo, E.
    Light- and electron-mi croscopi c analyses were used 10 id entify and desc ribe th e charac teristi c features of cells containing pigment in the ov ine pineal gland during prenatal deve lopment. 72 ov ine embryos (36 male, 36 female) ranging in age from 54 to 150 days were used fo r this study. Ce lls co nt aining pigment granules were a constant fea ture in this gland . Wh en samples from the di f'ferent groups were inspected using the naked eye, the detection of pigment was of 61 % in groups I (54 to 67 days of prenatal development) and II (7 1 to 92 days of prenatal development), ~3 % in group III (9t; to 113 days of prenatal development) and 25% in gro up IV (II R to 150 days of prenatal deve lopment). The morphologica l featu res and histochemical properties of the pineal pigment enab led it to be identified as me lanin . Several types o f pigme nt granules were ultrastru cturall y distinguished; th ese varied in size, shape and loca tion within pinea l cell populations. The pigment g ranul es were de tec ted in pine alocy te s, interstitial cells and pigmented ce lls. The largest amount and the widest variety of pigmented granules were found in pigment ed ce lls. The prese nce of cells containing pigmented gra nules amongst the cell populations of the developing ov in e pineal gland was a nal ysed a nd compared with that of other mammalian species.
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    La trashumancia del ovino segureño: una oportunidad para la innovación docente en veterinaria
    (Universidad de Murcia. Servicio de publicaciones, 2021) Contreras de Vera, Antonio; Sánchez López, Antonio; Corrales Romero, Juan Carlos; García Galán, Ana; García Romero, Edgar; Rodríguez; Fé Rodríguez, David Christian de la; Aldeguer Aldeguer, María Paz; Gómez Martín, Ángel
    La importancia de la producción de cordero en el Sureste, la existencia de un núcleo de trashumantes de ovino en la Sierra de Segura, y la consideración de la trashumancia como manifestación representativa del patrimonio cultural inmaterial nos ha llevado a explorar el potencial docente de la trashumancia con ovino segureño para estudiantes de veterinaria. Entre 2016 y 2019, cuatro profesores y dieciocho estudiantes de veterinaria de las Universidades de Murcia y Cardenal Herrera (UCH-CEU) de Valencia, hemos realizado diecinueve jornadas de trashumancia. Entre las acciones de innovación docente generadas destaca la exposición fotográfica “La Vereda de los Pontoneros” y el documental “Trashumancia, la Última Frontera”. Proponemos la integración de una semana de trashumancia en el periodo de las dos semanas de prácticas tuteladas con la Asociación Nacional de Criadores de Ovino Segureño (ANCOS), discutimos su integración en el grado de Veterinaria de Murcia y evidenciamos el interés docente de esta actividad inmersiva, que supera los propios contenidos del grado de veterinaria