Browsing by Subject "Genotype MTBDRplus"

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    Usefulness of Genotype MTBDRplus assay in acid-fast bacilli positive smear specimens in Almeria, Spain
    (Elsevier Doyma, 2014-05-05) Martínez-Lirola, Miguel José; Muñoz-Dávila, María José; García-de Viedma, Darío; Cabezas Fernández, Teresa; Luzón García, Pilar; Indal-Tb Group; Genética y Microbiología
    tIntroduction: The urgent need for operational research evaluating test performance in a real-world settinghas been highlighted. The purpose of this study was therefore to evaluate the performance of MTBDRplusassay.Materials: According to the reference method, of the 155 clinical specimens with valid results, 147 weresusceptible to rifampicin (RIF) and isoniazid (INH), with 4 being multi-drug resistant (MDR) and 4 withisolated resistance to isoniazid (INH).Results: The results of the MTBDRplus assay were 100% concordant for the MDR and mono-resistantto INH specimens. However, the MTBDRplus assay showed a resistance pattern to RIF in one specimenwhich was classified as susceptible by the reference method. The majority of the specimens (118/75.6%)were also tested using the MTBDRplus method after culture on Lowenstein-Jensen media, showing 100%agreement with the results of the test directly from the specimens. An MTBDRplus test result was availablewithin an average of 8 days.Conclusions: Overall, MTBDR results showed excellent results when compared with the reference methodand achieved a significant time-reduction.