Browsing by Subject "Equine"

Now showing 1 - 3 of 3
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    Characterization of glycoconjugates in the secretory epithelium of the equine ampulla ductus deferentis
    (Murcia : F. Hernández, 2008) Parillo, F.; Verini Supplizi, A.
    The present work was undertaken to determine the glycoconjugates secreted by the epithelium of the equine ampulla ductus deferentis, using conventional (PAS, AB pH 2.5, AB pH 1.0) and lectin histochemical procedures in conjunction with enzymatic digestion and chemical treatment. The presence of abundant apical cytoplasmic blebs suggests that the equine ampulla secretes its products mainly in an apocrine manner. Glandular cells secrete neutral and acidic sialylated glycoconjugates as revealed by conventional histochemical procedures. Lectin histochemistry helped us to discover the following histological positive sites: the mucosal cells, the glandular epithelial cells, the apical cytoplasmic blebs and the basal cells. The ampullary secretions contained both glycoproteic material (revealed by Con-A-, LCA-, GSA-II-, WGA-, RCA-I- positivity) and sialomucins (evidenced by the reactivity of GSA-II, SBA, PNA and RCA-I after sialidase digestion) having different functional roles. The mucosal cells reacted with Con-A, LCA, and also with sialidase/GSA-II-, SBA-, PNA-, and RCA-I sequences, contributing to the chemical heterogeneity of ampullary secretions. DBA lectin was a specific marker for basal cells. The results obtained were compared with our previous findings regarding the differences in the lectin binding pattern of the plasma-membrane of equine sperm collected from epididymal cauda and the ampulla ductis deferentis. Our results support other studies that indicate that ampullary secretions are involved in altering the plasma-membrane glycoconjugates of spermatozoa, contributing to their maturation.
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    Comparison of chromaffin cells from several animal sources for their use as an in vitro model to study the mechanism of organophosphorous toxicity
    (Elsevier, 2006-04-28) Romero, D.; Quesada, E.; Sogorb, M. A.; Vilanova, E.; Carrera, V.; García Fernández, Antonio Juan; Ciencias Sociosanitarias
    It had been observed that the chromaffin cells of bovine adrenal medulla contain high levels of neuropathy target esterase (NTE), the esterase whose inhibition and aging is associated with induction of the organophosphorous induced delayed neuropathy. In this study, total esterase and NTE activities, and their inhibition kinetics by OPs are characterized in adrenal medulla of several species in order to find the best source for chromaffin cells. Total esterase activity in membrane fraction of bovine, equine, porcine, ovine and caprine were 6100±840, 4200±270, 5000±120, 28800±3000, and 10800±2400 mU/g tissue, respectively (mean±S.D., n = 3–4). NTE represented around 70%, 24%, 58%, 10% and 24% of the total esterases in the same tissues, respectively. It was deduced that NTE represents between 69% and 89% of the “B-activity” (activity resistant to 40 M paraoxon) in the membrane fraction of all species. The mipafox I50 calculated for 30-min inhibition of NTE at 37 ◦C ranged between 7.4 and 12 M. These values are in the range of that for brain NTE in hen (the usual model for testing OP delayed neurotoxicity). Considering that bovine adrenal medulla contains high NTE activity, that it represents a high proportion of total activity, it is easier to dissect than adrenal medulla from equine, caprine or ovine, and is more readily available than species cited previously, and that its inhibitory properties are similar to the classical hen brain model, it is deduced that bovine adrenal medulla is the most appropriate source of chromaffin cells to study OP toxicity, with porcine as the second alternative. The kinetic properties of chromaffin cell cultures from bovine and porcine were in accordance with their properties in homogenate and subcellular fractions, and they displayed an appropriate stability and viability of the primary culture to be used in in vitro toxicological studies for both mechanistic and testing purposes.
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    Histopathological features of endometritis eosinophilica in mares
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Grimm, Anna Lena; Schoon, Heinz Adolf; Schöniger, Sandra
    Equine endometritis eosinophilica (EE) is rarely described and its diagnostic criteria are not defined. The aim of this study was to characterize histological features of EE. A data base (1995-2013) was searched for biopsies with increased eosinophils. This study included all biopsies with this diagnosis and representative biopsies without this record. The definition of equine EE was based on criteria for EE in women and the results of the determination of physiological numbers of eosinophils within the equine endometrium. EE was diagnosed in 55 mares. Biopsies of 10 mares contained eosinophils exceeding the physiological range, but no EE; the diagnosis of eosinophilic infiltrates (EI) was applied. Those of the remaining mares (n=126) displayed eosinophils within the physiological range (EWPR). An irregular glandular differentiation during the breeding season was detected in 25% of mares with EE, 33% of mares with EI and 24% of the mares with EWPR. Most mares with EE (93%), EI (90%) and EWPR (72%) showed endometrosis; it was high grade in 11% with EE and 7% with EWPR. Endometritis was diagnosed within 56% of mares with EE, 40% of mares with EI and 37% of those with EWPR. In mares with EE suppurative endometritis dominated (58%) and in those with EWPR nonsuppurative endometritis (58%). This study indicates EE as a primary fertility reducing disease. Results suggest an association between eosinophilic infiltration and the presence of neutrophils. Further, they provide the basis for future studies into the pathogenesis, prognosis and therapy of EE.