Browsing by Subject "Lymphatic tissue"

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    Absence of lymphatic vessels in non-functioning bleb capsules of glaucoma drainage devices
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Siggel, Robert; Schroedl, Falk; Dietlein, Thomas; Koch, Konrad R.; Platzl, Christian; Kaser-Eichberger, Alexandra; Cursiefen, Claus; Heindl, Ludwig M.
    Purpose. To evaluate the presence and appearance of blood and lymphatic vessels in nonfunctioning bleb capsules of glaucoma drainage devices (GDD). Materials and methods. Non-functioning (n=14) GDD-bleb capsules of 12 patients were analyzed by immunohistochemistry for blood vessels (CD31, vascular endothelium), lymphatic vessels (lymphatic vessel endothelial hyaluronan receptor-1 [LYVE-1] and podoplanin) and macrophages (CD68). Results. CD31+++ blood vessels and CD68+ macrophages were detected in the outer layer of all specimens. LYVE-1 immunoreactivity was registered in single non-endothelial cells in 8 out of 14 (57%) bleb capsule specimens. Podoplanin-immunoreactivity was detected in all cases, located in cells and profiles of the collagen tissue network of the outer and/or the inner capsule layer. However, a colocalization of LYVE-1 and podoplanin as evidence for lymphatic vessels was not detected. Conclusions. We demonstrate the presence of bloodvessels but absence of lymphatic vessels in nonfunctioning bleb capsules after GDD-implantation. While the absence of lymphatic vessels might indicate a possible reason for drainage device failure, this needs to be confirmed in upcoming studies, including animal experiments.