Browsing by Subject "Fluoride varnishes"

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    Influence of fluoride varnish application on enamel adhesion of a universal adhesive
    (Quintessence Publishing, 2021-01) Ortiz Ruiz, Antonio José; Martínez Marco, Francisco José; Pérez Silva, Amparo; Serna Muñoz, Clara; Cabello, Inmaculada; Banerjee, Avijit; Dermatología, Estomatología, Radiología y Medicina Física
    Purpose: To study the effect of the varnish type, application time and surface polishing on the shear bond strength (SBS) of a universal adhesive in healthy and demineralized bovine enamel. Materials and Methods: 432 bovine primary central incisors were assigned to 18 groups according to enamel mineralization [healthy and demineralized], topical varnish [Clinpro White Varnish (CWV; 3M Oral Care) and Profluorid (PFV, Voco)], remineralization time [24 h or 21 days] and polishing or not of the enamel surface. Adhesion was tested using Futurabond M (Voco)+ and GrandioSO (Voco). Sheer bond strength (SBS) was measured and the fracture mode studied. The statistical analysis was performed using two-way ANOVA, Tukey's test, and Pearson's chi-squared test. Results: In healthy bovine enamel, CWV reduced SBS at 24 h and 21 days; polishing significantly improved SBS. PFV increased SBS in healthy enamel at 21 days and demineralized enamel at 24 h and 21 days; polishing had no effect on SBS. The application time and polishing of the enamel surface affected the behavior of varnishes with respect to SBS. There was a correlation between the type of fracture and the degree of mineralization as well as the timepoint of varnish application. Conclusions: Remineralization of demineralized enamel with fluoride varnishes permits the recovery of the bond strength obtained in healthy enamel. Of the two varnishes studied, PFV had the highest SBS and more uniform behavior, regardless of the application timepoint, degree of mineralization, and surface treatment of the enamel.
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    Topical fluoride varnishes promote several biological responses on human gingival cells
    (Elsevier, 2021-04-18) López García, Sergio; Pecci Lloret, María Pilar; Pecci Lloret, Miguel Ramón; Guerrero Gironés, Julia; Rodríguez Lozano, Francisco Javier; García Bernal, David; Dermatología, Estomatología, Radiología y Medicina Física
    Objective: To compare the in vitro cytotoxicity of four commercial topical fluoride varnishes widely used in daily dental practice for the prevention of caries on human fibroblasts: Cervitec F, Fixofluor, Fluor Protector S and Duraphat. Material and methods: Human gingival fibroblasts (hGF) were exposed to different concentrations of fluoride varnishes extracts. Biological assays, including MTT and IC50 value determination, annexin-V/7- AAD staining, cell migration and F-actin staining with phalloidin were carried out. Statistical analyses were performed using one-way ANOVA and Tukey’s post hoc test. Results: At 4% concentration, all of the fluoride varnishes extracts affected fibroblasts metabolic activity, exhibiting ahighdegree of cytotoxicity at allmeasured time points.At 0.1%and 1%, Duraphat and Fixofluor or Fluor Protector S and Cervitec F exerted the lowest or highest cytotoxic effects, respectively. Similar effects were evidenced when induction of apoptosis/necrosis and cell migration assays were analyzed. Immunocytochemical assays revealed a similarnumber offibroblasts, without changes inthemorphology and F-actin content at 0.1% concentration of alltested materials, while at 1% concentration, Fluor Protector S and Cervitec F showed few cells with aberrant morphology or non-adhered cells, respectively. Conclusions: Different commercial topical fluoride varnishes with the same therapeutic indication may exhibit different biological effects and cytotoxicity on fibroblasts.