Browsing by Subject "Carrageenan"
Now showing 1 - 4 of 4
Results Per Page
Sort Options
PublicationOpen AccessBiomarker-based assessment of somatostatin-6 immunomodulation in gilthead Seabream: From serum proteins to skin mucus enzymes(2022-10-20) Jose Carlos Campos Sánchez; Cristóbal Espinosa-Ruiz; Francisco A. Guardiola; Esteban Abad, María de los Ángeles; Guardiola Abellán, Francisco Antonio; Marín Parra, Claudia; Biología Celular e HistologíaThis study evaluated the immunomodulatory role of somatostatin-6 (SST6) in gilthead seabream (Sparus aurata) using a validated inflammation model induced by λ-carrageenan and a comprehensive panel of serum and skin mucus biomarkers. SST6 was administered at 1 nM and 2 nM, and its effects were assessed in these matrices. The λ-carrageenan model triggered a local inflammatory response with reduced peroxidase, esterase, and protease activities in skin mucus, consistent with the resolution phase of innate immunity. SST6 at 1 nM enhanced serum complement activity without affecting acute-phase proteins, suggesting reinforcement of basal immune surveillance in the host. When combined with λ-carrageenan, SST6 promoted an increase in serum immunoglobulin levels and a selective shift in proteinogram fractions (α2-globulin), while further decreasing the oxidative and proteolytic activities in skin mucus. Correlation analysis revealed the coordinated regulation of key mucosal enzymes, reinforcing the functional integration of local immune responses. These results indicate that SST6 facilitates an early transition from innate to adaptive immunity while preserving the integrity of the mucosal barrier. Furthermore, this study provides the first evidence of complement activation by SST6 in fish and highlights the value of integrating biomarker-based tools to monitor immunophysiological responses and test bioactive compounds in aquaculture research.- PublicationOpen AccessDevelopment and characterization of nanocapsules comprising dodecyltrimethylammonium chloride and K-carrageenan.(Elsevier, 2011-03-23) Rosas Durazo, A.; Lizardi, J.; Higuera Ciapara, I.; Argüelles Monal, W.; Goycoolea Valencia, Francisco Martín; Biología Celular e Histología; Facultades de la UMU::Facultad de BiologíaThe aim of this work was to develop and characterize a new type of nanocapsules. To this end, a nanoemulsion bearing an oily core (Miglyol 812) was obtained by spontaneous emulsification and stabilized by dodecyl-trimethylammonium chloride (DTAC), a commercial cationic surfactant; this nanoemulsion was coated with proportionally very small amounts of κ-carrageenan (at molar charge ratios of Z ≤ 0.0045) that interact predominantly by an electrostatic mechanism with the positively charged sites at the polar heads of DTAC at the nanoemulsion's surface to harness nanocapsules of average size ∼250–330 nm and zeta potential (ζ) ranging from ∼+80 to +7 mV. The potential application of the new type of developed nanosystems as drug delivery vehicles has yet to be investigated and fully realized.
- PublicationOpen AccessThe effect of inhaled nitric oxide on the carrageenan-induced paw edema(F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología, 2015) Coelho, Carly Faria; Vieira, Rodolfo P.; Lopes-Martins, Patrícia Sardinha Leonardo; Teixeira, Simone Aparecida; Borbely, Alexandre Urban; Gouvea, Irene María; Frigo, Lucio; Lopes-Martins, Rodrigo Álvaro BrandãoInhaled nitric oxide therapy reaches not only pulmonary vessels, but also other vasculatures, presenting anti-inflammatory effects. Therefore, this study investigated the effects of inhaled nitric oxide on a mice model of carrageenan-induced paw edema. Paw edema was induced in male Swiss mice (20-30 g) by subplantar injection of carrageenan (0.05 ml of a 1% suspension in 0.9% saline). The evaluation of timecourse edema (mililiter) was measured by plethysmometry until 12 h following carrageenan administration. Thirty minutes after carrageenan injection, some groups received inhaled nitric oxide (300 ppm at variable doses and times) or Indometacin (INDO 5 mg/Kg, v.o), while others received sildenafil (1 mg/Kg, i.p) or rolipram (3 mg/Kg, i.p.) with or without inhaled nitric oxide. Paws were assessed for edema levels by plethysmometry, mieloperoxidase activity and histological analysis. Inhaled nitric oxide significantly reduced carrageenan-induced paw edema, mieloperoxidase activity and inflammatory infiltrate, although similar results were also observed in sildenafil and rolipram treated groups. In addition, significant effects between inhaled nitric oxide with pharmacological therapy was observed. Inhaled nitric oxide presents anti-inflammatory effects on carrageenaninduce paw edema, as observed through reduced edema, mieloperoxidase activity and neutrophil infiltration, indicating that inhaled nitric oxide therapy goes beyond lung vascular effects.
- PublicationOpen AccessUse of carrageenan in the screening of natural anti-inflammatory molecules in fish: in vitro effects of Chiliadenus glutinosus extractsCampos-Sánchez, Jose Carlos; Esteban Abad, María de los Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e HistologíaCarrageenan, a high molecular weight sulphated polysaccharide, has been used in the screening of anti-inflammatory molecules in mammals. In this sense, Chiliadenus glutinosus (L.) Fourr., an endemic species of plant has been reported in the Spanish popular medicine due to their anti-inflammatory properties in humans. However, their properties have poorly been studied in fish. In this assay, different concentrations (0, 0.001, 0.01, 0.125, 0.25, 0.5 and 1 mg mL-1) of aqueous, ethanolic and methanolic extracts of this plant were elaborated, and the antioxidant, bactericidal (against four marine fish pathogen) and cytotoxic activity, as well as several immunological parameters in head-kidney leucocytes (HKLs) of gilthead seabream (Sparus aurata) were evaluated in a first experiment to determinate the extract with the best properties. Our results showed a dose-dependent antioxidant activity of each plant extract increasing with the concentration, a species- and extract-specific bactericidal activity, as well as a cytotoxic activity against the PLHC1 tumour cell line by the ethanolic and methanolic extracts. However, taking into account the cytotoxic effects of these two extracts on HKLs and the strong immunosuppressive effects on the immunological parameters assayed (peroxidase, respiratory burst activity and phagocytic capacity), a second experiment was developed by using only adjusted doses of the aqueous extract (0, 0.25 and 0.5 mg mL-1), in which immune parameters, cell morphology and inflammatory-related genes were studied in HKLs stimulated with λ-carrageenan. The present results offer a detailed view of the biological effects of C. glutinosus extracts, which could be interesting to the aquaculture sector due to their possible anti-inflammatory effects.