Browsing by Subject "Golgi technique"

Now showing 1 - 3 of 3
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    A Golgi study on the red nucleus in man
    (Murcia : F. Hernández, 1994) Patt, Stephan; Gerhard, Lieselotte; Zill, E.
    The different cell types comprising the human red nucleus (RN) from eight patients without neuronal diseases were investigated using the Golgi- Braitenberg method for long-stored autopsy material. No giant cells were found due to regression of the magnicellular part of the human RN. We found larger (40 - 50 pm) and smaller (30 pm perikaryon size) medium-sized multipolar neurons with long dendrites, mushroom spines and typical dista1 dendritic tufts. The larger medium-sized RN neurons had some brushshaped dendritic end portions which could not be observed in the Golgi studies on various other mammals described in the literature. We additionally found small neurons with a perikaryon size of 15 pm. These cells were thought to be intrinsic neurons similar to those in animal investigations. The neuronal types found in the normal human RN corresponded to those in the parvicellular part of the mammalian RN. Dendritic end brushes, however. are typical only for the human RN.
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    Pathological changes in dendrites of substantia nigra neurons in Parkinson's disease: a Golgi study
    (Murcia : F. Hernández, 1991) Patt, Stephan; Gertz, Hermann-Josef; Gerhard, Lieselotte; Cervós-Navarro, J.
    Neurons of the substantia nigra show severe morphological changes in Parkinson's disease. Pathological alterations of cell bodies have been described, whereas those of neuronal processes have hardly been investigated. Golgi impregnation has been the chosen method for demonstrating neuronal processes and dendritic and somatic spines. We therefore used the Golgi-Braitenberg method to qualitatively and semi-quantitatively study the substantia nigra of eight patients with Parkinson's disease compared with eight control cases. Golgi impregnation of substantia nigra neurons was good in al1 control cases. In full agreement with the analysis of Braak and Braak (1986) three neuronal types within the substantia nigra were found. In cases of Parkinson's disease, severe pathological changes such as decrease of dendritic length, loss of dendritic spines and severa1 types of dendritic varicosities were found only in the melanin-containing pars compacta neurons. Pars reticulata nerve cells were intact. These findings support the predominant role played by the dopaminergic efferent pathway in the degenerative process. The afferent pathway was not affected. This suggests that the substantia nigra lesion is primary in Parkinson's disease. Loss of neurons found in H & E sections corresponded to a lesser amount of impregnated pars compacta neurons in cases with Parkinson's disease when compared to controls. Evidences exist that the duration of the disease may be related to the extent of pathologically altered Golgi-impregnated pars compacta cells. The amount of Lewy bodies in H & E sections corresponded to the quantity of round varicosities in impregnated pars compacta neurons. These round dendritic varicosities were considered to be Lewy body inclusions. They seem to have no influence on the dendritic spine density and morphology in most cases.
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    The growth of non-pyramidal neurons in the primary motor cortex of man: a Golgi study
    (Murcia : F. Hernández, 1997) Prinz, M.; Prinz, B.; Schulz, E.
    In Golgi-Cox-impregnated motor cortex (regio precentralis) of six children (36 weeks of gestation to 13 month after birth) four types of interneurons of laminae 11-VI have been investigated qualitatively and quantitatively and finally, compared with interneurons of a child (3 month of age) with Down's Syndrome. Al1 cell measurements have been made by using a computerized digital morphometric system. In order to study the developmental stage of interneurons in our cases we statistically compared analogue neuron types in the brains. As pararneters for characterizing non-pyramidal neurons served the pattem of dendritic branching, dendritic length for al1 dendritic orders within single dendritic fields, and of the whole neuron. The quantitative morphological study of dendritic arborizations revealed that bitufted and large multipolar intemeurons had significantly more branching material in the postnatal brains than their prenatal counterparts. These differences were due to the increase of both length and number of branches during perinatal period. Such constant increment in dendritic span and branching provides larger receptive areas and that should improve the development of connections in functional intracortical columns. The quantitative parameter area of dendritic fields and number of branching points of the brain with Down's syndrome revealed in comparison with normal brain of the same age differences such as lower dendritic areas and a higher arnount of branching points