Browsing by Subject "Experimental model"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Publication
    Open Access
    Comparison of epoprostenol and Viscum album efficiencies in the treatment of avascular necrosis of the femoral head: An experimental animal study
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2024) Kumbaraci, Mert; Kumbaraci, Banu Sarsik; Yakut, Basak Doganavsargil; Can, Cenk; Basdemir, Gulcin; Aydogdu, Semih
    Background. The aim of our study is to compare the efficacy of epoprostenol and Viscum album in the treatment of femoral head avascular necrosis with an experimental study. Our hypothesis is that Viscum album, which has similar properties to epoprostenol on the vascular system, is as effective as epoprostenol in the treatment of avascular necrosis. Methods. Avascular necrosis was created on the femoral heads of 45 New Zealand type rabbits by surgical vascular deprivation method. The rabbits were divided into 3 groups. Group 1 was designed as a control group, in group 2 Ilomedin (epoprostenol analogue) was administrated to subjects and in group 3, Helixor (Viscum album extract) was administrated. At the end of the study, there were nine subjects in each group. Osteocyte necrosis, bone marrow necrosis, new bone formation and cartilage degeneration were evaluated microscopically. The extent of bone necrosis and repair and involvement of epiphysis, the bone marrow cellularity ratio and trabecular bone volume were investigated. Results. Subchondral necrosis was seen in more animals in the control group (p=0.03). Osteoblastic and osteoclastic activity were more prominent in the Ilomedin group (p=0.25 and 0.07, respectively). It was seen that the cartilages of the subjects in the Helixor and Ilomedin groups were less damaged. In the Ilomedin group, more animals were seen in the chronic phase of the repair process than in the other groups (p=0.07). Bone marrow cellularity was higher in treatment groups (22% and 20,6% for Ilomedin and Helixor, respectively, p=0,04). Trabecular volume was found to be increased in damaged femoral heads in the treatment groups, the highest increased observed in the Helixor group (p=0.01). Conclusion. Viscum album seems to be effective in decreasing the extention of necrosis and protecting the articular cartilage, and epoprostenol in increasing repair and regeneration.
  • Thumbnail Image
    Publication
    Open Access
    Morphological studies of cytotoxic lesions in reversible endotoxic shock
    (Murcia : F. Hernández, 1990) García, R.; Díaz-Laviada, l.; Bosch, M.A.; Abarca, S.; Portolés, M.T.; Risco, C.; Ainaga, M.J.; Pagani, R.; Municio, A.M.; Escalona, J.
    Reversible endotoxic shock was induced in adult rats by intravenous injection of E. coli Olll:B4 lipopolysaccharide (LPS) and the progression of metabolic and morphological alterations was evaluated. Serum samples and biopsies from adrenal gland, liver and lung were studied at different times after LPS injection. Histological changes in these tissues were observed after endotoxin administration, coinciding with both the acute-phase and the recovery-phase of shock (24-72h after LPS injection). Signs of tissue regeneration can be correlated with the regression of some serum parameters to their normal values. Al1 these results indicate that in this experimental model of endotoxic shock, a reversible status was established, which will allow further studies of the endotoxic pathophysiological mechanisms in vivo, avoiding the complexity of the non-reversible process.