Browsing by Subject "Hydroxyapatite"

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    Biocompatibility of a HA/β-TCP/C scaffold as a pulp-capping agent for vital pulp treatment: an in vivo study in rat molars
    (MDPI, 2021-04-08) Guerrero Gironés, Julia; Alcaina Lorente, Antonio; Ortiz Ruiz, Clara; Ortiz Ruiz, Eduardo; Pecci Lloret, María P.; Ortiz Ruiz, Antonio José; Rodríguez Lozano, Francisco Javier; Pecci Lloret, Miguel R.; Dermatología, Estomatología, Radiología y Medicina Física
    Bioceramic materials possess desirable biological properties, highlighting their non-reactivity and osteoconductivity. Their use has been extended in vital pulp treatment. The purpose of this study was to evaluate and compare the effects of beta-tricalcium phosphate (β-TCP), hydroxyapatite (HA), and collagen (C) scaffold with mineral trioxide aggregate (MTA) on the vital pulp of rat molars. Thirty-two molars of Sprague–Dawley rats underwent direct pulp capping with β-TCP/HA/C (n = 16) and MTA (n = 16). After 30 days, the following parameters were evaluated in the tested samples: the degree of pulp inflammation and pulp vitality, the presence of reparative dentin, the homogeneity of the odontoblastic layer, and the presence of pulp fibrosis. No statistically significant differences were observed between HA/β-TCP/C and MTA in terms of the degree of inflammation (p = 0.124). Significant differences were found in reparative dentin formation between the treatment groups (p = 0.0005). Dentin bridge formation was observed in the MTA-treated group. The local action of HA/β-TCP/C is similar to that of MTA when used as an agent for pulp vital treatment in terms of absence of inflammation and maintenance of pulp vitality, although there are significant differences between both materials regarding the formation of dentin bridges.
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    Biological response to porcine xenograft implants: an experimental study in rabbits
    (Lippincott Williams & Wilkins, 2012-04) Calvo Guirado, José Luis; Gómez Moreno, Gerardo; Guardia, Javier; Ortiz Ruiz, Antonio José; Piatelli, Adriano; Barone, Antonio; Martínez González, José María; Meseguer Olmo, Luis; López Marí, Laura; Barona Dorado, Cristina; Dermatología, Estomatología, Radiología y Medicina Física
    Purpose: The aim of this study was to evaluate the effect of a new porcine biomaterial and collagen paste in 20 New Zealand rabbits. Materials and Methods: Forty implants using a porcine xenograft made up of 80% corticocancellous collagenated bone particles of ≤300 μm in size were placed in the proximal metaphyseal area of both tibiae. Four periods of time were formed: 1h, 5, 8, and 15 months. After implantation, an anteroposterior and lateral radiological study was carried out. Samples were sectioned at 5 μm and stained using hematoxylin-eosin, Masson's trichromic, and Gordon-Switt reticulin stains. Results: These results confirmed the biocompatibility of this porcine biomaterial-collagen paste; only a few, occasional macrophages and scattered lymphocytes were observed. No fibrosis was observed between the implants and the bone. Moreover, the material was osteoconductive acting as a “scaffold” for bone cells, and there was a progressive increase in bone growth in and around the implants. Conclusion: This new porcine biomaterial-collagen paste seemed to be biocompatible, bioresorbable, and osteoconductive.
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    Bone union formation in the rat mandibular symphysis using hydroxyapatite with or without simvastatin: effects on healthy, diabetic, and osteoporotic rats
    (Springer Heidelberg, 2020-01-11) Camacho Alonso, Fabio; Martínez Ortiz, C.; Plazas Buendía, L.; Mercado Díaz, A.M.; Vilaplana Vivo, C.; Merino, J.J:; Martínez Beneyto, Yolanda; Navarro Cámara, José Antonio; Buendía Marín, Antonio Julián; Dermatología, Estomatología, Radiología y Medicina Física
    Objective The objective is to compare new bone formation in critical defects in healthy, diabetic, and osteoporotic rats filled with hydroxyapatite (HA) alone and HA combined with simvastatin (SV). Materials and methods A total of 48 adult female Sprague-Dawley rats were randomized into three groups (n = 16 per group): Group, 1 healthy; Group 2, diabetics; and Group 3, osteoporotics. Streptozotocin was used to induce type 1 diabetes in Group 2, while bilateral ovariectomy was used to induce osteoporosis in Group 3. The central portion of the rat mandibular symphysis was used as a physiological critical bone defect. In each group, eight defects were filled with HA alone and eightwithHA combined with SV. The animals were sacrificed at 4 and 8 weeks, and the mandibles were processed for micro-computed tomography to analyze radiological union and bone mineral density (BMD); histological analysis of the bone union; and immunohistochemical analysis, which included immunoreactivity of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP-2). Results In all groups (healthy, diabetics, and osteoporotics), the defects filled with HA+ SV presented greater radiological bone union, BMD, histological bone union, and more VEGF and BMP-2 positivity, in comparison with bone defects treated with HA alone. Conclusions Combined application ofHAand SVimproves bone regeneration in mandibular critical bone defects compared with application of HA alone in healthy, diabetic, and osteoporotic rats. Clinical relevance This studymight help to patients with osteoporosis or uncontrolled diabetes type 1, but future studies should be done.