Browsing by Subject "Chromogranin A"

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    Argyrophilia in ovarian serous tumors. A comparative study in 127 epithelial ovarian tumors
    (Murcia : F. Hernández, 1991) Meisui Lin; Jun Hanai; Hakira Wada; Masami Ozaki; kenji Nasu; Shigeru Okamoto; Keishi Matsumoto
    The distribution of argyrophil cells in epithelial ovarian tumors was studied in 127 cases. The results showed that not only mucinous tumors and endometrioid tumors contained argyrophil cells, but also some serous tumors expressed argyrophilia. 31% of serous tumors including 40% of serous adenocarcinomas contained variable numbers of argyrophil cells. Argyrophilia has been demonstrated in mucinous tumors, endometrioid tumors and Brenner tumors before. However, this is the first time the presence of argyrophilia in serous tumors has been noticed. Moreover, the argyrophil cells in 5 serous carcinomas showed reactivity with Neuroendocrine (chromogranin A) antibody but not with serotonin. The expression pattern of argyrophilia in the serous tumors was different from that of the mucinous tumors; in the former, argyrophil granules appeared in apical portions or throughout the cytoplasm of single or clustered cells. In addition, the argyrophilia in some serous tumors and endometrioid tumors decreased after diastase digestion Ultrastructurally, no typical neurosecretory granule was found in the argyrophilic serous tumors. The findings in this study suggest that argyrophilia could be quite frequently found in ovarian epithelial tumors and in itself is not a very specific differential characteristic of carcinoid tumors. The argyrophilia found in a variety of epithelial ovarian tumors might lend additional support to the histogenesis and close relationship between the common epithelial tumors of the ovary.
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    Effect of repeated administration of lipopolysaccharide on inflammatory and stress markers in saliva of growing pigs
    (Elsevier, 2014-04-16) Campos, Paulo H.R.F.; Gutiérrez Montes, Ana María; Le Floc’h, Nathalie; Cerón Madrigal, José Joaquín; Merlot, Elodie; Escribano Tortosa, Damián; Producción Animal
    Although saliva could be considered to be an ideal biological sample for evaluation of biomarkers relat- ing to stress and inflammatory responses in pigs, little is known about how these might be influenced by the presence of endotoxaemia. In the present study, the response to repeated administrations of li- popolysaccharide (LPS) was investigated, using a panel of salivary stress markers such as chromogranin A (CgA) and cortisol, as well as inflammatory/immune markers such as haptoglobin (Hp), C-reactive protein (CRP) and immunoglobulin A (IgA). Sixteen growing pigs were adapted to experimental conditions for 3 weeks, after which, 10 of the pigs were selected to receive three doses of LPS at 48 h intervals. Saliva samples were taken from all pigs prior to any LPS administration (baseline) and at time points corresponding to 3 h after each injection of LPS (T1, T2 and T3). Results showed that repeated administration of LPS induced significant elevation of salivary markers of hypothalamic-pituitary-adrenal (cortisol) and immune (Hp, CRP and IgA) activity compared to base- line levels (P < 0.05). However, rectal temperature, CRP and cortisol data suggested that the amplitude of the inflammatory response decreased with successive LPS administrations. Thus, measurement of sal- ivary biomarkers could be a practical tool for evaluating the inflammatory response to endotoxaemia in pigs. In the case of chronic inflammatory states, salivary Hp and IgA might be more sensitive markers than CRP or cortisol.
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    Saliva chromogranin A in growing pigs: a study of circadian patterns during daytime and stability under different storage conditions
    (Elsevier, 2014-01-17) Escribano Tortosa, Damián; Gutiérrez Montes, Ana María; Fuentes Rubio, María; Cerón Madrigal, José Joaquín; Producción Animal
    Salivary chromogranin A (CgA) is considered to be a biomarker of activation of the sympatho-adrenome- dullary system, and has recently been proposed as a useful indicator of the acute stress response in pigs. The aim of the present study was to determinate whether salivary CgA concentrations in healthy growing pigs exhibits any circadian pattern during the daytime, and to evaluate its stability under different stor- age conditions. A total of 80 pigs (40 in spring and another 40 in autumn) of two different ages and gen- ders were used. To establish the circadian pattern, saliva samples were collected at 07.00, 11.00, 15.00 and 19.00 h on two consecutive days. Pooled samples were used for the stability study and were mea- sured on the day of sampling and periodically for up to 360 days later. Samples were stored at 4 °C, 20 °C or 80 °C and the effect of repeated freezing and thawing was also evaluated. No circadian pattern was detected for salivary CgA in either season and there were no significant effects of gender or age. However, mean salivary CgA concentrations were significantly higher (P < 0.0001) in the pigs sampled in autumn, compared to those sampled in the spring. Short term storage at 4 °C is recom- mended for up to 2 days, whereas frozen samples can be stored for 1 year at 20 °C or 80 °C, without substantial reduction in CgA values. In addition, samples can be frozen and thawed up to seven times without significant loss of the biomarker.