Browsing by Subject "Lymphocyte"

Now showing 1 - 4 of 4
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    Clinical meaning of stromal tumor infiltrating lymphocytes (sTIL) in early luminal B breast cancer
    (MDPI, 2023-05-20) García-Torralba, Esmeralda; Pérez Ramos, Miguel; Ivars Rubio, Alejandra; Navarro-Manzano, Esther; Blaya Boluda, Noel; Morena Barrio, Pilar de la; García Garre, Elisa; Martínez Díaz, Francisco; Chaves-Benito, Asunción; García-Martínez, Elena; Ayala de la Peña, Francisco; Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
    Luminal breast cancer (BC) is associated with less immune activation, and the significance of stromal lymphocytic infiltration (sTIL) is more uncertain than in other BC subtypes. The aim of this study was to investigate the predictive and prognostic value of sTIL in early luminal BC. The study was performed with an observational design in a prospective cohort of 345 patients with predominantly high-risk luminal (hormone receptor positive, HER2 negative) BC and with luminal B features (n = 286), in which the presence of sTIL was analyzed with validated methods. Median sTIL infiltration was 5%(Q1–Q3range(IQR),0–10). We found thatsTIL were associated with characteristics of higher biological and clinical aggressiveness (tumor and lymph node proliferation and stage, among others) and that the percentage of sTIL was predictive of pathologic complete response in patients treated with neoadjuvant chemotherapy (OR: 1.05, 95%CI 1.02–1.09, p < 0.001). The inclusion of sTIL (any level of lymphocytic infiltration: sTIL > 0%) in Cox regression multivariable prognostic models was associated with a shorter relapse-free interval (HR: 4.85, 95%CI 1.33–17.65, p = 0.016) and significantly improved its performance. The prognostic impact of sTIL was independent of other clinical and pathological variables and was mainly driven by its relevance in luminal B BC.
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    Differentiation of human lymphocytes into nuclear vlimata by meiosis. The cytotoxic effect of calcium-activated neutral proteinase inhibitor
    (Murcia : F. Hernández, 1994) Logothetou-Rella, H.
    Phytohaemagglutinin (PHA)-activated lymphocytes differentiated into nuclear vlimata (NVs) in vitro. Lymphocyte attachment was followed by formation and extrusion of cytoplasmic vesicles. nuclear elongation and fragmentation into NVs. NVs and cytoplasmic vesicles were detached and organized into large cell nodules in suspension. Immunocytochemistry showed that T-lymphocytes differentiated mainly to NVs while B-lymphocytes to buds. During differentiation there was a loss or gain of T-antigenicity by either mother or daughter cell. Cytogenetic analysis by chromosomal spreading and in situ techniques showed that NVs do carry chromosomes of hypodiploid or hypohaploid sets. NVs were the result of meiosis stimulated by PHA. A ly~nphocyticp opulation exhibited diploidies attributed to mitosis or symmetrical meiosis and hypodiploidies, hypohaploidies attributed to meiosis. A comparison between NVs and spermatozoa was provided. The inhibitor of calcium-activated neutral proteinase (CANP-I) was cytotoxic to NVs without interfering with NV production. A model was proposed for the action of CANP-I which is a promising agent against autoimmune diseases.
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    Innervation and nerve-immune cell contacts in mouse Peyer's patches
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Al Shalan, Huda A.M.; Hu, Dailun; Greene, Wayne K.; Ma, Bin
    Neural regulation of the function of the gastrointestinal tract (GIT) relies on a delicate balance of the two divisions of its nervous system, namely, the intrinsic and extrinsic divisions. The intrinsic innervation is provided by the enteric nervous system (ENS), whereas the extrinsic innervation includes sympathetic/parasympathetic nerve fibers and extrinsic sensory nerve fibers. In the present study, we used immunofluorescent staining of neurofilament-heavy (NF-H) to reveal the distribution of nerve fibers and their associations with immune cells inside mouse Peyer’s patches (PP), an essential part of gut-associated lymphoid tissue (GALT). Our results demonstrate (1) the presence of an extensive meshwork of NF-H- immunoreactive presumptive nerve fibers in all PP compartments including the lymphoid nodules, interfollicular region, follicle-associated epithelium, and subepithelial dome; (2) close associations/contacts of nerve fibers with blood vessels including high endothelial venules, indicating neural control of blood flow and immune cell dynamics inside the PP; (3) close contacts between nerve fibers/endings and B/T cells and various subsets of dendritic cells (e.g., B220 - , B220 + , CD4 - , CD4 + , CD8 - , and CD8 + ). Our novel findings concerning PP innervation and nerve-immune cell contacts in situ should facilitate our understanding of bi- directional communications between the PNS and GALT. Since the innervation of the gut, including PP, might be important in the pathogenesis and progression of some neurological, infectious, and autoimmune diseases, e.g., prion diseases and inflammatory bowel disease, better knowledge of PNS-immune system interactions in the GALT (including PP) should benefit the development of potential treatments for these diseases via neuroimmune manipulations.
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    Mononuclear cell infiltrate, HLA-Dr expression and proliferation in 37 acoustic schwannomas
    (Murcia : F. Hernández, 1990) Rossi, M.L.; Jones, N.R.; Esiri, M.M.; Havas, L.; Nakamura, N.; Coakham, H.B.
    Frozen sections from 37 schwannomas of the V111 nerve were reacted with a panel of monoclonal antibodies to macrophage, lymphocyte, I-ILA-Dr invariant chain and nuclear proliferation antigens. A moderate number of rnacrophages was demonstrated in 96% of tumours. CD8- and CD4- lymphocytes were detected in slightly smaller numbers in up to 87% and 23% of tumours respectively. B-lyrnphocytes were present in only 2/32 cases and NK-cells were absent from all 16 cases tested. HLA-Dr antigen was expressed by macrophages in most cases and by tumour cells in 13/24 tumours. These findings may represent evidence for a degree of cellular immune response. Occasional cells featuring nuclear proliferation were detected in 15/27 cases.