Browsing by Subject "Nerve regeneration"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Publication
    Open Access
    Biological and biocompatible characteristics of fullerenols nanomaterials for tissue engineering
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2021) Zhao, Yizhe; Shen, Xinyuan; Ma, Ruimeng; Hou, Yiting; Qian, Yun; Fan, Cunyi
    . Fullerenes, as hydrophobic molecules, are limited in biomedical function due to their very low solubility. But taking C60(OH)x as an example, the properties of fullerenols were analyzed. It was found that fullerenols had good stability, water solubility, good biocompatibility and low cytotoxicity by adding a hydroxyl group to carbon atoms. In the biomedical field, it has been found that fullerene C60 can be used as a powerful free radical scavenger, with antioxidant activity, with antibacterial and inhibitory effects on cancer cells. Fullerenols inherit the good properties of fullerenes, and are better used in cancer treatment, including loading drug therapy and directly as an anticancer drug. In addition, fullerenols are also used in the repair of myocardial injury, the treatment of myocardial infarction and neuroprotection. With the development of tissue engineering technology, the preparation of nerve scaffolds which can improve ischemia, hypoxia and oxidative stress after nerve injury has become a research hotspot. The electron absorption and reduction characteristics of fullerenols in biomedical research bring new ideas for the treatment of oxidative stress in the repair of peripheral nerve defects. It seems that the research on fullerenols loaded neural scaffold has great prospects.
  • Thumbnail Image
    Publication
    Open Access
    Ghrelin and adipose-derived mesenchymal stromal cells improve nerve regeneration in a rat model of epsilon-caprolactone conduit reconstruction
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Hernández Cortés, Pedro; Toledo Romero, Miguel Angel; Delgado, Mario; Gonzalez Rey, Elena; Gómez Sánchez, Rafael; Prados Olleta, Nicolás; Aneiros Fernández, José; Crespo Lora, Vicente; Aguilar, Mariano; Galindo Moreno, Pablo; O’Valle, Francisco
    Objective. Attempts have been made to improve nerve conduits in peripheral nerve reconstruction. We investigated the potential therapeutic effect of adipose-derived mesenchymal cells (ASCs) and ghrelin (GHR), a neuropeptide with neuroprotective, trophic, and developmental regulatory actions, on peripheral nerve regeneration in a model of severe nerve injury repaired with nerve conduits. Material and methods. The right sciatic nerves of 24 male Wistar rats were 10-mm transected unilaterally and repaired with Dl-lactic-ε-caprolactone conduits. Rats were then treated locally with saline, ASCs, or GHR. At 12 weeks post-surgery, we assessed limb function by measuring ankle stance angle and percentage muscle mass reduction and evaluated the histopathology, immunohistochemistry, ultrastructure, and morphometry of myelinated fibers. Main Results. Rats receiving GHR or ASCs showed no significant increased functional recovery in ankle stance angle (p=0.372) but a higher nerve area (p=0.015), myelin area (p=0.046) and number of myelinated fibers (p=0.012) in the middle and distal segments of operated sciatic nerves in comparison to saline-treated control animals. Conclusion. These results suggest that utilization of ghrelin or ASCs may improve nerve regeneration using Dl-lactic-ε-caprolactone conduits.
  • Thumbnail Image
    Publication
    Open Access
    Peripheral nerve injury and regeneration
    (Murcia : F. Hernández, 1995) Terenghi, G.
    The process of nerve regeneration has been studied extensively by traditional morphological methods, but it is only recently that has been possible to identify more precisely the contribution of different nerve subpopulations. By studying different models of nerve repair and regeneration, it is becoming apparent that other tissue components are contributing to the overall process. When muscle grafting is carried out to repair an injured nerve, the regenerating axons are migrating in parallel with Schwann cells to bridge the nerve gap. The presence of Schwann cells is essential for a successful nerve regeneration, most probably because their production of different neuronal trophic factors. This pattern is also repeated when fibronectin mats are used for nerve repair, indicating the possibility to use this new synthetic matrix for clinical application. If the target organ is analysed after nerve repair, the recovery of all nerve components is evident. However, the process occurs at different times in separate s k i compartments, and the regeneration of the autonomic innervation appears to be preceded by that of the sensory nerves. When looking at cutaneous nerve regeneration following different type of injury, a common pattern of events becomes apparent. In skin flaps, nerve regeneration begins from the skin surrounding the wound edge, or from the pedicle, and sensory nerves are the first to penetrate into the flap. Angiogenesis precedes reinnervation of the flap, and initially regenerating fibres appear to be associated with newly formed blood vessels. This pattern is evident also in full-thickness wounds and in suction blisters, where only the more superficial cutaneous layer is disrupted. Furthermore, the presence of keratinocytes appears to exert a directional influence on both regenerating blood vessels and nerves, which follow the regenerating keratinocytes when reepidermalisation is taking place. These results would indicate that there is a close relationship between nerve fibres and blood vessels during regeneration, with a substantial contribution to the process from other tissue components and soluble factors from the surrounding environment.