Browsing by Subject "MMP-1"

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    MMP-1/TIMP-1 expressions in rectal submucosa of females with obstructed defecation syndrome associated with internal rectal prolapse
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Cao, Long-Lei; Yu, Jie; Yang, Zhang-Ling; Qiao, Xu; Ye, Hui; Xi, Chang-Lei; Zhou, Qi-Chang; Hu, Cheng-Cheng; Zhao, Chao-Jun; Gong, Zhi-Lin
    Objective. To explore the MMP-1/TIMP-1 expressions rectal submucosa of females with obstructed defecation syndrome (ODS) associated with internal rectal prolapse (IRP). Methods. Fifty-six female patients with ODS associated with IRP were enrolled as Case group, and 43 female hemorrhoids of stages III-IV without constipation and IRP were enrolled as Control group. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry were performed to test the expressions of MMP1/TIMP-1 in the rectal submucosa. Western blotting was used to examine protein expressions of MMP-1/TIMP-1 and pro-inflammatory cytokines (IL-6 and TNF-α) in the rectal submucosa. EVG staining was conducted to detect collagen and elastic fibers in rectal submucosa. Results. The increased expression of MMP-1 was negatively linked to the decreased TIMP-1 level in the rectal submucosa of patients with ODS associated with IRP. Besides, the expressions of IL-6 and TNF-α were increased in the Case group as compared with the Control group. Additionally, ODS severity and the proinflammatory cytokines was positively linked to MMP1, but negatively related to TIMP-1 in Case group. EVG staining showed that the area ratios of collagen and elastic fibers were lower in Case group than Control group. Through Pearson’s correlation analysis, the area ratios of collagen and elastic fibers were positively associated with MMP-1 expression, but negatively correlated with TIMP-1 expression in rectal submucosa of patients with ODS associated with IRP. Conclusion. Elevated MMP-1 and reduced TIMP-1 were found in ODS associated with IRP, which was related to the ODS severity, inflammation and contents of collagen and elastic fibers.
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    PlGF/Flt-1/MMP-1 axis in gingival carcinoma bone invasion
    (Universidad de Murcia, Departamento de Histología e Histopatología, 2025) Nguyen Phuong Thao; Miyauchi Mutsumi; Subarnbhesaj Ajiravudh; Ogawa Ikuko; Chea Chanbora; Takata Takashi; Biología Celular e Histología
    Background. Gingival squamous cell carcinoma (SCC) frequently invades adjacent bone tissue, leading to significant morbidity and mortality. Understanding the molecular mechanisms driving bone invasion is crucial for developing targeted therapies. We investigated the role of placental growth factor (PlGF) in this process, focusing on its interaction with RANKL and MMP-1. Methods. We examined the role of PlGF in bone invasion of gingival SCC through analysis of patient samples (n=55) and various in-vitro assays, including an in-vitro bone-cell coculture system. We investigated the molecular mechanisms underlying PlGF-mediated bone invasion and its relationship with RANKL and MMP-1 expression. Results. Our findings demonstrate that gingival SCC-secreted PlGF promotes local bone invasion through two possible ways: 1) direct induction of RANKL expression, activating osteoclast formation and bone resorption, and 2) indirect upregulation of RANKL via MMP-1 signaling. PlGF secretion by tumor cells triggered RANKL and MMP-1 production and significantly stimulated migration and osteoclastogenesis (p<0.05). Furthermore, PlGF is highly expressed in gingival SCC and significantly correlated with bone invasion. Finally, we also confirmed the significantly positive correlation between expression levels of MMP-1 with PlGF and Flt-1 expression. Conclusion. This study identifies PlGF as a key regulator of osteoclastogenesis in gingival SCC through both direct and MMP-1-mediated pathways. Therefore, targeting PlGF activity may represent a potential therapeutic strategy for inhibiting bone invasion in gingival SCC