Browsing by Subject "Myoepithelial cell"

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    Diagnostic utility of CD205 in breast cancer: Simultaneous detection of myoepithelial cells and dendritic cells in breast tissue by CD205
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Ohe, Rintaro; Aung, Naing Ye; Tamura, Yuka; Kabasawa, Takanobu; Utsunomiya, Aya; Tamazawa, Nobuyuki; Kitaoka, Takumi; Meng, Hong-Xue; Shibata, Kenichi; Yamakawa, Mitsunori
    Background. CD205 can be used to detect myoepithelial cells (MECs) and dendritic cells (DCs) in breast tissue. However, the usefulness of CD205 immunostaining in the pathological diagnosis of breast tumors is not fully understood. The objective of this study was to re-evaluate CD205 co-expression with other MEC markers, such as p63 and CD10, in nonneoplastic and neoplastic breast tissue and to evaluate its pathological diagnostic utility in these types of breast cancer. Material and methods. Nonneoplastic breast tissue samples with a terminal duct lobular unit and duct were obtained from fibroadenoma and mastopathy patients. Neoplastic breast tissue samples included ductal carcinoma in situ (DCIS) (n=43) and invasive ductal carcinoma (IDC) (n=60), including the tubule-forming type (n=20). These specimens were investigated by CD205, p63, and CD10 immunostaining. Results. In addition to p63 and CD10, CD205 was expressed on MECs in nonneoplastic breast and DCIS tissue samples; CD205 was simultaneously detected on DCs that had infiltrated DCIS and IDC tumor nests. CD205 was expressed on cancer cells themselves in only 7.3% of the breast cancer samples. The number of intratumoral CD205 + DCs in tubular IDC was significantly higher than that in DCIS (P<0.01)
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    Morphological changes of myoepithelial cells of mouse lacrimal glands during postnatal development
    (Murcia : F. Hernández, 1995) Wang, Y.L.; Tan, Y.; Satoh, Y.; Ono, K.
    To reveal the correlation between myoepithelial cell configuration and sizelshape of glandular endpieces (acini), we observed postnatal developmental changes of myoepithelial cells in the lacrimal glands of mice. Glandular and myoepithelial cells were examined in paraffin sections and in isolated acini. In newborns, rudiments of acini showed no clear lumina and glandular cells had few secretory granules. There were no myoepithelial cells with actin. At 3 days after birth, some rudiments showed lumina; however, secretory granules were not salient. Round cells in the periphery of the acini showed immunoreactivity for actin. In l-weekold mice, glandular cells were polarized: luminal cytoplasm contained some secretory granules, and nuclei were located basally. Most myoepithelial cells were flattened and sometimes projected thin processes in various directions. At 2 weeks, the glandular cells increased their size and contained numerous secretory granules, and the myoepithelial cells were almost stellate. In 4-8 week-old mice, acini increased their size, and myoepithelial cells were very thin and processes were prolonged in length and increased in number. During postnatal development, their distribution of myoepithelial cells was more scarce, while the size of acini increased. This reciprocal relation of myoepithelial cell distribution and acinar size may indicate that the changes of myoepithelial cell configurations depend on the change of acinus size.
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    Myofibroblasts and myoepithelial cells in the chicken Harderian gland
    (Murcia : F. Hernández, 1991) Cacho, Emilio del; Gallego, Margarita; Felices, Carlos; Bascuas, J.A.
    An electron microscopic study of the myoepithelial cells in the chicken Harderian gland provides evidence that these cells can be transformed into myofibroblasts. After the application of a Brucella ovis suspension in sterile saline onto the eyeball, every 5 minutes for half an hour, myoepithelial cells gradually develop over a 90-minute period the characteristic features of myofibroblasts: bundles of intracytoplasmic microfilament; abundant rough endoplasmic reticulum; prominent Golgi complex; and surface membrane differentiations, that provide attachment to neighbouring epithelial cells. No typical desmosomes are observed. Besides, the intercellular space between epithelial cells and myofibroblasts increases and the basement membrane adjacent to myofibroblasts disappears. Hypoxia is hypothesized to be involved in the transformation of myoepithelial cells into myofibroblasts.