Browsing by Subject "Ferritin"
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- PublicationOpen AccessA kinematic analysis of the two types of soccer throw-in techniques(Universidad de Murcia: servicio de publicaciones, 2024) Radwan, Omar Abd-Al-Karem; A’mir, Omar; Fattah, Osama Abdel; Jaber, Osama Hashem; Ermely, Ziyad Mohammad; Al-Saoud, Hasan AsriThis study aimed to identify kinematic variables in two soccer throw-in techniques, examine their relationship to throw distance, and explore differences based on technique. A descriptive approach was used, with a purposive sample of 15 first-grade players from the Hussein Youth Club. Each player performed both types of throw-in techniques, recorded at 60 f/s using a Nikon D3400 camera placed laterally. A total of 30 successful attempts were analyzed using Kinovea 0.8.27 x64. Variables studied included foot distance, skill duration, release velocity and angle, and ball release height. Data were analyzed with SPSS. The results revealed weaknesses in the side throw-in technique, such as a low release angle, though better distances were achieved with the second technique. Significant correlations were found between foot distance, release angle, release velocity, vertical velocity, and throw distance (p<0.05). There were also statistically significant differences in knee angle, moment of throw, and throw distance, favoring the second technique (p<0.05). The sample's technique shows several weaknesses impacting performance. Projectile variables strongly influence throw distance, closely linked to various kinematic factors. Moreover, the throw-in method is crucial in determining the overall distance
- PublicationOpen AccessComparison of bioavailability and transporters gene expression of four iron fortificants added to infant cereals(Elsevier, 2022-09-08) Caballero Valcárcel, Ana M.; López Nicolás, Rubén; Frontela Saseta, María del Carmen; González Bermúdez, Carlos A.; Martínez Gracia, Carmen; Santaella-Pascual, Marina; Tecnología de Alimentos, Nutrición y BromatologíaIron deficiency is one of the most important nutritional disorders around the world. To reduce this health problem in infants, most countries have developed different iron fortification programs as a long-term strategy. However, it is necessary to select the best iron source to get the highest bioavailability with the lowest impact on the organoleptic properties of the foods. The aim of this research was to assess the in vitro bioavailability and intestinal cellular transport of four iron sources (electrolytic iron: EI, ferrous fumarate: FF, a micronized and dispersible ferric pyrophosphate: MDFP, and ferrous sulphate heptahydrated: FSH) used to fortify infant cereals. Fortified cereals were in vitro digested and soluble fractions were then incubated in Caco-2 cells to study iron retention, transport and uptake. Moreover, mRNA expression of iron transporters and ferritin formation were also measured in cells incubated with the iron standard salts. Iron solubility, uptake percentage, transport and uptake efficiencies by the Caco-2 cells were significantly higher in cereals fortified with FSH than with EI, obtaining intermediate values for the other two iron fortificants. MDFP induced higher expression of iron transporters Zip-14 and HEPH than other iron sources. FSH promoted the highest iron storage protein ferritin, followed by FF and MDFP, while EI presented the lowest in vitro bioavailability due to its low solubility. This work demonstrated that iron absorption, storage, and gene expression of transporters depend on the source of iron. These are important challenges to be taken into consideration when assessing the potential of iron fortificants.
- PublicationOpen AccessEffects of prenatal yoga exercise on hepcidin and ferritin levels in pregnant women(Universidad de Murcia: servicio de publicaciones, 2024) Bara, Farida Tandi; As’ad, Suryani; Idris, Irfan; Riu, Deviana Soraya; Ahmad, MardianaPrenatal yoga is a modification of hatha yoga tailored for pregnant women. This exercise is recommended throughout pregnancy. This study aimed to determine the effect of prenatal yoga exercise on the serum levels of hepcidin and ferritin in pregnant women. The study employed a quasi-experimental design with a pre-and post-test. A total of 134second-trimester pregnant women participatedand were divided into two groups (intervention group = 62, control group = 72). The intervention group received prenatal yoga exercises for 8 weeks (twice a week), while the control group attended only the standard pregnancy class. Each group was further divided into subgroups: anemic pregnant women, normal pregnant women, and overweight pregnant women. Demographic data were obtained through a basic questionnaire, while pre-pregnancy BMI data, obstetric history, and pregnancy examinations were sourced from the KIA book. Serum hepcidin levels significantly decreased in pregnant women who participated in prenatal yoga exercises (p=0.002), with the highest significance observed in overweight pregnant women (p=0.001). The average serum ferritin levels increased in all groups but were not statistically significant (p=0.759). Regular prenatal yoga exercises can reduce serum hepcidin levels in pregnant women, with a significant reduction noted in overweight pregnant women. Ferritin levels might be influenced by iron supplementation.
- PublicationOpen AccessFerritin heavy chain as main mediator of preventive effect of metformin against mitochondrial damage induced by doxorubicin in cardiomyocytes(2014-02) Asensio Lopez, Maria del Carmen; Sanchez Mas, Jesus; Pascual Figal, Domingo A.; de la Torre, Carlos; Valdes, Mariano; Lax Pérez, Antonio Manuel; MedicinaThe efficacy of doxorubicin (DOX) as an antitumor agent is greatly limited by the induction of cardiomyopathy, which results from mitochondrial dysfunction and iron-catalyzed oxidative stress in the cardiomyocyte. Metformin (MET) has been seen to have a protective effect against the oxidative stress induced by DOX in cardiomyocytes through its modulation of ferritin heavy chain (FHC), the main iron-storage protein. This study aimed to assess the involvement of FHC as a pivotal molecule in the mitochondrial protection offered by MET against DOX cardiotoxicity. The addition of DOX to adult mouse cardiomyocytes (HL-1 cell line) increased the cytosolic and mitochondrial free iron pools in a time-dependent manner. Simultaneously, DOX inhibited complex I activity and ATP generation and induced the loss of mitochondrial membrane potential. The mitochondrial dysfunction induced by DOX was associated with the release of cytochrome c to the cytosol, the activation of caspase 3, and DNA fragmentation. The loss of iron homeostasis, mitochondrial dysfunction, and apoptosis induced by DOX were prevented by treatment with MET 24h before the addition of DOX. The involvement of FHC and NF-κB was determined through siRNA-mediated knockdown. Interestingly, the presilencing of FHC or NF-κB with specific siRNAs blocked the protective effect induced by MET against DOX cardiotoxicity. These findings were confirmed in isolated primary neonatal rat cardiomyocytes. In conclusion, these results deepen our knowledge of the protective action of MET against DOX-induced cardiotoxicity and suggest that therapeutic strategies based on FHC modulation could protect cardiomyocytes from the mitochondrial damage induced by DOX by restoring iron homeostasis
- PublicationRestrictedInvolvement of ferritin heavy chain in the preventive effect of metformin against doxorubicin-induced cardiotoxicity(Elsevier, 2013-04) Asensio López, Maria del Carmen; Sánchez Mas, Jesús; Pascual Figal, Domingo A; Abenza, Sergio; Perez Martinez, Maria T; Valdés, Mariano; Lax Pérez, Antonio Manuel; MedicinaDoxorubicin is a wide-spectrum chemotherapeutic agent, although a cumulative dose may cause cardiac damage and lead to heart failure. Doxorubicin cardiotoxicity is dependent on the intracellular iron pool and manifests itself by increasing oxidative stress. Our group has recently shown the ability of metformin, an oral antidiabetic with cardiovascular benefits, to protect cardiomyocytes from doxorubicin-induced damage. This work aimed to study whether metformin is able to modulate the expression of ferritin, the major intracellular iron storage protein, in cardiomyocytes and whether it is involved in their protection. The addition of metformin to adult mouse cardiomyocytes (HL-1 cell line) induced both gene and protein expression of the ferritin heavy chain (FHC) in a time-dependent manner. The silencing of FHC expression with siRNAs inhibited the ability of metformin to protect cardiomyocytes from doxorubicin-induced damage, in terms of the percentage of cell viability, the levels of reactive oxygen species, and the activity of antioxidant enzymes (catalase, glutathione peroxidase, and superoxide dismutase). In addition, metformin induced the activation of NF-κB in HL-1 cells, whereas preincubation with SN50, an inhibitor of NF-κB, blocked the upregulation of the FHC and the protective effect mediated by metformin. Taken together, these results provide new knowledge on the protective actions of metformin against doxorubicin-induced cardiotoxicity by identifying FHC and NF-κB as the major mediators of this beneficial effect.
- PublicationOpen AccessSalivary Ferritin Changes in Patients with COVID-19(MDPI, 2021-12-21) Franco Martínez, Lorena; Cerón, José J.; Vicente Romero, Maria R.; Bernal, Enrique; Torres Cantero, Alberto; Tecles, Fernando; Sánchez Resalt, Cristina; Martínez, Mónica; Tvarijonaviciute, Asta; Martínez Subiela, Silvia; Medicina y Cirugía Animal; Tchounwou, Paul B.High ferritin serum levels can be found in patients with macrophage activation syndrome, and increased serum ferritin due to cytokine storm have been reported in severe COVID-19 patients. Saliva is being increasingly used in COVID-19 tests as a diagnostic sample for virus detection and quantification. This study aimed to evaluate the possible changes in ferritin in saliva in COVID-19 patients. In addition, the effects of different inactivation SARS-CoV-2 treatments in ferritin measurements in saliva, the correlation between ferritin in saliva and serum, and the possible effects of correction of ferritin values by total protein were assessed. Ferritin was measured in saliva from healthy (n = 30) and COVID-19 (n = 65) patients with severe, (n = 18) or mild (n = 47) disease, depending on the need for nasal flow oxygen or assisted respiration. Ferritin was also measured in paired serum and saliva samples (n = 32) from healthy and COVID-19 patients. The evaluated inactivation protocols did not affect the assay’s results except the addition of 0.5% SDS. Significantly higher ferritin was found in the saliva of COVID-19 patients (median; 25–75th percentile) (27.75; 9.77–52.2 µg/L), compared with healthy controls (4.21; 2.6–8.08 µg/L). Individuals with severe COVID-19 showed higher ferritin values in saliva (48.7; 18.7–53.9) than mild ones (15.5; 5.28–41.3 µg/L). Significant correlation (r = 0.425; p < 0.001) was found between serum and saliva in ferritin. Ferritin levels were higher in COVID-19 patients in serum and saliva, and the highest values were found in those patients presenting severe symptomatology. In conclusion, ferritin in saliva has the potential to be a biomarker to evaluate severity in patients with COVID-19.
- PublicationOpen AccessSerum ferritin in obese dogs: changes and comparison with other analytes(MDPI, 2023-07-11) Franco Martínez, Lorena; Pardo Marín, Luis; Sánchez Mateos, Laura; Muñoz Prieto, Alberto; García Martínez, Juan Diego; Cerón, José J.; Martínez Subiela, Silvia; Rubio, Camila P.; Tvarijonaviciute, Asta; Medicina y Cirugía AnimalCanine obesity is the most common nutritional disorder and is associated with decreased quality of life and longevity as well as comorbidities including cardiorespiratory, endocrine, oncologic, or orthopaedic disorders. Ferritin is a major acute-phase protein in dogs, increasing during inflammation; however, it could also be affected by other conditions, including trauma, iron metabolism dysregulations, neoplasia, or hypoxia. Higher ferritin levels have been reported in obese humans, but ferritin has not been explored in canine obesity. To evaluate the possible changes in serum ferritin in canine obesity, ferritin levels from lean/normal weight (CG, n = 55) and overweight/obese dogs (OG, n = 37) were measured, together with complete hemogram and biochemical analyses. Statistically significant higher ferritin levels (1.2-fold) were found in OG (median, (interquartile range), 204 (166–227.5) µg/L) in comparison to CG animals (172 (137–210) µg/L)), with median levels of ferritin in OG dogs above the reference range for healthy animals in our laboratory (60–190 µg/L). In addition, statistically significant higher mean corpuscular volume (MCV), mean cell haemoglobin concentration (MCHC), total proteins, globulins, haptoglobin, total ferric fixation capacity (TIBC), alkaline phosphatase (ALP), butyrylcholinesterase (BChE), triglycerides, and calcium were observed in OG in comparison to CG. The higher levels in ferritin, together with higher TBIC, haematocrit, and MCV, could indicate tissue hypoxia in obese dogs.