Browsing by Subject "Ferric"

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    Changes in serum biomarkers of oxidative stress after treatment for canine leishmaniosis in sick dogs
    (Elsevier, 2016-12) Peres Rubio, Camila; Martínez-Subiela, Silvia; Tvarijonaviciute, Asta; Pardo-Marin, Luis; Segarra, S.; Cerón, J.J.; Hernández Ruiz, Josefa; Medicina y Cirugía Animal
    Canine leishmaniosis (CanL) is a zoonotic disease being endemic in several parts of the world. In this studywe investigated the behavior of a panel of biomarkers of oxidative stress in 12 sick dogs naturally infectedby CanL before and at days 30 and 180 of a successful therapy with a standard treatment. The assays totaloxidant status (TOS), trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma(FRAP), cupric reducing antioxidant capacity (CUPRAC), serum thiol and paraoxonase 1 (PON1) wereincluded in the panel. In addition, correlations between biomarkers of oxidative stress and inflammation(C-reactive protein (CRP) and ferritin) and urinary protein:creatinine ratio (UPC) were calculated. SerumCUPRAC, thiol and PON1 significantly increased after treatment and were negatively correlated with CRP,ferritin and UPC. This study demonstrates that biomarkers of oxidative stress, not previously studied inleishmaniosis such as CUPRAC and thiol, can change after a successful treatment for CanL showing apotential for use in monitoring the treatment of this disease
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    Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: An update
    (BioMed Central, 2016-08-15) Peres Rubio, Camila; Martínez-Subiela, Silvia; Tvarijonaviciute, Asta; Cerón, J.J.; Hernández Ruiz, Josefa; Medicina y Cirugía Animal
    The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant response against the free radicals produced in a given disease. Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC) are different assays described to determine TAC of a sample. This review explains the basis of each assay and their application in the determination of TAC in dogs, and also provides selected information about reports in humans for comparative purposes. It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.