Browsing by Subject "Gingivitis"

Now showing 1 - 5 of 5
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    Changes in MMPs and inflammatory cells in experimental gingivitis
    (Murcia : F. Hernández, 2009) Lorencini, Márcio; Silva, Juliete A.F.; de la Hoz, Cristiane L.R.; Carvalho Hernandes, F.; Stach-Machado Dagmar, R.
    In periodontal disease, extensive disorganization of the extracellular matrix promotes the loss of adhesion between the teeth and periodontium. A previous study suggested a reduction in the area occupied by collagen in the gingiva, during the first week of periodontal disease induction, however, the remaining fibers were more compact and thicker. Therefore, it was decided to investigate which of the MMP-2, -9, -14 and RECK, an MMP inhibitor, were involved in these modifications taking place in early gingivitis induced by ligature. The results of gene expression analysis indicated no changes for RECK. MMP-14 showed a reduction at 7 days of inflammation, and there was an immediate increase in MMP-2 gene expression and enzymatic activity, apparently by the stimulation of resident cells such as fibroblasts. A peak of MMP-9 expression 5 days after ligature followed after the peak of enzymatic activity found two days earlier. This pattern was consistent with the kinetics of macrophage and neutrophil recruitment. Immunohistochemistry suggested that MMP-9 was produced by both resident and inflammatory cells. Based on this evidence, it is suggested that extracellular matrix remodeling is related to MMP-2 and -9 production and activation. This allowed us to conclude that the host inflammatory response represents a significant factor for the advance of periodontal diseases.
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    Evaluation of the presence of gingivitis as confounding factor in assessing inflammatory status in serum and saliva of dogs with diabetes mellitus
    (BioMed Central, 2024-03-23) Franco-Martínez, Lorena; Muñoz Prieto, Alberto; Busato, Francesca; Karveliene, Birute; Stadaliene, Inga; Cerón, José J.; Carrillo, Juana D.; García Martínez, Juan Diego; Dabrowski, Roman; Pardo Marín, Luis; Martínez Subiela, Silvia; Tvarijonaviciute, Asta; Medicina y Cirugía Animal
    The aim of this study was to evaluate the changes in the serum and salivary inflammatory markers induced by Diabetes mellitus (DM) in dogs and to assess the possible confounding effect of gingivitis. A panel of 13 cytokines was measured in the serum and saliva of dogs diagnosed with DM and compared with healthy dogs without gingivitis (control group 1; CG1) and dogs with gingivitis but otherwise healthy (control group 2; CG2). The results of the present study showed statistically significantly higher levels of IL-8, KC-like and MCP1 in the serum of dogs with DM compared to CG1 dogs. In the case of saliva, the DM group presented statistically higher GM-CSF, IL6, IL15, and MCP1 levels compared to CG1, and lower KC-like chemokine compared to CG2. Finally, gingivitis produced changes in saliva, with salivary levels of GM-CSF, IL-6, IL-7, IL-15, IP-10, KC-like, IL-10, IL-18, MCP1, TNFα being statistically significantly higher in the saliva of CG2 dogs compared to CG1. The results of the present study indicate that dogs with DM have altered cytokine levels in serum and saliva compared to healthy dogs. In addition, this study highlights the importance of taking oral health into account when determining cytokines in dogs, as gingivitis can significantly alter their concentrations.
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    Measurement of salivary adiponectin concentrations in dogs
    (Wiley , 2014-07-10) Tvarijonaviciute, Asta; Carrillo Sánchez, J. D.; García Martínez, Juan Diego; Tecles Vicente, Fernando; Martínez Subiela, Silvia; German, Alexander J.; Cerón Madrigal, José Joaquín; Medicina y Cirugía Animal; Facultades de la UMU::Facultad de Veterinaria
    Antecedentes: La medición de adiponectina salival podría mejorar la comprensión de la fisiología de esta adipocina y de su papel en diversas condiciones clínicas. Objetivos: El propósito del estudio fue evaluar la utilidad de un kit ELISA humano para adiponectina en la medición de adiponectina salival en perros, comparar las concentraciones de adiponectina en suero y saliva en una población de perros sanos, y evaluar los posibles efectos de la limpieza dental sobre las concentraciones de adiponectina sérica y salival en perros. Métodos: Para la validación analítica, se determinaron la precisión, exactitud y el límite inferior de cuantificación del ensayo utilizando muestras de saliva. Además, se cuantificaron las concentraciones de adiponectina en muestras de suero y saliva de 24 perros sanos, y de 7 perros con gingivitis leve antes y después de un procedimiento de limpieza dental. Resultados: Los ensayos de validación para adiponectina salival presentaron coeficientes de variación inferiores al 15%, y la recuperación osciló entre el 85% y el 120%. En la prueba de linealidad se observó interferencia al medir adiponectina en saliva, pero esto se resolvió diluyendo las muestras 1:4. En perros sanos, las concentraciones de adiponectina salival y sérica se correlacionaron positivamente (r = 0,650; P = 0,009). Tras el procedimiento de limpieza dental, la concentración de adiponectina salival aumentó el día 0 (P = 0,004), pero a los 14 días las concentraciones fueron inferiores a las registradas antes del procedimiento (P = 0,041). Conclusiones: El kit ELISA humano para adiponectina puede utilizarse para medir con precisión y exactitud la adiponectina salival en perros. La adiponectina salival aumentó 24 horas después de la limpieza dental, posiblemente debido a un proceso inflamatorio agudo o a la filtración de adiponectina desde la sangre tras el traumatismo gingival.
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    Preterm birth and/or low birth weight are associated with periodontal disease and the increased placental immunohistochemical expression of inflammatory markers
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2016) Pozo, Elena; Mesa, Francisco; Ikram, Mohamed H.; Puertas, Alberto; Torrecillas-Martínez, Laura; Ortega-Oller, Inmaculada; Magán-Fernández, Antonio; Rodríguez-Martínez, María Dolores; Padial-Molina, Miguel; Sánchez-Fernández, Elena; Galindo-Moreno, Pablo; O’Valle, Francisco
    The objective of this study was to determine whether gynecological and periodontal clinical parameters and the immunohistochemical expression in placental chorionic villi of the markers cyclooxygenase2 (COX-2), interleukin (IL)-1β, vascular endothelial growth factor receptor 1 (VEGFR1), podoplanin, and Heat Shock Protein (HSP70) are associated with preterm birth (PB) and/or low birth weight (LBW) neonates. Material and methods: An observational casecontrol study was performed in 130 puerperal women: mothers of PB/LBW neonates (cases, n=65) and mothers of full-term normal-weight neonates (controls, n=65). Data were gathered from all participants on sociodemographic, gynecological, and periodontal variables and on placental immunohistochemical COX-2, IL-1β, VEGFR1, podoplanin, and HSP70 expression. Results: Among the 42 women with mild/moderate periodontitis or gingivitis, the studied periodontal variables were significantly worse and the placental COX-2 (p=0.043), HSP70 (p=0.001), IL-1β (p=0.001), VEGFR1 (p=0.032), and podoplanin (p=0.058) expressions were significantly higher in the cases than in the controls. In comparison to the mothers without periodontitis, only COX-2 (p=0.026) and VEGFR1 (p=0.005) expressions were significantly increased in those with the disease. Increased COX-2 values were detected in the women with a history of genitourinary infection (p=0.036), premature rupture of membrane (p=0.012), or drug treatment (p=0.050). Conclusions: The etiology of preterm birth and/or low birth weight is multifactorial and involves consumption habits, social-health factors, and infectious episodes. These adverse pregnancy outcomes were associated with periodontitis and the increased placental expression of IL-1β, COX-2, VEGFR1, and HSP70.
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    T cell regulation of the immune response to infection in periodontal diseases
    (Murcia : F. Hernández, 2003) Yamazaki, K.; Yoshie, H.; Seymour, G.J.
    Although T cells have been implicated in the pathogenesis and are considered to be central both in progression and control of the chronic inflammatory periodontal diseases, the precise contribution of T cells to the regulation of tissue destruction has not been fully elucidated. Current dogma suggests that immunity to infection is controlled by distinct T helper 1 (Th1) and T helper 2 (Th2) subsets of T cells classified on the basis of their cytokine profile. Further, a subset of T cells with immunosuppressive function and cytokine profile distinct from Th1 or Th2 has been described and designated as regulatory T cells. Although these regulatory T cells have been considered to maintain selftolerance resulting in the suppression of auto-immune responses, recent data suggest that these cells may also play a role in preventing infection-induced immunopathology. In this review, the role of functional and regulatory T cells in chronic inflammatory periodontal diseases will be summarized. This should not only provide an insight into the relationship between the immune response to periodontopathic bacteria and disease but should also highlight areas of development for potentially new therapeutic modalities.